DE69620766D1 - Verfahren zur herstellung von polypeptidabkömmlingen - Google Patents

Verfahren zur herstellung von polypeptidabkömmlingen

Info

Publication number
DE69620766D1
DE69620766D1 DE69620766T DE69620766T DE69620766D1 DE 69620766 D1 DE69620766 D1 DE 69620766D1 DE 69620766 T DE69620766 T DE 69620766T DE 69620766 T DE69620766 T DE 69620766T DE 69620766 D1 DE69620766 D1 DE 69620766D1
Authority
DE
Germany
Prior art keywords
polypeptide
dna
homologous
encoding
circular plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69620766T
Other languages
English (en)
Other versions
DE69620766T2 (de
Inventor
Sigurd Okkels
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE69620766D1 publication Critical patent/DE69620766D1/de
Publication of DE69620766T2 publication Critical patent/DE69620766T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69620766T 1995-08-11 1996-08-12 Verfahren zur herstellung von polypeptidabkömmlingen Expired - Lifetime DE69620766T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DK90795 1995-08-11
DK90795 1995-08-11
DK104795 1995-09-20
DK104795 1995-09-20
PCT/DK1996/000343 WO1997007205A1 (en) 1995-08-11 1996-08-12 Method for preparing polypeptide variants

Publications (2)

Publication Number Publication Date
DE69620766D1 true DE69620766D1 (de) 2002-05-23
DE69620766T2 DE69620766T2 (de) 2004-11-18

Family

ID=26064882

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69620766T Expired - Lifetime DE69620766T2 (de) 1995-08-11 1996-08-12 Verfahren zur herstellung von polypeptidabkömmlingen

Country Status (9)

Country Link
US (2) US20030148441A1 (de)
EP (2) EP0843725B1 (de)
JP (1) JP4156666B2 (de)
CN (1) CN1128875C (de)
AT (1) ATE216427T1 (de)
AU (2) AU6655496A (de)
DE (1) DE69620766T2 (de)
DK (1) DK0843725T3 (de)
WO (2) WO1997007205A1 (de)

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US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
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DK0973940T3 (da) 1997-03-18 2008-10-20 Novozymes As En in vitro-fremgangsmåde til konstruktion af et DNA-bibliotek
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ES2358092T3 (es) 2003-10-10 2011-05-05 Novozymes A/S Variantes de proteasa.
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CN1922314B (zh) 2004-02-25 2010-05-05 诺维信公司 真菌细胞壁降解酶
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Also Published As

Publication number Publication date
EP0843725A1 (de) 1998-05-27
WO1997007205A1 (en) 1997-02-27
CN1128875C (zh) 2003-11-26
AU6655396A (en) 1997-03-12
EP1213350A2 (de) 2002-06-12
JPH11510700A (ja) 1999-09-21
US20050048649A1 (en) 2005-03-03
DK0843725T3 (da) 2002-08-12
ATE216427T1 (de) 2002-05-15
AU6655496A (en) 1997-03-12
EP1213350A3 (de) 2002-12-04
CN1192782A (zh) 1998-09-09
DE69620766T2 (de) 2004-11-18
EP0843725B1 (de) 2002-04-17
US20050124066A9 (en) 2005-06-09
JP4156666B2 (ja) 2008-09-24
US20030148441A1 (en) 2003-08-07
WO1997007206A1 (en) 1997-02-27

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