DE69634454T2 - Zusammensetzungen und Verfahren mit Bezug auf eine auf natürliche Weise sekretierte Matrix - Google Patents
Zusammensetzungen und Verfahren mit Bezug auf eine auf natürliche Weise sekretierte Matrix Download PDFInfo
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- DE69634454T2 DE69634454T2 DE69634454T DE69634454T DE69634454T2 DE 69634454 T2 DE69634454 T2 DE 69634454T2 DE 69634454 T DE69634454 T DE 69634454T DE 69634454 T DE69634454 T DE 69634454T DE 69634454 T2 DE69634454 T2 DE 69634454T2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/0059—Cosmetic or alloplastic implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/10—Hair or skin implants
- A61F2/105—Skin implants, e.g. artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3633—Extracellular matrix [ECM]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Transplantation (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Vascular Medicine (AREA)
- Biophysics (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US470101 | 1995-06-06 | ||
| US08/470,101 US5830708A (en) | 1995-06-06 | 1995-06-06 | Methods for production of a naturally secreted extracellular matrix |
| PCT/US1996/009482 WO1996039101A1 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69634454D1 DE69634454D1 (de) | 2005-04-14 |
| DE69634454T2 true DE69634454T2 (de) | 2006-05-11 |
Family
ID=23866272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69634454T Expired - Lifetime DE69634454T2 (de) | 1995-06-06 | 1996-06-06 | Zusammensetzungen und Verfahren mit Bezug auf eine auf natürliche Weise sekretierte Matrix |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5830708A (https=) |
| EP (1) | EP0967934B1 (https=) |
| JP (1) | JP3709487B2 (https=) |
| KR (1) | KR19990022216A (https=) |
| AT (1) | ATE290350T1 (https=) |
| AU (1) | AU708090B2 (https=) |
| CA (1) | CA2223892A1 (https=) |
| DE (1) | DE69634454T2 (https=) |
| ES (1) | ES2238076T3 (https=) |
| NZ (1) | NZ311313A (https=) |
| WO (1) | WO1996039101A1 (https=) |
Families Citing this family (120)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6974571B2 (en) * | 1995-03-28 | 2005-12-13 | Thomas Jefferson University | Isolated stromal cells and methods of using the same |
| US6284284B1 (en) | 1995-06-06 | 2001-09-04 | Advanced Tissue Sciences, Inc. | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix |
| US7767452B2 (en) | 1997-02-20 | 2010-08-03 | Kleinsek Don A | Tissue treatments with adipocyte cells |
| AU6662698A (en) * | 1997-02-20 | 1998-09-09 | Gregory S. Keller | Augmentation and repair of dermal, subcutaneous, and vocal cord tissue defects |
| US6225118B1 (en) | 1997-10-01 | 2001-05-01 | Biocure Limited | Multicellular in vitro assay of angiogenesis |
| GB9720987D0 (en) * | 1997-10-01 | 1997-12-03 | Biocure Ltd | A multicellular in vitro assay of angiogenesis |
| US6660301B1 (en) * | 1998-03-06 | 2003-12-09 | Biosphere Medical, Inc. | Injectable microspheres for dermal augmentation and tissue bulking |
| AU3097999A (en) * | 1998-03-18 | 1999-10-11 | University Of Pittsburgh | Chitosan-based composite materials containing glycosaminoglycan for cartilage repair |
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| US6428978B1 (en) | 1998-05-08 | 2002-08-06 | Cohesion Technologies, Inc. | Methods for the production of gelatin and full-length triple helical collagen in recombinant cells |
