CA2202167C - Molecular tagging system - Google Patents

Molecular tagging system Download PDF

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Publication number
CA2202167C
CA2202167C CA002202167A CA2202167A CA2202167C CA 2202167 C CA2202167 C CA 2202167C CA 002202167 A CA002202167 A CA 002202167A CA 2202167 A CA2202167 A CA 2202167A CA 2202167 C CA2202167 C CA 2202167C
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CA
Canada
Prior art keywords
polynucleotides
oligonucleotide
tag
nucleotides
microparticles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002202167A
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English (en)
French (fr)
Other versions
CA2202167A1 (en
Inventor
Sydney Brenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lynx Therapeutics Inc
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Lynx Therapeutics Inc
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Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Priority to CA002441634A priority Critical patent/CA2441634A1/en
Publication of CA2202167A1 publication Critical patent/CA2202167A1/en
Application granted granted Critical
Publication of CA2202167C publication Critical patent/CA2202167C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/09—Recombinant DNA-technology
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    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B80/00—Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers
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    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00718—Type of compounds synthesised
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  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Control Of Driving Devices And Active Controlling Of Vehicle (AREA)
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  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Air Transport Of Granular Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
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CA002202167A 1994-10-13 1995-10-12 Molecular tagging system Expired - Fee Related CA2202167C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002441634A CA2441634A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US32234894A 1994-10-13 1994-10-13
US08/322,348 1994-10-13
US08/358,810 1994-12-19
US08/358,810 US5604097A (en) 1994-10-13 1994-12-19 Methods for sorting polynucleotides using oligonucleotide tags
PCT/US1995/012791 WO1996012014A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002441634A Division CA2441634A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Publications (2)

Publication Number Publication Date
CA2202167A1 CA2202167A1 (en) 1996-04-25
CA2202167C true CA2202167C (en) 2003-12-16

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CA002202167A Expired - Fee Related CA2202167C (en) 1994-10-13 1995-10-12 Molecular tagging system

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US (4) US5604097A (enExample)
EP (3) EP0793718B1 (enExample)
JP (6) JP4206130B2 (enExample)
KR (2) KR970707279A (enExample)
AT (1) ATE323159T1 (enExample)
AU (2) AU712929B2 (enExample)
CA (1) CA2202167C (enExample)
CZ (1) CZ86697A3 (enExample)
DE (1) DE69534930T2 (enExample)
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EP1724348A3 (en) 2008-10-08
JPH10507357A (ja) 1998-07-21
AU784741B2 (en) 2006-06-08
AU4277896A (en) 1996-05-06
FI971473A0 (fi) 1997-04-09
KR19990022543A (ko) 1999-03-25
CZ86697A3 (en) 1997-09-17
JP4712822B2 (ja) 2011-06-29
US5604097A (en) 1997-02-18
FI971473A7 (fi) 1997-06-04
JP2008301806A (ja) 2008-12-18
JP2005052146A (ja) 2005-03-03
JP4206130B2 (ja) 2009-01-07
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EP0793718B1 (en) 2006-04-12
WO1996012014A1 (en) 1996-04-25

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