ATE113960T1 - Proteingewinnung. - Google Patents

Proteingewinnung.

Info

Publication number
ATE113960T1
ATE113960T1 AT86306917T AT86306917T ATE113960T1 AT E113960 T1 ATE113960 T1 AT E113960T1 AT 86306917 T AT86306917 T AT 86306917T AT 86306917 T AT86306917 T AT 86306917T AT E113960 T1 ATE113960 T1 AT E113960T1
Authority
AT
Austria
Prior art keywords
dialysis
denaturant
resolubilized
denaturing agent
free buffer
Prior art date
Application number
AT86306917T
Other languages
English (en)
Inventor
James E Seely
Original Assignee
Mallinckrodt Group Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mallinckrodt Group Inc filed Critical Mallinckrodt Group Inc
Application granted granted Critical
Publication of ATE113960T1 publication Critical patent/ATE113960T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT86306917T 1985-09-09 1986-09-08 Proteingewinnung. ATE113960T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/773,671 US4656255A (en) 1985-09-09 1985-09-09 Protein recovery

Publications (1)

Publication Number Publication Date
ATE113960T1 true ATE113960T1 (de) 1994-11-15

Family

ID=25098959

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86306917T ATE113960T1 (de) 1985-09-09 1986-09-08 Proteingewinnung.

Country Status (9)

Country Link
US (1) US4656255A (de)
EP (1) EP0215625B1 (de)
JP (1) JP2646081B2 (de)
AT (1) ATE113960T1 (de)
CA (1) CA1267497A (de)
DE (1) DE3650135T2 (de)
DK (1) DK430486A (de)
IE (1) IE67259B1 (de)
IL (1) IL79972A (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5332805A (en) * 1984-02-03 1994-07-26 Celltech Limited Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5792450A (en) * 1985-02-05 1998-08-11 Chiron Corporation Purified human CSF-1
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
AU597924B2 (en) * 1985-12-11 1990-06-14 Natinco Nv Solubilization of protein aggregates
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
DE3855986T2 (de) * 1987-05-11 1998-02-05 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US4975529A (en) * 1989-08-18 1990-12-04 Monsanto Company Method of folding somatotropins
US5530100A (en) * 1990-05-07 1996-06-25 Rhone-Poulenc Rorer Pharmaceuticals Inc. Methods for purification of recombinantly produced proteins
US5849694A (en) * 1990-07-16 1998-12-15 Synenki; Richard M. Stable and bioactive modified porcine somatotropin and pharmaceutical compositions thereof
AU674568B2 (en) * 1993-02-04 1997-01-02 Anaphore, Inc. Improved method for the refolding of proteins
US6153406A (en) * 1993-07-23 2000-11-28 North American Vaccine, Inc. Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B
US5437986A (en) * 1994-06-22 1995-08-01 Schering Corporation Extraction of heterologous insoluble proteins from bacteria
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
US6632425B1 (en) 1997-03-20 2003-10-14 Human Genome Sciences, Inc. Chemokine compositions
CN114957378B (zh) * 2022-04-15 2024-02-13 深圳重链生物科技有限公司 疏水性包涵体抗原蛋白的复性方法
CN116178481A (zh) * 2022-11-28 2023-05-30 青岛润达生物科技有限公司 一种包涵体蛋白的复性方法及用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc

Also Published As

Publication number Publication date
JPS6277332A (ja) 1987-04-09
US4656255A (en) 1987-04-07
IE67259B1 (en) 1996-03-06
JP2646081B2 (ja) 1997-08-25
CA1267497C (en) 1990-04-03
DE3650135T2 (de) 1995-06-14
DK430486D0 (da) 1986-09-09
CA1267497A (en) 1990-04-03
EP0215625B1 (de) 1994-11-09
EP0215625A3 (en) 1989-04-12
DE3650135D1 (de) 1994-12-15
IE862384L (en) 1987-03-09
DK430486A (da) 1987-03-10
IL79972A (en) 1991-05-12
IL79972A0 (en) 1986-12-31
EP0215625A2 (de) 1987-03-25

Similar Documents

Publication Publication Date Title
ATE113960T1 (de) Proteingewinnung.
DK426386A (da) Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
DK394786D0 (da) Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
DK0526544T3 (da) Terapeutiske anvendelser af actinbindende forbindelser
UA26375A1 (uk) Спосіб очищеhhя іhсуліhу
ATE119543T1 (de) Produktion von proteinen in aktiver form.
GR852288B (de)
PH31198A (en) Protein recognized by antibodies raised against native P28 of schitosoma mansoni.
NO881904D0 (no) Fremgangsmaate til selektiv immunologisk bestemmelse av intakt prokollagen peptid (type iii) og prokollagen (type iii) i kroppsvaesker, og middel til dets gjennomfoering.
DK38889D0 (da) Onkogen-indkodet polypeptid med vaekstfaktoraktivitet
FI885025A0 (fi) Koagulationshaemmande protein pp4-x, dess framstaellning och anvaendning.
DK447784D0 (da) Immuninterferon
DE3784891D1 (de) Verfahren zur isolierung von saeurestabilen, biologisch aktiven proteinen.
IT1178489B (it) Vettore clonante, procedimento per la sua costruzione e procedimento di concentrazione e di purificazione di proteine prodotte, formate dal vettore clonante
IT8485664A0 (it) Procedimento per il trattamento di liquami di conceria con ricupero di torbide proteiche utilizzabili in zootecnia.
ATE93546T1 (de) Ergaenzungstestverfahren von proteine bindenden enzymen.
FI934079L (fi) Menetelmä fuusioproteiinin valmistamiseksi
SE8402717L (sv) Hiss, i synnerhet handikapphiss

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties