DE69530647D1 - Für biosynthetische Insulinvorstufen kodierende DNA-Sequenzen und Verfahren zur Herstellung von Insulin - Google Patents
Für biosynthetische Insulinvorstufen kodierende DNA-Sequenzen und Verfahren zur Herstellung von InsulinInfo
- Publication number
- DE69530647D1 DE69530647D1 DE69530647T DE69530647T DE69530647D1 DE 69530647 D1 DE69530647 D1 DE 69530647D1 DE 69530647 T DE69530647 T DE 69530647T DE 69530647 T DE69530647 T DE 69530647T DE 69530647 D1 DE69530647 D1 DE 69530647D1
- Authority
- DE
- Germany
- Prior art keywords
- insulin
- dna sequences
- precursors
- biosynthetic
- sequences coding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 10
- 102000004877 Insulin Human genes 0.000 title abstract 5
- 108090001061 Insulin Proteins 0.000 title abstract 5
- 229940125396 insulin Drugs 0.000 title abstract 5
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 4
- 239000002243 precursor Substances 0.000 title abstract 3
- 230000001851 biosynthetic effect Effects 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical class OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 abstract 1
- 230000004988 N-glycosylation Effects 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000002538 fungal effect Effects 0.000 abstract 1
- 230000013595 glycosylation Effects 0.000 abstract 1
- 238000006206 glycosylation reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HR940432A HRP940432B1 (en) | 1994-08-05 | 1994-08-05 | Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin |
| HR940432 | 1994-08-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69530647D1 true DE69530647D1 (de) | 2003-06-12 |
| DE69530647T2 DE69530647T2 (de) | 2004-03-11 |
Family
ID=10946142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69530647T Expired - Lifetime DE69530647T2 (de) | 1994-08-05 | 1995-08-03 | Für biosynthetische Insulinvorstufen kodierende DNA-Sequenzen und Verfahren zur Herstellung von Insulin |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0704527B1 (de) |
| CN (1) | CN1126761A (de) |
| AT (1) | ATE239791T1 (de) |
| BG (1) | BG62336B1 (de) |
| CA (1) | CA2155451A1 (de) |
| CZ (1) | CZ199995A3 (de) |
| DE (1) | DE69530647T2 (de) |
| ES (1) | ES2198428T3 (de) |
| HR (1) | HRP940432B1 (de) |
| PL (1) | PL309882A1 (de) |
| PT (1) | PT704527E (de) |
| SI (1) | SI9500250A (de) |
| SK (1) | SK97195A3 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9513967D0 (en) * | 1995-07-08 | 1995-09-06 | Univ Leicester | Insulin |
| WO2000005389A2 (en) * | 1998-07-20 | 2000-02-03 | Unilever N.V. | Production of proteins |
| PT1141014E (pt) | 1999-01-06 | 2005-04-29 | Genentech Inc | Variante mutante do factor de crescimento semelhante a insulina (igf-i) |
| JP3406244B2 (ja) | 1999-04-30 | 2003-05-12 | 伊藤ハム株式会社 | 新規な融合蛋白質からの組み換えインスリンの製造方法 |
| DE19947456A1 (de) | 1999-10-02 | 2001-04-05 | Aventis Pharma Gmbh | C-Peptid zur verbesserten Herstellung von Insulin und Insulinanaloga |
| JP2003528149A (ja) | 2000-03-24 | 2003-09-24 | ジェネンテック・インコーポレーテッド | 軟骨性疾患の治療のためのインスリンの使用 |
| AU2002316807A1 (en) * | 2001-06-08 | 2002-12-23 | Novo Nordisk A/S | Method for making insulin precursors and insulin precursor analogs |
| CA2806399A1 (en) * | 2010-07-28 | 2012-02-02 | Smartcells, Inc. | Recombinantly expressed insulin polypeptides and uses thereof |
| AR087433A1 (es) * | 2011-08-08 | 2014-03-26 | Merck Sharp & Dohme | Analogos de insulina n-glicosilados |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE63757T1 (de) * | 1985-03-28 | 1991-06-15 | Chiron Corp | Expression durch verwendung von fusionsgenen fuer proteinproduktion. |
| FI872102A (fi) * | 1986-06-06 | 1987-12-07 | Panlabs Inc | Expressionssystem hos filamentala fungi. |
| CA1340772C (en) * | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
| DK300090D0 (da) * | 1990-12-19 | 1990-12-19 | Novo Nordisk As | Fremgangsmaade til fremstilling af leadersekvenser |
| BR9400600A (pt) * | 1994-02-17 | 1995-10-24 | Finep Financiadora De Estudos | Processos para produção de uma protéina recombinante e/ou de glicosilfosfatidilinositol (GPI) em células de microorganismos eucariontes e para a obtenção de leveduras de S. cerevisae; célula de levedura sequência de nucleotídios; meio de cultura; medicamento ou vacina; e produto dos ditos processos |
-
1994
- 1994-08-05 HR HR940432A patent/HRP940432B1/xx not_active IP Right Cessation
-
1995
- 1995-08-01 SK SK971-95A patent/SK97195A3/sk unknown
- 1995-08-03 CZ CZ951999A patent/CZ199995A3/cs unknown
- 1995-08-03 ES ES95112210T patent/ES2198428T3/es not_active Expired - Lifetime
- 1995-08-03 DE DE69530647T patent/DE69530647T2/de not_active Expired - Lifetime
- 1995-08-03 EP EP95112210A patent/EP0704527B1/de not_active Expired - Lifetime
- 1995-08-03 BG BG99844A patent/BG62336B1/bg unknown
- 1995-08-03 AT AT95112210T patent/ATE239791T1/de active
- 1995-08-03 PT PT95112210T patent/PT704527E/pt unknown
- 1995-08-04 CA CA002155451A patent/CA2155451A1/en not_active Abandoned
- 1995-08-04 CN CN95115064A patent/CN1126761A/zh active Pending
- 1995-08-04 PL PL95309882A patent/PL309882A1/xx unknown
- 1995-08-04 SI SI9500250A patent/SI9500250A/sl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0704527B1 (de) | 2003-05-07 |
| DE69530647T2 (de) | 2004-03-11 |
| PT704527E (pt) | 2003-09-30 |
| ES2198428T3 (es) | 2004-02-01 |
| BG99844A (bg) | 1996-02-28 |
| SI9500250A (en) | 1996-02-29 |
| SK97195A3 (en) | 1996-02-07 |
| HRP940432B1 (en) | 2003-10-31 |
| PL309882A1 (en) | 1996-02-19 |
| CN1126761A (zh) | 1996-07-17 |
| ATE239791T1 (de) | 2003-05-15 |
| EP0704527A2 (de) | 1996-04-03 |
| CA2155451A1 (en) | 1996-02-06 |
| EP0704527A3 (de) | 1997-08-27 |
| BG62336B1 (bg) | 1999-08-31 |
| CZ199995A3 (en) | 1996-02-14 |
| HRP940432A2 (en) | 1997-08-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |