DE69534930T2 - Molekulares markierungssystem - Google Patents

Molekulares markierungssystem Download PDF

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Publication number
DE69534930T2
DE69534930T2 DE69534930T DE69534930T DE69534930T2 DE 69534930 T2 DE69534930 T2 DE 69534930T2 DE 69534930 T DE69534930 T DE 69534930T DE 69534930 T DE69534930 T DE 69534930T DE 69534930 T2 DE69534930 T2 DE 69534930T2
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Germany
Prior art keywords
polynucleotides
microparticles
oligonucleotide
nucleotides
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69534930T
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German (de)
English (en)
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DE69534930D1 (de
Inventor
Univ. of Cambridge School of Med. S. BRENNER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Solexa Inc
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Solexa Inc
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Publication of DE69534930D1 publication Critical patent/DE69534930D1/de
Publication of DE69534930T2 publication Critical patent/DE69534930T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B80/00—Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00583—Features relative to the processes being carried out
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    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00583—Features relative to the processes being carried out
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    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
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    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
  • Magnetic Heads (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Control Of Driving Devices And Active Controlling Of Vehicle (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Air Transport Of Granular Materials (AREA)
  • Pyrane Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
  • Multiple-Way Valves (AREA)
  • Non-Silver Salt Photosensitive Materials And Non-Silver Salt Photography (AREA)
DE69534930T 1994-10-13 1995-10-12 Molekulares markierungssystem Expired - Lifetime DE69534930T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US358810 1989-05-31
US32234894A 1994-10-13 1994-10-13
US322348 1994-10-13
US08/358,810 US5604097A (en) 1994-10-13 1994-12-19 Methods for sorting polynucleotides using oligonucleotide tags
PCT/US1995/012791 WO1996012014A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Publications (2)

Publication Number Publication Date
DE69534930D1 DE69534930D1 (de) 2006-05-24
DE69534930T2 true DE69534930T2 (de) 2007-01-11

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DE69534930T Expired - Lifetime DE69534930T2 (de) 1994-10-13 1995-10-12 Molekulares markierungssystem

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US (4) US5604097A (enExample)
EP (3) EP0793718B1 (enExample)
JP (6) JP4206130B2 (enExample)
KR (2) KR970707279A (enExample)
AT (1) ATE323159T1 (enExample)
AU (2) AU712929B2 (enExample)
CA (1) CA2202167C (enExample)
CZ (1) CZ86697A3 (enExample)
DE (1) DE69534930T2 (enExample)
FI (1) FI971473L (enExample)
HU (1) HUT77916A (enExample)
NO (1) NO971644L (enExample)
WO (1) WO1996012014A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
DK0834575T3 (da) * 1990-12-06 2002-04-02 Affymetrix Inc A Delaware Corp Identifikation af nucleinsyrer i prøver
US6156501A (en) * 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US7375198B2 (en) * 1993-10-26 2008-05-20 Affymetrix, Inc. Modified nucleic acid probes
US6280935B1 (en) 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6654505B2 (en) 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
EP0832287B1 (en) * 1995-06-07 2007-10-10 Solexa, Inc Oligonucleotide tags for sorting and identification
ATE466109T1 (de) * 1995-06-07 2010-05-15 Solexa Inc Verfahren zur verbesserung der effizienz der polynukleotidsequenzierung
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
EP0880598A4 (en) * 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5948615A (en) * 1996-04-16 1999-09-07 Hitachi, Ltd. Method for analysis of nucleic acid and DNA primer sets for use therein
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US7144119B2 (en) * 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6958245B2 (en) 1996-04-25 2005-10-25 Bioarray Solutions Ltd. Array cytometry
US7041510B2 (en) 1996-04-25 2006-05-09 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
WO1997040385A1 (en) 1996-04-25 1997-10-30 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6048691A (en) * 1996-05-13 2000-04-11 Motorola, Inc. Method and system for performing a binding assay
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
CZ397998A3 (cs) * 1996-06-06 1999-07-14 Lynx Therapeutics, Inc. Způsob sekvenční analýzy ligací kódovaného adaptoru
US7094609B2 (en) 1996-09-20 2006-08-22 Burstein Technologies, Inc. Spatially addressable combinatorial chemical arrays in encoded optical disk format
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
GB9708606D0 (en) * 1997-04-28 1997-06-18 Webster Andrew Sequencing
US6007990A (en) * 1997-04-29 1999-12-28 Levine; Robert A. Detection and quantification of one or more nucleotide sequence target analytes in a sample using spatially localized target analyte replication
JP2002502237A (ja) * 1997-05-12 2002-01-22 ライフ テクノロジーズ，インコーポレイテッド 核酸分子の生成および精製のための方法
EP0985142A4 (en) 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
US6969488B2 (en) * 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
WO1998055657A1 (en) * 1997-06-05 1998-12-10 Cellstore Methods and reagents for indexing and encoding nucleic acids
US6096496A (en) * 1997-06-19 2000-08-01 Frankel; Robert D. Supports incorporating vertical cavity emitting lasers and tracking apparatus for use in combinatorial synthesis
JP2002507126A (ja) * 1997-06-27 2002-03-05 リンクス セラピューティクス，インコーポレイテッド ポリヌクレオチドにおける制限部位をマッピングする方法
AU8409298A (en) * 1997-07-16 1999-02-10 David W. Burden Synthetic oligonucleotide-peptide conjugates and methods of use
US6607878B2 (en) * 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6136537A (en) * 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US6019896A (en) * 1998-03-06 2000-02-01 Molecular Dynamics, Inc. Method for using a quality metric to assess the quality of biochemical separations
EP1066369B1 (en) * 1998-03-18 2006-07-19 Quark Biotech, Inc. Selection subtraction approach to gene identification
AU3572799A (en) * 1998-04-24 1999-11-16 Genova Pharmaceuticals Corporation Function-based gene discovery
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AU4076899A (en) * 1998-05-13 1999-11-29 Spectra Science Corporation Micro-lasing beads and structures, and associated methods
JP3662850B2 (ja) 1998-06-24 2005-06-22 イルミナ インコーポレイテッド 微小球を有するアレイセンサーのデコード
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
CA2346989A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
CA2349836A1 (en) * 1998-11-02 2000-05-11 Lynx Therapeutics, Inc. Method for making complementary oligonucleotide tag sets
CA2352363A1 (en) * 1998-11-27 2000-06-08 Synaptics (Uk) Limited Position sensor
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
CA2372131A1 (en) * 1999-02-22 2000-08-31 Sydney Brenner Polymorphic dna fragments and uses thereof
GB9905807D0 (en) * 1999-03-12 1999-05-05 Amersham Pharm Biotech Uk Ltd Analysis of differential gene expression
AU3519900A (en) * 1999-03-19 2000-10-09 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
JP2002539849A (ja) * 1999-03-26 2002-11-26 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ ユニバーサルアレイ
JP2002540802A (ja) 1999-04-06 2002-12-03 イェール ユニバーシティ 配列標識の固定されたアドレス分析
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6620584B1 (en) 1999-05-20 2003-09-16 Illumina Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) * 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20030186311A1 (en) * 1999-05-21 2003-10-02 Bioforce Nanosciences, Inc. Parallel analysis of molecular interactions
US6573369B2 (en) * 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US20020042081A1 (en) * 2000-10-10 2002-04-11 Eric Henderson Evaluating binding affinities by force stratification and force panning
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20030073250A1 (en) * 1999-05-21 2003-04-17 Eric Henderson Method and apparatus for solid state molecular analysis
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6465183B2 (en) * 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
US7604996B1 (en) 1999-08-18 2009-10-20 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
WO2001014589A2 (en) * 1999-08-20 2001-03-01 Luminex Corporation Liquid array technology
US7211390B2 (en) * 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
AU7586100A (en) * 1999-09-17 2001-04-17 Affymetrix, Inc. Method of cluster analysis of gene expression profiles
US6428957B1 (en) * 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
GB0002389D0 (en) * 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
AU2001238068A1 (en) * 2000-02-07 2001-08-14 Illumina, Inc. Nucleic acid detection methods using universal priming
WO2001057268A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) * 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1257668B1 (en) 2000-02-16 2008-10-29 Illumina, Inc. Parallel genotyping of multiple patient samples
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US6749756B1 (en) * 2000-02-18 2004-06-15 University Of Pittsburgh Reaction and separation methods
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
US7157564B1 (en) 2000-04-06 2007-01-02 Affymetrix, Inc. Tag nucleic acids and probe arrays
US6839562B2 (en) * 2000-04-11 2005-01-04 Telecommunication Systems, Inc. Intelligent delivery agent for short message distribution center
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
EP1349954B1 (en) * 2000-05-19 2011-01-26 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US7230092B2 (en) * 2000-06-06 2007-06-12 Luminex Molecular Diagnostics, Inc. Capture moieties for nucleic acids and uses thereof
JP4744778B2 (ja) * 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド 特定のランダム粒子アレイを適用した複数の被検体分子の分析方法
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
EP1299557B1 (en) 2000-06-30 2007-10-03 Qiagen GmbH Signal amplification with lollipop probes
WO2002014553A2 (en) * 2000-08-11 2002-02-21 Favrille, Inc. A molecular vector identification system
AU8356201A (en) * 2000-08-11 2002-02-25 Agilix Corp Ultra-sensitive detection systems
US6911204B2 (en) 2000-08-11 2005-06-28 Favrille, Inc. Method and composition for altering a B cell mediated pathology