| EP2295540A1 (en) * | 1998-11-19 | 2011-03-16 | Organogenesis, Inc. | Bioengineered tissue constructs and methods for producing and using them |
| US20020042128A1 (en) | 2000-09-01 | 2002-04-11 | Bowlin Gary L. | Electroprocessed fibrin-based matrices and tissues |
| US7615373B2 (en) | 1999-02-25 | 2009-11-10 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed collagen and tissue engineering |
| US6662805B2 (en) | 1999-03-24 | 2003-12-16 | The Johns Hopkins University | Method for composite cell-based implants |
| US20030007954A1 (en) | 1999-04-12 | 2003-01-09 | Gail K. Naughton | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
| US6372494B1 (en) | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| EP1196206A1 (en) * | 1999-07-09 | 2002-04-17 | Advanced Tissue Sciences, Inc. | Human naturally secreted extracellular matrix-coated device |
| WO2001014527A1 (en) * | 1999-08-23 | 2001-03-01 | Organogenesis Inc. | Skin care compositions and treatments |
| US7799325B2 (en) * | 1999-11-05 | 2010-09-21 | Kleinsek Donald A | Removal of hypertrophic scars |
| US20080286242A2 (en) * | 1999-11-05 | 2008-11-20 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including mesenchymal cells |
| JP2003517858A (ja) * | 1999-11-05 | 2003-06-03 | ジェリジーン メディカル コーポレーション | 老齢関連の軟組織の欠陥の増強と修復 |
| US20090074729A2 (en) * | 1999-11-05 | 2009-03-19 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including fibroblasts |
| US20080267923A2 (en) * | 1999-11-05 | 2008-10-30 | Donald Kleinsek | Hair undifferentiated cells |
| US7087431B2 (en) | 2000-03-02 | 2006-08-08 | University Of Rochester | Ex vivo generation of functional leukemia cells in a three-dimensional bioreactor |
| EP1274472A2 (en) | 2000-03-20 | 2003-01-15 | Biosphere Medical, Inc. | Injectable and swellable microspheres for tissue bulking |
| US7338657B2 (en) * | 2001-03-15 | 2008-03-04 | Biosphere Medical, Inc. | Injectable microspheres for tissue construction |
| US6436424B1 (en) * | 2000-03-20 | 2002-08-20 | Biosphere Medical, Inc. | Injectable and swellable microspheres for dermal augmentation |
| AU2001247507A1 (en) | 2000-03-20 | 2001-10-03 | Biosphere Medical, Inc. | Injectable microspheres for tissue construction |
| US8366787B2 (en) * | 2000-08-04 | 2013-02-05 | Depuy Products, Inc. | Hybrid biologic-synthetic bioabsorbable scaffolds |
| US6638312B2 (en) | 2000-08-04 | 2003-10-28 | Depuy Orthopaedics, Inc. | Reinforced small intestinal submucosa (SIS) |
| WO2002014480A2 (en) * | 2000-08-16 | 2002-02-21 | Duke University | Decellularized tissue engineered constructs and tissues |
| US20090130066A1 (en) * | 2000-11-06 | 2009-05-21 | Gerigene Medical Corporation | Augmentation and repair of sphincter defects with cells including muscle cells |
| EP1345743B1 (en) * | 2000-11-17 | 2008-07-23 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed collagen |
| US7311905B2 (en) | 2002-02-13 | 2007-12-25 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells |
| DK1349918T3 (da) | 2000-12-06 | 2014-11-10 | Anthrogenesis Corp | Fremgangsmåde til indsamling af stamceller fra moderkagen |
| US7041868B2 (en) | 2000-12-29 | 2006-05-09 | Kimberly-Clark Worldwide, Inc. | Bioabsorbable wound dressing |
| EP3246396B1 (en) | 2001-02-14 | 2020-01-29 | Celularity, Inc. | Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells |
| EP2316919B1 (en) | 2001-02-14 | 2015-10-07 | Anthrogenesis Corporation | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| JP2005515802A (ja) | 2001-07-16 | 2005-06-02 | デピュイ・プロダクツ・インコーポレイテッド | 混成生物／合成品型多孔質細胞外基質支持骨格 |
