ATE51078T1 - Verfahren zum faerben von proteinen und nukleinsaeuren mittels silber. - Google Patents
Verfahren zum faerben von proteinen und nukleinsaeuren mittels silber.Info
- Publication number
- ATE51078T1 ATE51078T1 AT85111878T AT85111878T ATE51078T1 AT E51078 T1 ATE51078 T1 AT E51078T1 AT 85111878 T AT85111878 T AT 85111878T AT 85111878 T AT85111878 T AT 85111878T AT E51078 T1 ATE51078 T1 AT E51078T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- silver
- proteins
- staining proteins
- matrix
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 229910052709 silver Inorganic materials 0.000 title 1
- 239000004332 silver Substances 0.000 title 1
- 238000010186 staining Methods 0.000 title 1
- 239000011159 matrix material Substances 0.000 abstract 2
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 abstract 1
- -1 aromatic sulfonic acids Chemical class 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 239000000499 gel Substances 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 150000003512 tertiary amines Chemical class 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
- G01N33/683—Total protein determination, e.g. albumin in urine involving metal ions
- G01N33/6836—Silver staining
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/905—Photochemical activation of reactions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
- Plural Heterocyclic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/652,890 US4575452A (en) | 1984-09-21 | 1984-09-21 | Kit for silver staining proteins and nucleic acids |
| EP85111878A EP0175371B1 (de) | 1984-09-21 | 1985-09-19 | Verfahren zum Färben von Proteinen und Nukleinsäuren mittels Silber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE51078T1 true ATE51078T1 (de) | 1990-03-15 |
Family
ID=24618627
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT85111878T ATE51078T1 (de) | 1984-09-21 | 1985-09-19 | Verfahren zum faerben von proteinen und nukleinsaeuren mittels silber. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US4575452A (de) |
| EP (1) | EP0175371B1 (de) |
| JP (1) | JPS6180053A (de) |
| AT (1) | ATE51078T1 (de) |
| AU (1) | AU4764385A (de) |
| CA (1) | CA1242626A (de) |
| DE (1) | DE3576563D1 (de) |
| DK (1) | DK427985A (de) |
| GR (1) | GR852288B (de) |
| IL (1) | IL76438A0 (de) |
| NZ (1) | NZ213559A (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4703016A (en) * | 1986-05-05 | 1987-10-27 | The United States Of America As Represented By The Department Of Health And Human Services | Silver stain for rapid, quantitative detection of polypeptides and nucleic acids |
| US4946794A (en) * | 1986-12-18 | 1990-08-07 | Protein Databases, Inc. | Visualization of proteins on electrophoresis gels using planar dyes |
| US4812412A (en) * | 1987-02-26 | 1989-03-14 | Health Research Inc. | Standard specimen and method of making and using same |
| JPH0280958A (ja) * | 1988-09-16 | 1990-03-22 | Dai Ichi Pure Chem Co Ltd | 銀染色法及び銀染色剤キツト |
| US5064768A (en) * | 1988-09-16 | 1991-11-12 | Daiichi Pure Chemicals Co., Ltd. | Silver staining technique and kit |
| US6074818A (en) * | 1990-08-24 | 2000-06-13 | The University Of Tennessee Research Corporation | Fingerprinting of nucleic acids, products and methods |
| AU1893192A (en) * | 1991-03-28 | 1992-11-02 | University Of Tennessee Research Corporation, The | Dna silver staining |
| US5422277A (en) * | 1992-03-27 | 1995-06-06 | Ortho Diagnostic Systems Inc. | Cell fixative composition and method of staining cells without destroying the cell surface |
| US5595878A (en) * | 1995-06-02 | 1997-01-21 | Boron Biologicals, Inc. | Detection of biopolymers and biooligomers with boron hydride labels |
| JP2001512306A (ja) | 1997-01-08 | 2001-08-21 | ライフ テクノロジーズ，インコーポレイテッド | タンパク質の産生方法 |
| EP1344055A4 (de) * | 2000-11-20 | 2009-03-25 | Invitrogen Corp | Materialien zur verbesserung der anfärbung von biopolymeren in matrizes |
| US20070026479A1 (en) * | 2005-06-27 | 2007-02-01 | Invitrogen Corporation | Liquid protein markers for native gel electrophoresis |
| JP2009544974A (ja) | 2006-07-21 | 2009-12-17 | ライフ テクノロジーズ コーポレーション | 鮮明に分解する標識タンパク質分子量標準 |
| CN112920174B (zh) * | 2021-02-02 | 2022-11-04 | 上海交通大学 | 一种光敏感型化合物及其制备方法和应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB631184A (en) * | 1946-10-01 | 1949-10-28 | Eastman Kodak Co | Improvements in the processing of photographic materials |
| US2548552A (en) * | 1947-10-04 | 1951-04-10 | Eastman Kodak Co | Acidifying compound for photographic baths |
| CH304715A (de) * | 1951-03-17 | 1955-01-31 | Cassella Farbwerke Mainkur Ag | Verfahren zur Herstellung einer fluoreszierenden heterocyclischen Verbindung. |
| US2696439A (en) * | 1952-01-04 | 1954-12-07 | Levinos Steven | Photographic stabilization process |
| US3148194A (en) * | 1961-07-03 | 1964-09-08 | Arapahoe Chemicals Inc | Certain 1, 4-bis[2(4-substituted, 5-phenyloxazolyl)] benzenes |
| US3326685A (en) * | 1964-04-27 | 1967-06-20 | Eastman Kodak Co | Lithographic process using a stop bath |
| US3622332A (en) * | 1968-04-19 | 1971-11-23 | Itek Corp | Photographic process with improved activator composition |
| US4405720A (en) * | 1981-03-04 | 1983-09-20 | The United States Of America As Represented By The Department Of Health And Human Services | Silver stains for protein in gels |
| US4434234A (en) * | 1981-04-02 | 1984-02-28 | The Upjohn Company | Method and kit for silver staining substances supported in matrix |
| US4416998A (en) * | 1981-04-02 | 1983-11-22 | The Upjohn Company | Silver stain procedure and kit for same |
| US4459356A (en) * | 1982-02-11 | 1984-07-10 | Georgetown University | Radioactive staining of gels to identify proteins |
| US4468466A (en) * | 1982-02-16 | 1984-08-28 | The United States Of America As Represented By The Department Of Health And Human Services | Silver stains for protein in gels--a modified procedure |
-
1984
- 1984-09-21 US US06/652,890 patent/US4575452A/en not_active Expired - Lifetime
-
1985
- 1985-09-17 CA CA000490897A patent/CA1242626A/en not_active Expired
- 1985-09-19 GR GR852288A patent/GR852288B/el unknown
- 1985-09-19 EP EP85111878A patent/EP0175371B1/de not_active Expired - Lifetime
- 1985-09-19 DE DE8585111878T patent/DE3576563D1/de not_active Expired - Lifetime
- 1985-09-19 AT AT85111878T patent/ATE51078T1/de active
- 1985-09-20 IL IL76438A patent/IL76438A0/xx unknown
- 1985-09-20 NZ NZ213559A patent/NZ213559A/en unknown
- 1985-09-20 DK DK427985A patent/DK427985A/da not_active Application Discontinuation
- 1985-09-20 AU AU47643/85A patent/AU4764385A/en not_active Abandoned
- 1985-09-20 JP JP60206713A patent/JPS6180053A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DK427985D0 (da) | 1985-09-20 |
| EP0175371A3 (en) | 1986-10-01 |
| IL76438A0 (en) | 1986-01-31 |
| NZ213559A (en) | 1988-02-12 |
| DE3576563D1 (de) | 1990-04-19 |
| AU4764385A (en) | 1986-03-27 |
| EP0175371B1 (de) | 1990-03-14 |
| US4575452A (en) | 1986-03-11 |
| JPS6180053A (ja) | 1986-04-23 |
| DK427985A (da) | 1986-03-22 |
| CA1242626A (en) | 1988-10-04 |
| EP0175371A2 (de) | 1986-03-26 |
| GR852288B (de) | 1986-01-21 |
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