DE3177298D1 - Verbesserte vektoren, verfahren zur herstellung solcher vektoren und zur expression von klonierten genen. - Google Patents

Verbesserte vektoren, verfahren zur herstellung solcher vektoren und zur expression von klonierten genen.

Info

Publication number
DE3177298D1
DE3177298D1 DE8181301413T DE3177298T DE3177298D1 DE 3177298 D1 DE3177298 D1 DE 3177298D1 DE 8181301413 T DE8181301413 T DE 8181301413T DE 3177298 T DE3177298 T DE 3177298T DE 3177298 D1 DE3177298 D1 DE 3177298D1
Authority
DE
Germany
Prior art keywords
vectors
producing
cloned genes
improved
expressing cloned
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8181301413T
Other languages
English (en)
Other versions
DE3177298T2 (de
Inventor
Walter Charles Fiers
Erik Rene Remaut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogen Inc
Original Assignee
Biogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27260930&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3177298(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biogen Inc filed Critical Biogen Inc
Application granted granted Critical
Publication of DE3177298D1 publication Critical patent/DE3177298D1/de
Publication of DE3177298T2 publication Critical patent/DE3177298T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/22011—Polyomaviridae, e.g. polyoma, SV40, JC
    • C12N2710/22022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
DE8181301413T 1980-06-06 1981-04-01 Verbesserte vektoren, verfahren zur herstellung solcher vektoren und zur expression von klonierten genen. Expired - Lifetime DE3177298T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB8018701 1980-06-06
GB8026661 1980-08-15
GB8028983 1980-09-08

Publications (2)

Publication Number Publication Date
DE3177298D1 true DE3177298D1 (de) 1993-04-08
DE3177298T2 DE3177298T2 (de) 1993-07-01

Family

ID=27260930

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8181301413T Expired - Lifetime DE3177298T2 (de) 1980-06-06 1981-04-01 Verbesserte vektoren, verfahren zur herstellung solcher vektoren und zur expression von klonierten genen.

Country Status (17)