| US8025896B2 (en) | 2001-07-16 | 2011-09-27 | Depuy Products, Inc. | Porous extracellular matrix scaffold and method |
| AU2002354911B2 (en) | 2001-07-16 | 2007-08-30 | Depuy Products, Inc. | Meniscus regeneration device and method |
| WO2003007839A2 (en) * | 2001-07-16 | 2003-01-30 | Depuy Products, Inc. | Devices form naturally occurring biologically derived |
| WO2003007805A2 (en) * | 2001-07-16 | 2003-01-30 | Depuy Products, Inc. | Cartilage repair apparatus and method |
| WO2003007786A2 (en) | 2001-07-16 | 2003-01-30 | Depuy Products, Inc. | Porous delivery scaffold and method |
| EP1416886A4 (en) * | 2001-07-16 | 2007-04-18 | Depuy Products Inc | SCAFFOLD AND METHOD FOR CARTILAGE REPAIR AND REGENERATION |
| US7819918B2 (en) * | 2001-07-16 | 2010-10-26 | Depuy Products, Inc. | Implantable tissue repair device |
| US7163563B2 (en) | 2001-07-16 | 2007-01-16 | Depuy Products, Inc. | Unitary surgical device and method |
| EP2186407A1 (en) | 2002-02-13 | 2010-05-19 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta and uses and methods of treatment using said cells |
| AU2003219830A1 (en) * | 2002-02-22 | 2003-09-09 | Ebi, L.P. | Methods and compositions for treating bone or cartilage defects |
| US7498171B2 (en) | 2002-04-12 | 2009-03-03 | Anthrogenesis Corporation | Modulation of stem and progenitor cell differentiation, assays, and uses thereof |
| KR100527623B1 (ko) * | 2002-06-01 | 2005-11-15 | 라이프코드인터내셔날 주식회사 | 세포외기질을 포함하는 인공장기 제조용 생분해성 고분자기질 및 그의 제조방법 |
| AU2003298775B2 (en) | 2002-11-26 | 2008-07-17 | Anthrogenesis Corporation | Cytotherapeutics, cytotherapeutic units and methods for treatments using them |
| IL156945A0 (en) * | 2003-07-15 | 2004-02-08 | Itzhak Tavori | Device and a method for orthopedic delivery of bone reconstruction medium |
| US7326571B2 (en) * | 2003-07-17 | 2008-02-05 | Boston Scientific Scimed, Inc. | Decellularized bone marrow extracellular matrix |
| JP2005211099A (ja) * | 2004-01-27 | 2005-08-11 | Olympus Corp | 人工臓器構成材および人工臓器構成体の製造方法および人工臓器 |
| US7569233B2 (en) | 2004-05-04 | 2009-08-04 | Depuy Products, Inc. | Hybrid biologic-synthetic bioabsorbable scaffolds |
| CA2566765C (en) * | 2004-05-11 | 2011-08-23 | Synthasome Inc. | Hybrid tissue scaffolds containing extracellular matrix |
| US20070190108A1 (en) * | 2004-05-17 | 2007-08-16 | Arindam Datta | High performance reticulated elastomeric matrix preparation, properties, reinforcement, and use in surgical devices, tissue augmentation and/or tissue repair |
| AU2005279934A1 (en) * | 2004-08-30 | 2006-03-09 | Theregen, Inc. | Compositions and methods of promoting hair growth |
| US20080226604A9 (en) * | 2005-06-17 | 2008-09-18 | Theregen Company | Methods for treating ischemic tissue |
| US7513866B2 (en) | 2004-10-29 | 2009-04-07 | Depuy Products, Inc. | Intestine processing device and associated method |
| US7354627B2 (en) | 2004-12-22 | 2008-04-08 | Depuy Products, Inc. | Method for organizing the assembly of collagen fibers and compositions formed therefrom |
| US7595062B2 (en) | 2005-07-28 | 2009-09-29 | Depuy Products, Inc. | Joint resurfacing orthopaedic implant and associated method |
| US20070065415A1 (en) * | 2005-09-16 | 2007-03-22 | Kleinsek Donald A | Compositions and methods for the augmentation and repair of defects in tissue |
| DK1957633T3 (en) | 2005-10-13 | 2014-03-17 | Anthrogenesis Corp | Immunomodulation USING PLACE SPEECH STEM CELLS |
| JP5409009B2 (ja) * | 2005-12-01 | 2014-02-05 | エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ | 組織工学の足場としての三次元再構成細胞外マトリクス |
| CN101389754A (zh) | 2005-12-29 | 2009-03-18 | 人类起源公司 | 胎盘干细胞和第二来源干细胞的联合培养 |
| PT2471904T (pt) | 2005-12-29 | 2019-02-25 | Celularity Inc | População de células estaminais placentárias |
| US20070178137A1 (en) * | 2006-02-01 | 2007-08-02 | Toby Freyman | Local control of inflammation |
| ZA200902233B (en) | 2006-10-23 | 2010-07-28 | Anthrogenesis Corp | Methods and compositions for treatment of bone defects with placental cell populations |
| US7871440B2 (en) | 2006-12-11 | 2011-01-18 | Depuy Products, Inc. | Unitary surgical device and method |
| EP3763376A1 (en) | 2007-02-12 | 2021-01-13 | Celularity, Inc. | Treatment of inflammatory diseases using placental stem cells |
| AU2008216748A1 (en) | 2007-02-12 | 2008-08-21 | Anthrogenesis Corporation | Hepatocytes and chondrocytes from adherent placental stem cells; and CD34+, CD45- placental stem cell-enriched cell populations |
| US9200253B1 (en) | 2007-08-06 | 2015-12-01 | Anthrogenesis Corporation | Method of producing erythrocytes |
| NZ599825A (en) | 2007-09-28 | 2014-10-31 | Anthrogenesis Corp | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells |
| KR20170116221A (ko) | 2007-11-07 | 2017-10-18 | 안트로제네시스 코포레이션 | 조산 합병증의 치료에 있어서의 제대혈의 용도 |
| KR101046188B1 (ko) | 2007-11-30 | 2011-07-04 | 주식회사 알앤엘바이오 | 탈락막 또는 지방 유래 줄기세포를 함유하는 요실금의 세포치료제 |
| WO2009073178A1 (en) * | 2007-12-03 | 2009-06-11 | Sanbio, Inc. | Extracellular matrix from pluripotent cells |
| SG10201500237TA (en) | 2008-01-30 | 2015-04-29 | Histogen Inc | Extracellular matrix compositions |
| ES2729962T3 (es) * | 2008-07-02 | 2019-11-07 | Allergan Inc | Composiciones para el relleno y la regeneración de tejido blando |
| KR20240052847A (ko) | 2008-08-20 | 2024-04-23 | 셀룰래리티 인코포레이티드 | 개선된 세포 조성물 및 그의 제조 방법 |
| WO2010056378A2 (en) * | 2008-11-14 | 2010-05-20 | Histogen, Inc. | Extracellular matrix compositions for the treatment of cancer |
| AU2009316541B2 (en) | 2008-11-19 | 2015-08-06 | Celularity Inc. | Amnion derived adherent cells |
| DK2451964T3 (en) | 2009-07-10 | 2018-04-30 | Histogen Inc | Conditioned medium and extracellular matrix compositions from cells grown under hypoxic conditions |
| EP2521535B1 (en) | 2010-01-05 | 2018-04-04 | Cell Constructs I, Llc | Biomaterials made from human hair |
| DK3284818T3 (da) | 2010-01-26 | 2022-06-20 | Celularity Inc | Behandling af knoglerelateret kræft ved hjælp af placenta stamceller |
| LT2556145T (lt) | 2010-04-07 | 2016-11-10 | Anthrogenesis Corporation | Angiogenezė naudojant placentos kamienines ląsteles |
| ES2666746T3 (es) | 2010-07-13 | 2018-05-07 | Anthrogenesis Corporation | Métodos para generar linfocitos citolíticos naturales |
| EP2799077A1 (en) | 2010-11-12 | 2014-11-05 | Allergan, Inc. | Metabolized conditioned growth medium and methods of use |
| AR084754A1 (es) | 2010-12-30 | 2013-06-05 | Anthrogenesis Corp | Composiciones que comprenden celulas madre de placenta y plasma rico en plaquetas, y sus metodos de uso |
| US20120171180A1 (en) | 2010-12-30 | 2012-07-05 | Sascha Abramson | Compositions comprising amnion derived adherent cells and platelet-rich plasma |
| US8969315B2 (en) | 2010-12-31 | 2015-03-03 | Anthrogenesis Corporation | Enhancement of placental stem cell potency using modulatory RNA molecules |