AU2002245009B2 (en) * 2000-08-15 2007-05-17 Bioforce Nanoscience, Inc. Nanoscale molecular arrayer
GB0021367D0 (en) * 2000-09-01 2000-10-18 Sec Dep Of The Home Department Improvements in and relating to marking
US7057704B2 (en) * 2000-09-17 2006-06-06 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
AU1317502A (en) * 2000-10-14 2002-04-29 Eragen Biosciences Inc Solid support assay systems and methods utilizing non-standard bases
US20030045005A1 (en) * 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
EP1366192B8 (en) * 2000-10-24 2008-10-29 The Board of Trustees of the Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
WO2002061133A2 (en) * 2000-11-09 2002-08-08 Yale University Nucleic acid detection using structured probes
CA2428732C (en) * 2000-11-15 2018-07-31 Minerva Biotechnologies Corporation Methods and kit for simultaneous screening of interactions between a plurality of chemical or biological species and uses thereof
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
US20030049616A1 (en) * 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US7226737B2 (en) * 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
US7645868B2 (en) * 2001-01-25 2010-01-12 Luminex Molecular Diagnostics, Inc. Families of non-cross-hybridizing polynucleotides for use as tags and tag complements, manufacture and use thereof
DE60222628T2 (de) * 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
JP2005522980A (ja) * 2001-04-23 2005-08-04 エリトラ ファーマシューティカルズ，インコーポレイテッド アスペルギルス・フミガーツス（Ａｓｐｅｒｇｉｌｌｕｓｆｕｍｉｇａｔｕｓ）の必須遺伝子の同定および使用方法
EP1262563A3 (en) * 2001-05-31 2003-02-05 Takara Bio Inc. Method for immobilizing DNA on a surface
US20070184436A1 (en) * 2001-06-07 2007-08-09 Joel Myerson Generic capture probe arrays
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US20060234231A1 (en) * 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
AU2002329606A1 (en) * 2001-07-17 2003-03-03 Bioforce Nanosciences, Inc. Combined molecular blinding detection through force microscopy and mass spectrometry
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US7026123B1 (en) * 2001-08-29 2006-04-11 Pioneer Hi-Bred International, Inc. UTR tag assay for gene function discovery
US7042488B2 (en) 2001-09-27 2006-05-09 Fujinon Corporation Electronic endoscope for highlighting blood vessel
US6767733B1 (en) 2001-10-10 2004-07-27 Pritest, Inc. Portable biosensor apparatus with controlled flow
US20030148335A1 (en) * 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
IL161391A0 (en) 2001-10-15 2004-09-27 Bioarray Solutions Ltd Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
US7132236B2 (en) * 2001-10-25 2006-11-07 Agilent Technologies, Inc. Composition and method for optimized hybridization using modified solutions
WO2003038091A1 (en) * 2001-10-29 2003-05-08 Japan Science And Technology Agency Oligonucleotide sequences free from mishybridization and method of designing the same
WO2003056298A2 (en) * 2001-11-06 2003-07-10 Agilix Corporation Sensitive coded detection systems
EP1314783B1 (de) * 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US20040020993A1 (en) * 2001-12-28 2004-02-05 Green Larry R. Method for luminescent identification and calibration
US7413854B2 (en) * 2002-03-15 2008-08-19 Nuevolution A/S Method for synthesising templated molecules
ATE423224T1 (de) * 2002-04-26 2009-03-15 Solexa Inc Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden
US20040060987A1 (en) * 2002-05-07 2004-04-01 Green Larry R. Digital image analysis method for enhanced and optimized signals in fluorophore detection
US20050239193A1 (en) * 2002-05-30 2005-10-27 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of microorganisms and microparticles
AU2003243720A1 (en) * 2002-06-21 2004-01-06 Bayer Bioscience N.V. Method for detecting foreign dna in a host genome
US20050186573A1 (en) * 2002-07-24 2005-08-25 Janeczko Richard A. Polynucleotides for use as tags and tag complements in the detection of nucleic acid sequences
AU2003247266A1 (en) * 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US20040043384A1 (en) * 2002-08-28 2004-03-04 Oleinikov Andrew V. In vitro protein translation microarray device
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
AU2003266932B2 (en) * 2002-09-27 2009-04-09 Mpm-Holding Aps Spatially encoded polymer matrix
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
SI3299463T1 (sl) 2002-10-30 2021-03-31 Nuevolution A/S Encimsko kodiranje
US20040086892A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
EP1583845A4 (en) * 2003-01-02 2006-06-28 Bioforce Nanosciences Inc METHOD AND APPARATUS FOR MOLECULAR ANALYSIS IN SMALL SAMPLE VOLUMES
DE602004018270D1 (de) * 2003-01-17 2009-01-22 Eragen Biosciences Inc Nukleinsäureamplifikation mit nichtstandardbasen
WO2004065000A1 (en) * 2003-01-21 2004-08-05 Illumina Inc. Chemical reaction monitor
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CN102212614B (zh) * 2003-01-29 2013-12-25 454生命科学公司 核酸扩增和测序的方法
US20060134624A1 (en) * 2003-02-06 2006-06-22 Salerno John C Polymerase-based protocols for the introductions of deletions and insertions
US20060257876A1 (en) * 2003-02-06 2006-11-16 Rensselaer Polytechnis Institute Polymerase-based protocols for generating chimeric and combinatorial...