Country Link
EP (1) EP0041767B1 (de)
JP (6) JPH0787785B2 (de)
KR (1) KR860001557B1 (de)
BG (1) BG60443B2 (de)
BR (1) BR8101972A (de)
CA (1) CA1207251A (de)
DD (1) DD158918A5 (de)
DE (1) DE3177298T2 (de)
DK (1) DK171301B1 (de)
ES (1) ES8302774A1 (de)
FI (1) FI811009L (de)
GR (1) GR74282B (de)
IE (1) IE63126B1 (de)
IL (1) IL62553A (de)
NO (1) NO811119L (de)
PH (1) PH22618A (de)
YU (1) YU87581A (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554513A (en) * 1979-11-21 1996-09-10 Yeda Research & Development Co. Ltd. Production of recombinant human interferon-beta2
US5510472A (en) * 1979-11-21 1996-04-23 Yeda Research And Development Co. Ltd. Production of recombinant human interferon-beta2
IL58765A (en) * 1979-11-21 1986-09-30 Yeda Res & Dev Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
EP0041313B1 (de) 1980-04-03 1990-09-12 Biogen, Inc. DNS-Sequenzen, rekombinante DNS-Moleküle und Verfahren zur Herstellung von dem menschlichen Fibroblast-Interferon
IL65475A0 (en) * 1981-04-17 1982-07-30 Meloy Lab Production of immune interferon and its mrna
EP0068693A3 (de) * 1981-06-16 1983-07-20 Genentech, Inc. Herstellung eines Maul- und Klauenseuche Impfstoffes aus mikrobiellen exprimierten Antigenen
JPS5835198A (ja) * 1981-08-28 1983-03-01 Mitsubishi Petrochem Co Ltd 新規ベクター
ZA831854B (en) * 1982-03-26 1984-01-25 Biogen Nv Small peptides with the specificity of foot and mouth disease viral antigens
EP0090433A1 (de) * 1982-03-31 1983-10-05 Genetics Institute, Inc. Herstellung von modifizierten Proinsulin-Vorläufer codierenden DNA-Sequenzen
FR2526661B1 (fr) * 1982-05-13 1986-02-21 Transgene Sa Nouveaux vecteurs d'expression de la proteine antigenique de la rage et leur application a la preparation de vaccins
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
CA1209501A (en) * 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
AU575301B2 (en) * 1982-10-08 1988-07-28 Juridical Foundation, Japanese Foundation For Cancer Research Novel vector
FR2539758B1 (fr) * 1983-01-21 1985-11-15 Transgene Sa Nouveaux vecteurs d'expression et leur application a la preparation d'une proteine ayant l'activite de l'antitrypsine-a1 humaine
CA1303530C (en) * 1983-01-21 1992-06-16 Michael Courtney EXPRESSION VECTORS AND THEIR USE FOR THE PREPARATION OF A PROTEIN HAVING HUMAN .alpha. -ANTITRYPSIN ACTIVITY
GB8308235D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DE3319242A1 (de) * 1983-05-27 1984-11-29 Battelle-Institut E.V., 6000 Frankfurt Verfahren und plasmid zur steuerung der expression von klonierten genen
US5670371A (en) * 1983-07-15 1997-09-23 Bio-Technology General Corp. Bacterial expression of superoxide dismutase
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
US5162216A (en) * 1983-09-23 1992-11-10 Enzon Labs Inc. Hybrid gene regulatory region operable in E. coli
FR2556365B1 (fr) * 1983-12-09 1987-07-31 Transgene Sa Vecteurs de clonage et d'expression de l'interferon-g, bacteries transformees et procede de preparation de l'interferon-g
US4711845A (en) * 1984-08-31 1987-12-08 Cetus Corporation Portable temperature-sensitive control cassette
EP0172194B1 (de) * 1984-02-08 1994-01-19 Cetus Oncology Corporation Kontrollsysteme für rekombinant-manipulationen
US5081020A (en) * 1984-08-27 1992-01-14 Bio-Technology General Corp. Expression vectors containing λPL promoter, T1 T2 rRNA termination sequence and origin of replication derived from a constitutive high copy number plasmid hosts and related methods
US5059529A (en) * 1984-08-27 1991-10-22 Bio-Technology General Corp. Stabilized expression vectors containing λPL promoter and the gene for the cI434 repressor, plasmids containing the vectors, hosts containing the plasmids and related methods
US5126252A (en) * 1984-08-27 1992-06-30 Bio-Technology General Corp. Expressions vectors containing Lambda PL promoter and T1 T2 RNA termination sequence, plasmids containing the vectors, hosts containing the plasmids and relatedmethods
US5112744A (en) * 1984-08-27 1992-05-12 Bio-Technology General Corp. Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US5162217A (en) * 1984-08-27 1992-11-10 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5759816A (en) * 1984-08-27 1998-06-02 Bio-Technology General Corp. Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
US5143836A (en) * 1984-08-27 1992-09-01 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US5510238A (en) * 1984-10-26 1996-04-23 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Production of human T-cell leukemia (lymphotropic) retrovirus (HTLV-I) envelope protein fragments in bacteria and use in seroepidemiological studies
JP2557053B2 (ja) 1984-12-21 1996-11-27 バイオジェン インコーポレイテッド 腫瘍壊死因子の精製、製造および使用法
US5182196A (en) * 1985-10-09 1993-01-26 Biogen, Inc. Expression systems for overproduction of desired proteins
ATE123526T1 (de) * 1987-08-17 1995-06-15 Hoffmann La Roche Hochreprimierbare expressionskontrollsequenzen.
JP2003204783A (ja) * 1999-07-19 2003-07-22 Ajinomoto Co Inc 発酵法による目的物質の製造法
US7579005B2 (en) 2005-11-28 2009-08-25 E. I. Du Pont De Nemours And Company Process for recombinant expression and purification of antimicrobial peptides using periplasmic targeting signals as precipitable hydrophobic tags

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0041313B1 (de) * 1980-04-03 1990-09-12 Biogen, Inc. DNS-Sequenzen, rekombinante DNS-Moleküle und Verfahren zur Herstellung von dem menschlichen Fibroblast-Interferon
BR8102922A (pt) * 1980-05-12 1982-02-02 Biogen Nv Sequencia de dna, variante de sequencia, molecula de dna recombinante, hospedeiro transformado, polipeptidio ou fragmento ou derivado transformar um hospedeiro, produzir um polipeptidio ou selecionar uma sequencia de dna, bem como composicao e processo para tratar animais fissipedes