| US9556414B2 (en) | 2011-01-06 | 2017-01-31 | Humacyte | Tissue-engineered constructs |
| JP6104896B2 (ja) | 2011-06-01 | 2017-03-29 | アントフロゲネシス コーポレーション | 胎盤幹細胞を使用する疼痛の治療 |
| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP4249605B1 (en) | 2011-12-22 | 2024-08-28 | President And Fellows Of Harvard College | Methods for analyte detection |
| US9430614B2 (en) * | 2012-05-09 | 2016-08-30 | Koninklijke Philips N.V. | Interventional information brokering medical tracking interface |
| US9914967B2 (en) | 2012-06-05 | 2018-03-13 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using DNA origami probes |
| CN115137753A (zh) | 2013-02-05 | 2022-10-04 | 细胞结构公司 | 来自胎盘的自然杀伤细胞 |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| SG10201913068PA (en) | 2013-06-04 | 2020-02-27 | Harvard College | Rna-guided transcriptional regulation |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| AU2016349288A1 (en) | 2015-11-03 | 2018-05-31 | President And Fellows Of Harvard College | Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix |
| EP4613756A3 (en) | 2016-04-25 | 2025-11-12 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CN105920679B (zh) * | 2016-04-26 | 2018-10-19 | 青岛大学 | 一种具有三维梯度孔结构的皮肤支架材料的制备方法 |
| CN118853848A (zh) | 2016-08-31 | 2024-10-29 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
| CN118389650A (zh) | 2016-08-31 | 2024-07-26 | 哈佛学院董事及会员团体 | 生成用于通过荧光原位测序检测的核酸序列文库的方法 |
| US11185568B2 (en) | 2017-04-14 | 2021-11-30 | President And Fellows Of Harvard College | Methods for generation of cell-derived microfilament network |
| US11718829B2 (en) | 2017-07-28 | 2023-08-08 | Breakthrough Tech Llc | Methods and compositions for manufacturing extracellular matrix |
| US20210038652A1 (en) | 2018-01-30 | 2021-02-11 | Histogen, Inc. | Extracellular vesicles derived from cells cultured under hypoxic conditions and uses thereof |
| CN112770776B (zh) | 2018-07-30 | 2025-08-19 | 瑞德库尔有限责任公司 | 用于样品处理或分析的方法和系统 |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN111084788A (zh) * | 2018-10-22 | 2020-05-01 | 上海蓝盎电子科技发展有限公司 | 人工细胞间质 |
| EP4097251A1 (en) | 2020-01-29 | 2022-12-07 | 10X Genomics, Inc. | Compositions and methods for analyte detection |
| US12077780B2 (en) | 2020-02-14 | 2024-09-03 | Allergan Sales, Llc | Conditioned medium from cells cultured under hypoxic conditions and uses thereof |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| WO2023030435A1 (en) | 2021-09-01 | 2023-03-09 | Shanghai Qisheng Biological Preparation Co., Ltd. | Cartilage regeneration using injectable, in situ polymerizable collagen compositions containing chondrocytes or stem cells |
| US12304974B2 (en) | 2021-12-28 | 2025-05-20 | Shanghai Qisheng Biological Preparation Co., Ltd. | Hyaluronic acid-collagen copolymer compositions and medical applications thereof |
| CN115006597B (zh) * | 2022-06-02 | 2024-01-19 | 上海威高医疗技术发展有限公司 | 一种口腔修复膜及其制备方法 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3949073A (en) * | 1974-11-18 | 1976-04-06 | The Board Of Trustees Of Leland Stanford Junior University | Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution |
| US4424208A (en) * | 1982-01-11 | 1984-01-03 | Collagen Corporation | Collagen implant material and method for augmenting soft tissue |
| US4582640A (en) * | 1982-03-08 | 1986-04-15 | Collagen Corporation | Injectable cross-linked collagen implant material |
