ES2186008T3 - Colorantes de reticulocito excitables por helio-neon y derivables de halolepidinas. - Google Patents

Colorantes de reticulocito excitables por helio-neon y derivables de halolepidinas.

Info

Publication number
ES2186008T3
ES2186008T3 ES97949549T ES97949549T ES2186008T3 ES 2186008 T3 ES2186008 T3 ES 2186008T3 ES 97949549 T ES97949549 T ES 97949549T ES 97949549 T ES97949549 T ES 97949549T ES 2186008 T3 ES2186008 T3 ES 2186008T3
Authority
ES
Spain
Prior art keywords
excitable
dyes
moiety
heterocyclic
neon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97949549T
Other languages
English (en)
Inventor
Nai-Yi Wang
Alex W C Yem
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2186008T3 publication Critical patent/ES2186008T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Non-Silver Salt Photosensitive Materials And Non-Silver Salt Photography (AREA)
  • Holo Graphy (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA SINTETIZAR COLORANTES EXCITABLE POR UN LASER DE HELIO - NEON (EXCITABLE A 633 NM) A PARTIR DE HALOLEPIDINAS, POR EJEMPLO, LA 7 - HALOLEPIDINA, LOS CUALES SON ADECUADOS PARA LA DETECCION Y ENUMERACION DE RETICULOCITOS EN MUESTRAS DE SANGRE HUMANA. EN OTRO ASPECTO, LA PRESENTE INVENCION PROPORCIONA UN PROCEDIMIENTO PARA LA INMUNOTIPIFICACION DE LOS FENOCITOS. EL PROCEDIMIENTO SE BASA EN EL FENOMENO DE QUE, CUANDO UNOS COLORANTES DE DETERMINADA ESTRUCTURA SE INTERCALAN EN EL ADN O EL ARN, AUMENTA LA INTENSIDAD DE LOS COLORANTES. LOS COLORANTES ADECUADOS PARA ESTA INVENCION PUEDEN DESCRIBIRSE COMO COLORANTES QUE TIENEN: (A) UNA PRIMERA MITAD HETEROCICLICA, (B) UNA SEGUNDA MITAD HETEROCICLICA, Y (C) UN GRUPO DE ENLACE QUE CONECTA LAS MITADES HETEROCICLICAS PRIMERA Y SEGUNDA. AMBAS MITADES HETEROCICLICAS PRIMERA Y SEGUNDA DEBEN CONTENER AL MENOS DOS ANILLOS, PREFERENTEMENTE FUSIONADOS ENTRE SI. EL COLORANTE SE CARACTERIZA POR CONJUGACION, CON LO CUAL LA PRIMERA MITAD SE CONJUGA ETILENICAMENTE A LA SEGUNDA MITAD. LA ESTRUCTURA GENERAL DE COLORANTES ADECUADOS PARA UTILIZAR EN LA PRESENTE INVENCION SE ILUSTRA EN LA FORMULA (I), EN LA CUAL R 1 , R 2 , R 3 , R 4 REPRESENTAN, INDEPENDIENTEMENTE, UN MIEMBRO SELECCIONADO DEL GRUPO FORMADO POR HIDROGENO, HALOGENO, CIANO, ALQUILO, ARILO, ALCARILO, ARALQUILO, O R 1 Y R 2 , TOMADOS CONJUNTAMENTE, O R 2 Y R SUP,3 , TOMADOS CONJUNTAMENTE, O R 3 O R 4 , TOMADOS CON JUNTAMENTE, REPRESENTAN UNO O MAS ANILLOS; R 5 REPRESENTA UN GRUPO ALQUILO O UN GRUPO ARILO; R 6 , R 7 , R 8, R 9 REPRESENTAN, INDEPENDIENTEMENTE, UN MIEMBRO SELECC IONADO DEL GRUPO FORMADO POR HIDROGENO, HALOGENO, CIANO, ALQUILO, ARILO, ALCARILO, ARALQUILO, O R 6 Y R 7 , TOMADOS CONJUNTAMENTE, O R 7 Y R 8 , TOMADOS CONJUNTAMENTE, O R 8 Y R 9 , TOMADOS CONJUNTAMENTE, REPRESENTAN UNO O MAS ANILLOS, A CONDICION DE QUE AL MENOS UNO DE R 6 , R SUP,7 , R 8 , R 9 REPRESENTE HALOGENO; R 10 REPRES ENTA UN GRUPO ALQUILO O UN GRUPO ARILO; R 11 Y R 12 REPRESENTAN, INDEPENDIENTEMENTE, UN MIEMBRO SELECCIONADO DEL GRUPO FORMADO POR HIDROGENO, HALOGENO, CIANO, ALQUILO, ARILO, ALCARILO, ARALQUILO, O R 11 O R 12 , TOMADOS CONJUNTAM ENTE, REPRESENTAN UNO O MAS ANILLOS; R 13 REPRESENTA HIDROGENO O UN GRUPO ALQUILO; Y REPRESENTA S, O, C O SE, A CONDICION DE QUE Y NO SEA N; Y N REPRESENTA UN NUMERO DE 0 A 3.
ES97949549T 1996-12-10 1997-11-19 Colorantes de reticulocito excitables por helio-neon y derivables de halolepidinas. Expired - Lifetime ES2186008T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/763,022 US6495692B1 (en) 1996-12-10 1996-12-10 Helium-neon excitable reticulocyte dyes derivable from halolepidines

