IT1178489B - Vettore clonante, procedimento per la sua costruzione e procedimento di concentrazione e di purificazione di proteine prodotte, formate dal vettore clonante - Google Patents
Vettore clonante, procedimento per la sua costruzione e procedimento di concentrazione e di purificazione di proteine prodotte, formate dal vettore clonanteInfo
- Publication number
- IT1178489B IT1178489B IT21612/84A IT2161284A IT1178489B IT 1178489 B IT1178489 B IT 1178489B IT 21612/84 A IT21612/84 A IT 21612/84A IT 2161284 A IT2161284 A IT 2161284A IT 1178489 B IT1178489 B IT 1178489B
- Authority
- IT
- Italy
- Prior art keywords
- clonant
- vector
- procedure
- concentration
- construction
- Prior art date
Links
- 238000010276 construction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50839183A | 1983-06-29 | 1983-06-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IT8421612A0 IT8421612A0 (it) | 1984-06-26 |
| IT1178489B true IT1178489B (it) | 1987-09-09 |
Family
ID=24022578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT21612/84A IT1178489B (it) | 1983-06-29 | 1984-06-26 | Vettore clonante, procedimento per la sua costruzione e procedimento di concentrazione e di purificazione di proteine prodotte, formate dal vettore clonante |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0148845A1 (it) |
| JP (1) | JPS60501688A (it) |
| AU (1) | AU2860384A (it) |
| IT (1) | IT1178489B (it) |
| WO (1) | WO1985000185A1 (it) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1177378B (it) * | 1984-12-11 | 1987-08-26 | Anic Spa | Vettore plasmidico psm112 ad espressione in bacillus subtilis |
-
1984
- 1984-03-30 WO PCT/US1984/000491 patent/WO1985000185A1/en unknown
- 1984-03-30 JP JP84501741A patent/JPS60501688A/ja active Pending
- 1984-03-30 AU AU28603/84A patent/AU2860384A/en not_active Abandoned
- 1984-03-30 EP EP84901674A patent/EP0148845A1/en not_active Withdrawn
- 1984-06-26 IT IT21612/84A patent/IT1178489B/it active
Also Published As
| Publication number | Publication date |
|---|---|
| WO1985000185A1 (en) | 1985-01-17 |
| JPS60501688A (ja) | 1985-10-11 |
| AU2860384A (en) | 1985-01-25 |
| IT8421612A0 (it) | 1984-06-26 |
| EP0148845A1 (en) | 1985-07-24 |
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