US20060228786A1 (en) * 2003-02-06 2006-10-12 Salerno John C Polymerase-based protocols for the introduction of deletions and insertions
WO2004072247A2 (en) * 2003-02-06 2004-08-26 Rensselaer Polytechnic Institute Polymerase-based protocols for generating chimeric and combinatorial chimeric oligonucleotides
US20070026397A1 (en) * 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
EP1597394A2 (en) * 2003-02-21 2005-11-23 Nuevolution A/S A method for obtaining structural information about an encoded molecule
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
DE602004019764D1 (de) 2003-03-20 2009-04-16 Nuevolution As Ligationsvermittelnde codierung von kleinen molekülen
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
JP4567673B2 (ja) 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
US7195875B2 (en) * 2003-04-18 2007-03-27 Beckman Coulter, Inc. Oligonucleotide pairs for multiplexed binding assays
JP2004355294A (ja) * 2003-05-29 2004-12-16 National Institute Of Advanced Industrial & Technology 情報担体としてのｄｎａ符号の設計方法
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
WO2005010494A2 (en) * 2003-07-21 2005-02-03 Jie Wu Multiplexed analyte detection
US7365179B2 (en) * 2003-09-09 2008-04-29 Compass Genetics, Llc Multiplexed analytical platform
US20050100939A1 (en) * 2003-09-18 2005-05-12 Eugeni Namsaraev System and methods for enhancing signal-to-noise ratios of microarray-based measurements
US7927796B2 (en) * 2003-09-18 2011-04-19 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
ATE447626T1 (de) 2003-09-18 2009-11-15 Nuevolution As Methode zur gewinnung struktureller informationen kodierter moleküle und zur selektion von verbindungen
NZ546072A (en) * 2003-09-22 2009-08-28 Bioarray Solutions Ltd Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US20050089916A1 (en) * 2003-10-28 2005-04-28 Xiongwu Xia Allele assignment and probe selection in multiplexed assays of polymorphic targets
NZ547492A (en) 2003-10-28 2009-12-24 Bioarray Solutions Ltd Optimization of gene expression analysis using immobilized capture probes of different lengths and densities
WO2005045060A2 (en) 2003-10-29 2005-05-19 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
US20050094807A1 (en) * 2003-11-04 2005-05-05 John Silzel Accuracy array assay system and method
AU2004290036B2 (en) * 2003-11-10 2011-08-04 Geneohm Sciences, Inc. Nucleic acid detection method having increased sensitivity
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP3673986A1 (en) 2004-01-07 2020-07-01 Illumina Cambridge Limited Improvements in or relating to molecular arrays
WO2005071110A2 (en) * 2004-01-23 2005-08-04 Lingvitae As Improving polynucleotide ligation reactions
DE602004029326D1 (de) 2004-01-23 2010-11-11 Sloning Biotechnology Gmbh Enzymatische Herstellung von Nukleinsäuremolekülen
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
GB0402895D0 (en) * 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
US20080108515A1 (en) * 2004-02-10 2008-05-08 Gormley Niall A Arrayed polynucleotides
CA2555377A1 (en) * 2004-02-12 2005-09-01 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
US20090239211A1 (en) * 2004-02-17 2009-09-24 Nuevolution A/S Method For Enrichment Involving Elimination By Mismatch Hybridisation
EP2248911A1 (en) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
WO2005090566A2 (en) 2004-03-22 2005-09-29 Nuevolution A/S Ligational encoding using building block oligonucleotides
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
EP1582599A1 (en) * 2004-03-31 2005-10-05 Takara Bio Inc. Method for purifying microbeads
US20050266447A1 (en) * 2004-04-19 2005-12-01 Pioneer Hi-Bred International, Inc. Method for identifying activators of gene transcription
US20050250147A1 (en) * 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
EP1766090B1 (en) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
EP1771563A2 (en) 2004-05-28 2007-04-11 Ambion, Inc. METHODS AND COMPOSITIONS INVOLVING MicroRNA
US7363170B2 (en) * 2004-07-09 2008-04-22 Bio Array Solutions Ltd. Transfusion registry network providing real-time interaction between users and providers of genetically characterized blood products
WO2006014869A1 (en) * 2004-07-26 2006-02-09 Parallele Bioscience, Inc. Simultaneous analysis of multiple genomes
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP1623996A1 (en) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved method of selecting a desired protein from a library
WO2006034387A1 (en) 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006047791A2 (en) * 2004-10-27 2006-05-04 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library device and method for use
EP1838852A2 (en) 2004-11-12 2007-10-03 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
DE502005003509D1 (de) * 2004-11-19 2008-05-08 Ebm Papst St Georgen Gmbh & Co Anordnung mit einem luefter und einer pumpe
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP2230315A1 (en) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
EP2233583A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
EP1851551A2 (en) * 2005-02-03 2007-11-07 Perkinelmer Las, Inc. Ultra-sensitive detection systems using multidimension signals
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060204971A1 (en) * 2005-03-11 2006-09-14 Varde Shobha A Oligonucleotides for multiplexed binding assays