| US4837285A (en) * | 1984-03-27 | 1989-06-06 | Medimatrix | Collagen matrix beads for soft tissue repair |
| US5288617A (en) * | 1984-10-31 | 1994-02-22 | Commonwealth Scientific And Industrial Research Organization | Method of producing an antigenic preparation |
| US5266480A (en) * | 1986-04-18 | 1993-11-30 | Advanced Tissue Sciences, Inc. | Three-dimensional skin culture system |
| US4963489A (en) * | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5032508A (en) * | 1988-09-08 | 1991-07-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US4721096A (en) * | 1986-04-18 | 1988-01-26 | Marrow-Tech Incorporated | Process for replicating bone marrow in vitro and using the same |
| US5460939A (en) * | 1986-04-18 | 1995-10-24 | Advanced Tissue Sciences, Inc. | Temporary living skin replacement |
| US5160490A (en) * | 1986-04-18 | 1992-11-03 | Marrow-Tech Incorporated | Three-dimensional cell and tissue culture apparatus |
| US4803075A (en) * | 1986-06-25 | 1989-02-07 | Collagen Corporation | Injectable implant composition having improved intrudability |
| JPH041118Y2 (https=) * | 1986-07-31 | 1992-01-14 | ||
| GB8708009D0 (en) * | 1987-04-03 | 1987-05-07 | Clayton Found Res | Injectable soft tissue augmentation materials |
| US5342765A (en) * | 1991-08-01 | 1994-08-30 | Occidental Chemical Corporation | Method of producing extracellular products from aerobic microorganisms |
| US5204382A (en) * | 1992-02-28 | 1993-04-20 | Collagen Corporation | Injectable ceramic compositions and methods for their preparation and use |
| US5478739A (en) * | 1992-10-23 | 1995-12-26 | Advanced Tissue Sciences, Inc. | Three-dimensional stromal cell and tissue culture system |
| US5541106A (en) * | 1993-04-05 | 1996-07-30 | Desmos, Inc. | Cell matrix stimulated attachment and hemidesmosome assembly |
| US5397352A (en) * | 1993-08-27 | 1995-03-14 | Burres; Steven | Method of recollagenation |
| US5516532A (en) * | 1994-08-05 | 1996-05-14 | Children's Medical Center Corporation | Injectable non-immunogenic cartilage and bone preparation |
-
1995
- 1995-06-06 US US08/470,101 patent/US5830708A/en not_active Expired - Lifetime
-
1996
- 1996-06-06 NZ NZ311313A patent/NZ311313A/xx unknown
- 1996-06-06 JP JP50179097A patent/JP3709487B2/ja not_active Expired - Lifetime
- 1996-06-06 ES ES96921369T patent/ES2238076T3/es not_active Expired - Lifetime
- 1996-06-06 AU AU62609/96A patent/AU708090B2/en not_active Expired
- 1996-06-06 KR KR1019970708695A patent/KR19990022216A/ko not_active Ceased
- 1996-06-06 CA CA002223892A patent/CA2223892A1/en not_active Abandoned
- 1996-06-06 WO PCT/US1996/009482 patent/WO1996039101A1/en not_active Ceased
- 1996-06-06 AT AT96921369T patent/ATE290350T1/de not_active IP Right Cessation
- 1996-06-06 DE DE69634454T patent/DE69634454T2/de not_active Expired - Lifetime
- 1996-06-06 EP EP96921369A patent/EP0967934B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2238076T3 (es) | 2005-08-16 |
| EP0967934B1 (en) | 2005-03-09 |
| DE69634454D1 (de) | 2005-04-14 |
| CA2223892A1 (en) | 1996-12-12 |
| NZ311313A (en) | 2000-05-26 |
| ATE290350T1 (de) | 2005-03-15 |
| JP3709487B2 (ja) | 2005-10-26 |
| EP0967934A4 (en) | 2001-11-28 |
| WO1996039101A1 (en) | 1996-12-12 |
| AU708090B2 (en) | 1999-07-29 |
| JPH11507558A (ja) | 1999-07-06 |
| EP0967934A1 (en) | 2000-01-05 |
| AU6260996A (en) | 1996-12-24 |
| US5830708A (en) | 1998-11-03 |
| KR19990022216A (ko) | 1999-03-25 |
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