Publications (1)

Publication Number Publication Date
ES2186008T3 true ES2186008T3 (es) 2003-05-01

Family

ID=25066680

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97949549T Expired - Lifetime ES2186008T3 (es) 1996-12-10 1997-11-19 Colorantes de reticulocito excitables por helio-neon y derivables de halolepidinas.

Country Status (8)

Country Link
US (2) US6495692B1 (es)
EP (1) EP0944672B1 (es)
JP (2) JP4454046B2 (es)
AT (1) ATE223461T1 (es)
CA (1) CA2273959C (es)
DE (1) DE69715242T2 (es)
ES (1) ES2186008T3 (es)
WO (1) WO1998026007A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
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JP3783808B2 (ja) * 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
US6368864B1 (en) * 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration
CA2380272C (en) * 2000-05-23 2011-02-15 Dyomics Gmbh Stable near-infrared (nir) marker dyes based on benzopyrylium-polymethines
US20050032607A1 (en) * 2002-02-08 2005-02-10 Chun Song Jung Apparatus for preventing sudden acceleration of automobile fitted with automatic transmission
JP2006011535A (ja) * 2004-06-22 2006-01-12 Sony Corp コンテンツ転送装置，コンテンツ転送方法およびコンピュータプログラム
US7598390B2 (en) 2005-05-11 2009-10-06 Life Technologies Corporation Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use
GB0615211D0 (en) * 2006-07-31 2006-09-06 Ge Healthcare Uk Ltd Asymmetric flouro-substituted polymethine dyes
CN101343420B (zh) * 2007-07-12 2013-10-16 大连理工大学 一类不对称菁类荧光染料
CN101349644B (zh) 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
CN101475754A (zh) 2008-01-04 2009-07-08 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
CN101602762B (zh) 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
CN101726579B (zh) 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 血液检测试剂和方法
US8367358B2 (en) 2008-12-17 2013-02-05 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Reagent, kit and method for differentiating and counting leukocytes
CN101988082B (zh) 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法

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DE888046C (de) 1943-08-04 1953-08-27 Agfa Ag Fuer Photofabrikation Verfahren zur Sensibilisierung von Halogensilberemulsionen mit Hilfe von unsymmetrischen Carbocyaninen
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JPS521299B1 (es) * 1967-07-25 1977-01-13
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JPS4949504B1 (es) * 1968-12-04 1974-12-27 Fuji Photo Film Co Ltd
JPS61153631A (ja) * 1984-11-30 1986-07-12 Fuji Photo Film Co Ltd 熱現像カラ−感光材料
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
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US6368864B1 (en) * 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration

Also Published As

Publication number Publication date
JP2009167191A (ja) 2009-07-30
CA2273959A1 (en) 1998-06-18
ATE223461T1 (de) 2002-09-15
US7083982B2 (en) 2006-08-01
EP0944672B1 (en) 2002-09-04
US20030145394A1 (en) 2003-08-07
EP0944672A1 (en) 1999-09-29
DE69715242D1 (de) 2002-10-10
US6495692B1 (en) 2002-12-17
JP2001506686A (ja) 2001-05-22
CA2273959C (en) 2007-07-03
WO1998026007A1 (en) 1998-06-18
JP4454046B2 (ja) 2010-04-21
DE69715242T2 (de) 2003-08-07

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