US20060211030A1 (en) * 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
US7498136B2 (en) * 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
WO2006133184A2 (en) 2005-06-07 2006-12-14 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
CN101466847B (zh) 2005-06-15 2014-02-19 考利达基因组股份有限公司 用于遗传和化学分析的单分子阵列
WO2007025594A1 (en) * 2005-07-07 2007-03-08 Pamgene Bv Method for detection and quantification of target nucleic acids in a sample
US20070172429A1 (en) * 2005-08-12 2007-07-26 Xiaolian Gao Labeling compositions and methods of use for deterrent trackability
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
SI2336315T1 (sl) * 2005-12-01 2017-12-29 Nuevolution A/S Postopki encimskega kodiranja za učinkovito sintezo velikih knjižnic
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007087310A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007087312A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
US20090062129A1 (en) * 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US7910695B2 (en) * 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7888465B2 (en) * 2006-06-29 2011-02-15 The Invention Science Fund, I, LLC Methods for arbitrary peptide synthesis
US7777002B2 (en) * 2006-06-29 2010-08-17 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
US20090002703A1 (en) * 2006-08-16 2009-01-01 Craig Edward Parman Methods and systems for quantifying isobaric labels and peptides
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
EP2115138A2 (en) 2006-09-19 2009-11-11 Asuragen, Inc. Micrornas differentially expressed in pancreatic diseases and uses thereof
AU2007299748A1 (en) 2006-09-19 2008-03-27 Asuragen, Inc. miR-15, miR-26, miR -31,miR -145, miR-147, miR-188, miR-215, miR-216 miR-331, mmu-miR-292-3p regulated genes and pathways as targets for therapeutic intervention
EP2099930B1 (en) 2006-12-13 2015-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
AR066922A1 (es) 2007-06-08 2009-09-23 Monsanto Technology Llc Metodos de mejoramiento molecular del germoplasma de una planta por secuenciamiento dirigido
US7635566B2 (en) * 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
WO2009040682A2 (en) * 2007-09-26 2009-04-02 Population Genetics Technologies Ltd. Methods and compositions for reducing the complexity of a nucleic acid sample
US7958066B2 (en) * 2007-11-02 2011-06-07 Hunch Inc. Interactive machine learning advice facility
EP2215245A2 (en) * 2007-11-02 2010-08-11 Luminex Molecular Diagnostics, Inc. One-step target detection assay
US8815576B2 (en) * 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
EP2285960B1 (en) 2008-05-08 2015-07-08 Asuragen, INC. Compositions and methods related to mir-184 modulation of neovascularization or angiogenesis
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
EP4335932A3 (en) 2008-11-07 2024-06-26 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
PT2387627E (pt) 2009-01-15 2016-06-03 Adaptive Biotechnologies Corp Determinação do perfil de imunidade adaptativa e métodos de geração de anticorpos monoclonais
KR102231531B1 (ko) 2009-02-13 2021-03-23 엑스-켐, 인크. Ｄｎａ―코딩된 라이브러리의 생성 및 스크리닝 방법
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
CN104404134B (zh) 2009-04-03 2017-05-10 莱弗斯基因股份有限公司 多重核酸检测方法和系统
WO2010126614A2 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
CN102686728B (zh) 2009-06-29 2018-09-18 卢米耐克斯公司 具有发夹构象的嵌合引物及其使用方法
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
US10385391B2 (en) 2009-09-22 2019-08-20 President And Fellows Of Harvard College Entangled mate sequencing
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2491146B1 (en) * 2009-10-23 2017-09-20 Luminex Corporation Amplification primers with non-standard bases for increased reaction specificity
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
ES2631458T3 (es) 2010-03-04 2017-08-31 Interna Technologies B.V. Molécula de ARNmi definida por su fuente y sus usos terapéuticos en el cáncer asociado a la EMT
EP3540059A1 (en) 2010-04-16 2019-09-18 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
US8278049B2 (en) * 2010-04-26 2012-10-02 Ann & Robert H. Lurie Children's Hospital of Chicago Selective enrichment of CpG islands
EP4219759A3 (en) 2010-05-06 2023-09-06 Adaptive Biotechnologies Corporation Monitoring health and disease status using clonotype profiles
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP3369817A1 (en) 2010-07-06 2018-09-05 InteRNA Technologies B.V. Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma , or in diseases or conditions with activated braf pathway
JP5992911B2 (ja) 2010-09-21 2016-09-14 ポピュレーション ジェネティクス テクノロジーズ リミテッド 分子計数による対立遺伝子呼び出しの信頼度の増加
IN2013MN00522A (enExample) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
WO2012050920A1 (en) 2010-09-29 2012-04-19 Illumina, Inc. Compositions and methods for sequencing nucleic acids
EP2622103B2 (en) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
EP3000883B8 (en) 2010-11-12 2018-02-28 Gen9, Inc. Methods and devices for nucleic acids synthesis
ES2629890T3 (es) 2010-11-17 2017-08-16 Interpace Diagnostics, Llc miARN como biomarcadores para distinguir entre neoplasias de tiroides benignas y malignas
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US20140057295A1 (en) 2011-02-28 2014-02-27 Barbara J. Stegmann Anti-mullerian hormone changes in pregnancy and prediction of adverse pregnancy outcomes and gender