Also Published As

Publication number Publication date
EP0041767A2 (de) 1981-12-16
DK148681A (da) 1981-12-07
DK171301B1 (da) 1996-08-26
JP2001136966A (ja) 2001-05-22
YU87581A (en) 1984-04-30
IL62553A (en) 1984-12-31
FI811009L (fi) 1981-12-07
KR860001557B1 (ko) 1986-10-04
JP3158951B2 (ja) 2001-04-23
JPH0838176A (ja) 1996-02-13
JPH0787785B2 (ja) 1995-09-27
JPH10304876A (ja) 1998-11-17
DE3177298T2 (de) 1993-07-01
NO811119L (no) 1981-12-07
JPH0838179A (ja) 1996-02-13
EP0041767B1 (de) 1993-03-03
JPH0838177A (ja) 1996-02-13
IL62553A0 (en) 1981-06-29
BG60443B2 (bg) 1995-03-31
JP3158953B2 (ja) 2001-04-23
EP0041767A3 (en) 1982-09-29
JP3158952B2 (ja) 2001-04-23
KR830005353A (ko) 1983-08-13
ES500967A0 (es) 1983-01-16
IE63126B1 (en) 1995-03-22
GR74282B (de) 1984-06-21
BR8101972A (pt) 1982-08-17
PH22618A (en) 1988-10-28
CA1207251A (en) 1986-07-08
DD158918A5 (de) 1983-02-09
JPS5714599A (en) 1982-01-25
IE810757L (en) 1981-12-06
ES8302774A1 (es) 1983-01-16

Similar Documents

Publication Publication Date Title
DE3177298T2 (de) Verbesserte vektoren, verfahren zur herstellung solcher vektoren und zur expression von klonierten genen.
DE3585953D1 (de) Dna-fragmente, expressionsvektoren, proteine, wirte und verfahren zur herstellung der proteine.
AT374397B (de) Verfahren zur kontinuierlichen herstellung von fuelldraehten, fuelldrahtelektroden od. dgl.
DE3871260D1 (de) Partielles abbauprodukt von proteinen, verfahren zu seiner herstellung und seine verwendung.
AT363469B (de) Verfahren zur herstellung von 3,4,5,6-tetrahydro-2h-1-benzoxocinen und 6a,10a-cis-hexahydrodibenzo(b,d)pyran-9-onen
AT355149B (de) Verfahren zur herstellung von im glanz- bzw. deckvermoegen verbesserten pigmentzusammen- setzungen
AT373885B (de) Verfahren zur herstellung von 1,2,3-thiadiazol -5-yl-harnstoffen
DE3578879D1 (de) Verfahren zur herstellung von 1,3-dimethyl-2-imidazolidinon.
AT376970B (de) Verfahren zur herstellung von 2,3-dihydro-2,2dimethyl-7-hydroxybenzofuran
ATE6255T1 (de) Verfahren zur herstellung von 2,3,5trichlorpyridin.
DE3482971D1 (de) Landwirtschaftliche zusammensetzungen, verfahren zur herstellung von landwirtschaftlichen zusammensetzungen und verfahren zur verwendung von landwirtschaftlichen zusammensetzungen.
ATE11521T1 (de) Verfahren zur herstellung von cyanwasserstoff.
AT367773B (de) Verfahren zur herstellung von 25,26-dihydroxycholesterin
ATA422179A (de) Verfahren zur herstellung von neuen hydrocortison -17,21-diesters
AT371127B (de) Verfahren zur herstellung von vindesin
AT387392B (de) Verfahren zur herstellung von 1alpha, 3betadihydroxy-delta 5-steroiden
ATE86301T1 (de) Verbesserte vektoren, verfahren zur herstellung solcher vektoren und zur expression von klonierten genen.
AT380233B (de) Verfahren zur herstellung von neuen 1,7,7
AT356815B (de) Verfahren zur herstellung von 3',4'-dideoxy- kanamycin b
AT371452B (de) Verfahren zur herstellung von 5-amino-1,2,3 thiadiazolen
ATA263480A (de) Verfahren zur herstellung von 2,2-dialkyl-4-methyl-5,5-dialkyl-3-formyl-3-pyr olinen
ATA482980A (de) Verfahren zur herstellung von 2-oxodihydrobenzo(d)(1,3)-oxazinen
ATE38218T1 (de) Formteil, verbundmaterial und verfahren zu seiner herstellung.
AT356657B (de) Verfahren zur herstellung von 2,6-methano-3- -benzazocinen
AT375083B (de) Verfahren zur herstellung von 1alfa,25-dihydroxycholesterin

Legal Events

Date Code Title Description
8363 Opposition against the patent