AU2860384A - Cloning vector,method of constructing such,and method of concentrating and purifying product proteins produced by the cloning vector - Google Patents

Cloning vector,method of constructing such,and method of concentrating and purifying product proteins produced by the cloning vector

Info

Publication number
AU2860384A
AU2860384A AU28603/84A AU2860384A AU2860384A AU 2860384 A AU2860384 A AU 2860384A AU 28603/84 A AU28603/84 A AU 28603/84A AU 2860384 A AU2860384 A AU 2860384A AU 2860384 A AU2860384 A AU 2860384A
Authority
AU
Australia
Prior art keywords
cloning vector
concentrating
constructing
proteins produced
purifying product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU28603/84A
Inventor
Gordon L Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIOVEC TECHNOLOGY Inc
Original Assignee
BIOVEC TECHNOLOGY Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIOVEC TECHNOLOGY Inc filed Critical BIOVEC TECHNOLOGY Inc
Publication of AU2860384A publication Critical patent/AU2860384A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
AU28603/84A 1983-06-29 1984-03-30 Cloning vector,method of constructing such,and method of concentrating and purifying product proteins produced by the cloning vector Abandoned AU2860384A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50839183A 1983-06-29 1983-06-29
US508391 1983-06-29

Publications (1)

Publication Number Publication Date
AU2860384A true AU2860384A (en) 1985-01-25

Family

ID=24022578

Family Applications (1)

Application Number Title Priority Date Filing Date
AU28603/84A Abandoned AU2860384A (en) 1983-06-29 1984-03-30 Cloning vector,method of constructing such,and method of concentrating and purifying product proteins produced by the cloning vector

Country Status (5)

Country Link
EP (1) EP0148845A1 (en)
JP (1) JPS60501688A (en)
AU (1) AU2860384A (en)
IT (1) IT1178489B (en)
WO (1) WO1985000185A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1177378B (en) * 1984-12-11 1987-08-26 Anic Spa PSM112 PLASMIDIC VECTOR FOR EXPRESSION IN BACILLUS SUBTILIS

Also Published As

Publication number Publication date
IT1178489B (en) 1987-09-09
IT8421612A0 (en) 1984-06-26
JPS60501688A (en) 1985-10-11
EP0148845A1 (en) 1985-07-24
WO1985000185A1 (en) 1985-01-17

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