US10501779B2 (en) 2011-05-12 2019-12-10 President And Fellows Of Harvard College Oligonucleotide trapping
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
EP3517611A3 (en) * 2011-06-15 2019-09-04 Gen9, Inc. Methods for preparative in vitro cloning
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
ES2737957T3 (es) 2011-08-26 2020-01-17 Gen9 Inc Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos
EA032438B1 (ru) 2011-09-07 2019-05-31 Икс-Чем, Инк. Способы мечения днк-кодированных библиотек
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
CN108192952A (zh) 2011-09-29 2018-06-22 露美内克丝公司 水解探针
US9228233B2 (en) 2011-10-17 2016-01-05 Good Start Genetics, Inc. Analysis methods
US9279159B2 (en) 2011-10-21 2016-03-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
WO2013063519A1 (en) 2011-10-26 2013-05-02 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
EP2771487A1 (en) 2011-10-27 2014-09-03 Asuragen, INC. Mirnas as diagnostic biomarkers to distinguish benign from malignant thyroid tumors
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013096802A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Amplification primers and methods
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
EP2820158B1 (en) 2012-02-27 2018-01-10 Cellular Research, Inc. Compositions and kits for molecular counting
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP2820155B1 (en) 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP4001427A1 (en) 2012-04-24 2022-05-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
EP2831276B1 (en) 2012-05-08 2016-04-20 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US9663818B2 (en) 2012-06-15 2017-05-30 The University Of Chicago Oligonucleotide-mediated quantitative multiplexed immunoassays
EP3483311B8 (en) 2012-06-25 2025-10-29 Ginkgo Bioworks, Inc. Methods for nucleic acid assembly and high throughput sequencing
US20150152499A1 (en) 2012-07-03 2015-06-04 Interna Technologies B.V. Diagnostic portfolio and its uses
EA201992285A1 (ru) 2012-07-13 2020-05-31 Икс-Чем, Инк. Днк-кодированные библиотеки, содержащие связи кодирующих олигонуклеотидов, не доступные для считывания полимеразами
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
DK2904111T3 (en) 2012-10-01 2018-03-12 Adaptive Biotechnologies Corp Immune competence evaluation of adaptive immune receptor diversity and clonality characterization
WO2014055117A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
US9365896B2 (en) 2012-10-19 2016-06-14 Agilent Technologies, Inc. Addition of an adaptor by invasive cleavage
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
US10597710B2 (en) 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US10597650B2 (en) 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
US9593373B2 (en) 2013-03-15 2017-03-14 Illumina Cambridge Limited Modified nucleosides or nucleotides
CA2907377A1 (en) 2013-03-15 2014-09-18 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
EP3404116B1 (en) 2013-03-15 2022-10-19 The University of Chicago Methods and compositions related to t-cell activity
US9540685B2 (en) 2013-03-15 2017-01-10 President And Fellows Of Harvard College Methods of identifying homologous genes using FISH
EP3366785A3 (en) 2013-03-15 2018-09-19 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
WO2014165814A1 (en) 2013-04-04 2014-10-09 Georgia State University Research Foundation, Inc. Rna microchip detection using nanoparticle-assisted signal amplification
WO2014172288A2 (en) 2013-04-19 2014-10-23 Raindance Technologies, Inc. Digital analyte analysis
CN111394426B (zh) 2013-05-23 2024-05-10 斯坦福大学托管董事会 用于个人表观基因组学的至天然染色质的转座
WO2014197377A2 (en) 2013-06-03 2014-12-11 Good Start Genetics, Inc. Methods and systems for storing sequence read data
EP3011056B1 (en) 2013-06-19 2019-03-06 Luminex Corporation Real-time multiplexed hydrolysis probe assay
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
CN107435067B (zh) 2013-08-09 2021-07-09 卢米耐克斯公司 用于在核酸测定中改善熔链分辨和多重性的探针
WO2015026845A2 (en) 2013-08-19 2015-02-26 Abbott Molecular Inc. Nucleotide analogs
ES2873850T3 (es) 2013-08-19 2021-11-04 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
JP6545682B2 (ja) 2013-08-28 2019-07-17 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 大規模並列単一細胞分析
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3055676A1 (en) 2013-10-07 2016-08-17 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
CN106170558B (zh) 2013-10-21 2021-10-29 金圣千 利用寡核苷酸的生物分子的分析方法及装置
EP3062252A4 (en) 2013-10-22 2017-06-07 KIM, Sung-Chun Marker for generating binding information on biomolecules and nucleic acids, preparation method therefor, and method and apparatus for analyzing biomolecule by using same
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
SG11201604923XA (en) 2013-12-28 2016-07-28 Guardant Health Inc Methods and systems for detecting genetic variants
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
US10435738B2 (en) 2014-01-10 2019-10-08 Kyoto University RNA microarray for detecting interaction between protein and RNA containing a higher-order structure
EP3114240B1 (en) 2014-03-05 2019-07-24 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
EP3967771B1 (en) 2014-08-11 2023-10-04 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US10093967B2 (en) 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
US10174383B2 (en) 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
CN107075546B (zh) 2014-08-19 2021-08-31 哈佛学院董事及会员团体 用于对核酸探测并作图的rna-引导的系统
EP3842544A1 (en) * 2014-09-05 2021-06-30 QIAGEN GmbH Preparation of adapter-ligated amplicons
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
JP2017530720A (ja) 2014-10-17 2017-10-19 グッド スタート ジェネティクス， インコーポレイテッド 着床前遺伝子スクリーニングおよび異数性検出
EP3212790B1 (en) 2014-10-29 2020-03-25 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
AU2015353581A1 (en) 2014-11-25 2017-06-15 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US10066259B2 (en) 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
EP3766988B1 (en) 2015-02-19 2024-02-14 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
US10246702B1 (en) 2015-02-23 2019-04-02 New England Biolabs, Inc. Compositions and methods for labeling target nucleic acid molecules
WO2016138122A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP3277843A2 (en) 2015-03-30 2018-02-07 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
ES2963242T3 (es) 2015-09-18 2024-03-25 Vanadis Diagnostics Conjunto de sondas para analizar una muestra de ADN y método para utilizar el mismo
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
CN108603228B (zh) 2015-12-17 2023-09-01 夸登特健康公司 通过分析无细胞dna确定肿瘤基因拷贝数的方法
US9931638B2 (en) 2016-02-24 2018-04-03 Latonya Stewart Garbage disposal cleaning system
WO2017168331A1 (en) 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex fragment sequencing
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
EP4269616A3 (en) 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10240196B2 (en) 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3500669A4 (en) 2016-08-16 2020-01-22 The Regents of the University of California METHOD FOR SEARCHING FOR LOW ABUNDANCE SEQUENCES BY HYBRIDIZATION (FLASH)
EP4219746B1 (en) 2016-09-02 2024-12-18 New England Biolabs, Inc. Analysis of chromatin using a nicking enzyme
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10487358B2 (en) 2016-09-23 2019-11-26 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP3529400B1 (en) 2016-10-24 2021-02-17 Geneinfosec, Inc. Concealing information present within nucleic acids
WO2018081604A1 (en) 2016-10-28 2018-05-03 Grail, Inc. Methods for single-stranded nucleic acid library preparation
US11608497B2 (en) 2016-11-08 2023-03-21 Becton, Dickinson And Company Methods for cell label classification
WO2018089378A1 (en) 2016-11-08 2018-05-17 Cellular Research, Inc. Methods for expression profile classification
WO2018104908A2 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
US20180163201A1 (en) 2016-12-12 2018-06-14 Grail, Inc. Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
WO2018119399A1 (en) 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
WO2018132610A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
JP7145517B2 (ja) 2017-03-08 2022-10-03 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 分析物の検出
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
EP3630790B1 (en) * 2017-05-23 2023-05-10 Rutgers, The State University of New Jersey Target mediatedin situ
AU2018281745B2 (en) 2017-06-05 2022-05-19 Becton, Dickinson And Company Sample indexing for single cells
EP3638786B1 (en) 2017-06-15 2025-04-16 Genome Research Limited Duplex sequencing using direct repeat molecules
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
US20190076814A1 (en) 2017-09-11 2019-03-14 Synthego Corporation Biopolymer synthesis system and method
AU2018358016A1 (en) 2017-11-03 2020-05-07 Interna Technologies B.V. MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
WO2019104155A2 (en) 2017-11-22 2019-05-31 The University Of Chicago Chemical probe-dependent evaluation of protein activity and uses thereof
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US11414656B2 (en) 2017-12-15 2022-08-16 Grail, Inc. Methods for enriching for duplex reads in sequencing and error correction
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
WO2019126803A1 (en) 2017-12-22 2019-06-27 Grail, Inc. Error removal using improved library preparation methods
GB2589159B (en) 2017-12-29 2023-04-05 Clear Labs Inc Nucleic acid sequencing apparatus
EP3553182A1 (en) 2018-04-11 2019-10-16 Université de Bourgogne Detection method of somatic genetic anomalies, combination of capture probes and kit of detection
ES3014208T3 (en) 2018-05-03 2025-04-21 Becton Dickinson Co Molecular barcoding on opposite transcript ends
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
KR102209178B1 (ko) * 2018-07-17 2021-01-29 이윤경 유전체 및 유전체 정보의 보존 및 활용을 위한 방법
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
KR20210104779A (ko) 2018-12-13 2021-08-25 디엔에이 스크립트 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성
KR20210111262A (ko) 2019-01-03 2021-09-10 디엔에이 스크립트 올리고뉴클레오티드 세트의 원 포트 합성
DK3884071T3 (da) 2019-01-03 2025-06-10 Ncan Genomics Inc Sammenkædet måloptagelse
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
CN113574178B (zh) 2019-01-23 2024-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
ES2953889T3 (es) 2019-03-14 2023-11-16 Genome Res Ltd Método para secuenciar una repetición directa
US20220205035A1 (en) 2019-04-05 2022-06-30 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
US12441716B2 (en) 2019-04-12 2025-10-14 The Regents Of The University Of California Compositions and methods for increasing muscle mass and oxidative metabolism
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
ES2987640T3 (es) 2019-11-08 2024-11-15 Becton Dickinson Co Uso de cebado aleatorio para obtener información de V(D)J de longitud completa para la secuenciación del repertorio inmunitario
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
CN115335520A (zh) 2020-01-29 2022-11-11 贝克顿迪金森公司 用于通过测序对单细胞进行空间映射的条形码化的孔
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2023012521A1 (en) 2021-08-05 2023-02-09 Inivata Limited Highly sensitive method for detecting cancer dna in a sample
WO2022029688A1 (en) 2020-08-05 2022-02-10 Inivata Ltd. Highly sensitive method for detecting cancer dna in a sample
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
CN118266034A (zh) 2021-10-04 2024-06-28 F·霍夫曼-罗氏股份公司 在线碱基识别压缩
EP4547839A1 (en) 2022-06-30 2025-05-07 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
WO2024028794A1 (en) 2022-08-02 2024-02-08 Temple Therapeutics BV Methods for treating endometrial and ovarian hyperproliferative disorders
WO2025104680A1 (en) 2023-11-17 2025-05-22 Friedrich Miescher Institute For Biomedical Research Dna accessibility for the assessment of male fertility
WO2025158200A1 (en) 2024-01-25 2025-07-31 Pixelgen Technologies Ab Method for spatial mapping of cell targets
WO2025212586A1 (en) 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads
WO2025248505A1 (en) 2024-05-31 2025-12-04 Wayne State University Methods for treating endometrial and ovarian hyperproliferative disorders

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4046720A (en) 1974-01-17 1977-09-06 California Institute Of Technology Crosslinked, porous, polyacrylate beads
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4413070A (en) 1981-03-30 1983-11-01 California Institute Of Technology Polyacrolein microspheres
US4678814A (en) 1981-03-30 1987-07-07 California Institute Of Technology Polyacrolein microspheres
US4948882A (en) 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4659774A (en) 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
US5206143A (en) 1985-11-01 1993-04-27 Smithkline Beecham Corporation Method and reagents for performing subset analysis using quantitative differences in fluorescence intensity
US5093232A (en) 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4855225A (en) 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5091519A (en) 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
SE458968B (sv) 1987-06-16 1989-05-22 Wallac Oy Biospecifikt analysfoerfarande foer flera analyter i vilket ingaar partikelraekning och maerkning med fluorescerande maerksubstanser
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
JP2597698B2 (ja) 1987-10-28 1997-04-09 ハワード・フローレイ・インスティテュト・オブ・イクスペリメンタル・フィジオロジー・アンド・メディシン オリゴヌクレオチド‐ポリアミド コンジュゲート
US5104791A (en) 1988-02-09 1992-04-14 E. I. Du Pont De Nemours And Company Particle counting nucleic acid hybridization assays
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
JPH05501709A (ja) 1989-11-17 1993-04-02 リンクス　セラピューティクス，インコーポレイテッド ポリ（アルキルおよびアルケニルホスフェート）およびそれらのチオホスフェートおよび抗ウイルス剤としてのセレノホスフェート誘導体
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5650489A (en) * 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
ATE174598T1 (de) * 1990-08-24 1999-01-15 Ixsys Inc Verfahren zur herstellung von oligonukleotiden mit regellosen codonen
EP0687691B1 (en) 1990-08-31 1998-10-07 Regents Of The University Of Minnesota Resin for solid-phase peptide synthesis
DK0834575T3 (da) * 1990-12-06 2002-04-02 Affymetrix Inc A Delaware Corp Identifikation af nucleinsyrer i prøver
JPH057490A (ja) * 1991-05-25 1993-01-19 Sumitomo Electric Ind Ltd Ｄｎａ断片の分取法
WO1993002360A1 (en) 1991-07-16 1993-02-04 Transmed Biotech Incorporated Methods and compositions for simultaneous analysis of multiple analytes
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
EP0773227A1 (en) * 1991-09-18 1997-05-14 Affymax Technologies N.V. Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides
AU2657392A (en) 1991-09-27 1993-04-27 Allelix Biopharmaceuticals Inc. Duplex-forming, polynucleotide conjugates
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
AU679903B2 (en) * 1991-12-24 1997-07-17 Tepnel Medical Limited Manipulating nucleic acid sequences
EP1382386A3 (en) * 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
JP4138869B2 (ja) * 1992-04-15 2008-08-27 ザ・ジョーンズ・ホプキンス・ユニバーシティ 多様性のある有用なオリゴヌクレオチド集合体の合成
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5541061A (en) * 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
JP3311752B2 (ja) 1992-07-02 2002-08-05 ソイニ，エルッキ 生体特異的多変数検定法
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
AU4779493A (en) * 1992-07-21 1994-02-14 Bunsen Rush Laboratories Inc. Oligomer library formats and methods relating thereto
HUT72495A (en) * 1992-10-01 1996-05-28 Cold Spring Harbor Lab Complex combinatorial chemical libraries encoded with tags
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
DE69535428T2 (de) 1994-02-14 2007-12-06 Smithkline Beecham Corp. Verfahren zum Auffinden von differentiel exprimierte Gene
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage

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