DE69534389D1 - Mit PNA- und Amidat-Etiketten bestückte Festphasenträger - Google Patents

Mit PNA- und Amidat-Etiketten bestückte Festphasenträger

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Publication number
DE69534389D1
DE69534389D1 DE69534389T DE69534389T DE69534389D1 DE 69534389 D1 DE69534389 D1 DE 69534389D1 DE 69534389 T DE69534389 T DE 69534389T DE 69534389 T DE69534389 T DE 69534389T DE 69534389 D1 DE69534389 D1 DE 69534389D1
Authority
DE
Germany
Prior art keywords
amidate
pna
labels
solid phase
phase carriers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69534389T
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English (en)
Other versions
DE69534389T2 (de
Inventor
Sydney Brenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Solexa Inc
Original Assignee
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Publication of DE69534389D1 publication Critical patent/DE69534389D1/de
Application granted granted Critical
Publication of DE69534389T2 publication Critical patent/DE69534389T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/6869—Methods for sequencing
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DE69534389T 1994-10-13 1995-10-12 Mit PNA- und Amidat-Etiketten bestückte Festphasenträger Expired - Lifetime DE69534389T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32234894A 1994-10-13 1994-10-13
US322348 1994-10-13
US359295 1994-12-19
US08/359,295 US5695934A (en) 1994-10-13 1994-12-19 Massively parallel sequencing of sorted polynucleotides

Publications (2)

Publication Number Publication Date
DE69534389D1 true DE69534389D1 (de) 2005-09-22
DE69534389T2 DE69534389T2 (de) 2006-06-29

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DE69534389T Expired - Lifetime DE69534389T2 (de) 1994-10-13 1995-10-12 Mit PNA- und Amidat-Etiketten bestückte Festphasenträger
DE69513997T Expired - Lifetime DE69513997T2 (de) 1994-10-13 1995-10-12 Massive parallelsequenzierung an sortierten polynucleotiden

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DE69513997T Expired - Lifetime DE69513997T2 (de) 1994-10-13 1995-10-12 Massive parallelsequenzierung an sortierten polynucleotiden

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US (3) US5695934A (de)
EP (2) EP0786014B1 (de)
AU (1) AU3946195A (de)
DE (2) DE69534389T2 (de)
WO (1) WO1996012039A1 (de)

Families Citing this family (422)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
EP0562047A4 (en) * 1990-12-06 1995-11-02 Affymax Tech Nv Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
USRE43097E1 (en) * 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6280935B1 (en) 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
WO1996041011A1 (en) * 1995-06-07 1996-12-19 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
ATE466109T1 (de) * 1995-06-07 2010-05-15 Solexa Inc Verfahren zur verbesserung der effizienz der polynukleotidsequenzierung
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US6312893B1 (en) * 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) * 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (de) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Erkennung von Nukleinsäuresequenz-Unterschieden mittels der gleichzeitigen Ligaseerkennungs- und Polymerasekettenreaktion
GB9618544D0 (en) * 1996-09-05 1996-10-16 Brax Genomics Ltd Characterising DNA
WO1998012559A1 (en) 1996-09-20 1998-03-26 Demers James P Spatially addressable combinatorial chemical arrays in cd-rom format
US5935793A (en) * 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US6124092A (en) * 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
JP3935509B2 (ja) 1997-02-12 2007-06-27 ワイ． チャン，ユージーン ポリマー分析のための方法および製品
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
JP4294740B2 (ja) * 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
AU739963B2 (en) 1997-06-27 2001-10-25 Lynx Therapeutics, Inc. Method of mapping restriction sites in polynucleotides
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6607878B2 (en) * 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
ATE423314T1 (de) * 1998-06-24 2009-03-15 Illumina Inc Dekodierung von matrixartig-angeordneten sensoren durch mikropartikel
US6489096B1 (en) 1998-10-15 2002-12-03 Princeton University Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays
US6607888B2 (en) 1998-10-20 2003-08-19 Wisconsin Alumni Research Foundation Method for analyzing nucleic acid reactions
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US7612020B2 (en) * 1998-12-28 2009-11-03 Illumina, Inc. Composite arrays utilizing microspheres with a hybridization chamber
US6671044B2 (en) 1999-01-25 2003-12-30 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells in broad flat flow
US6707551B2 (en) * 2000-01-24 2004-03-16 Amnis Corporation Multipass cavity for illumination and excitation of moving objects
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US7450229B2 (en) * 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US6608682B2 (en) 1999-01-25 2003-08-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US7057732B2 (en) * 1999-01-25 2006-06-06 Amnis Corporation Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US6975400B2 (en) * 1999-01-25 2005-12-13 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
EP1157131A2 (de) * 1999-02-22 2001-11-28 Lynx Therapeutics, Inc. Polymorfische dns fragmente und dessen benutzung
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CA2366459A1 (en) * 1999-03-26 2000-10-05 Affymetrix, Inc. Universal arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6465183B2 (en) * 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
WO2001016352A1 (en) * 1999-08-27 2001-03-08 Phylos, Inc. Methods for encoding and sorting in vitro translated proteins
US20010031467A1 (en) * 1999-12-10 2001-10-18 Johannes Dapprich Method for selectively isolating a nucleic acid
JP2003517842A (ja) * 1999-12-21 2003-06-03 オーソ−クリニカル・ダイアグノスティックス・インコーポレイテッド 核酸の検出方法
EP1244782A2 (de) * 1999-12-23 2002-10-02 Axaron Bioscience AG Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
US20020009728A1 (en) * 2000-01-18 2002-01-24 Quantum Dot Corporation Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6770441B2 (en) * 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
EP1257805B1 (de) * 2000-02-10 2015-10-14 Illumina, Inc. Element mit einem substrat mit mehreren immunoassay-stellen zur simultanen analyse mehrerer proben mit hilfe von mikrokugeln, das element enthaltende vorrichtung, und herstellungsverfahren für das element
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
US7157564B1 (en) 2000-04-06 2007-01-02 Affymetrix, Inc. Tag nucleic acids and probe arrays
CA2405412A1 (en) * 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
US6778263B2 (en) * 2000-08-25 2004-08-17 Amnis Corporation Methods of calibrating an imaging system using calibration beads
US6934408B2 (en) * 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
US6583865B2 (en) 2000-08-25 2003-06-24 Amnis Corporation Alternative detector configuration and mode of operation of a time delay integration particle analyzer
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
WO2002025288A2 (en) * 2000-09-22 2002-03-28 Clontech Laboratories Inc. Highly sensitive proteomic analysis methods and kits and systems for practicing the same
WO2002031182A2 (en) * 2000-10-12 2002-04-18 Amnis Corporation Method and apparatus for reading reporter labeled beads
US7009651B2 (en) 2000-10-12 2006-03-07 Amnis Corporation System and method for high numeric aperture imaging systems
AU2002230524A1 (en) 2000-11-16 2002-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US7226737B2 (en) 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
CA2435551C (en) * 2001-01-25 2011-10-25 Tm Bioscience Corporation Families of non-cross-hybridizing polynucleotides for use as tags and tag complements, manufacture and use thereof
US20040266021A9 (en) * 2001-01-26 2004-12-30 Andras Guttman Multicapillary fraction collection system and method
AU2002324422A1 (en) * 2001-02-21 2002-12-23 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
JP2004523243A (ja) * 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
WO2002081729A2 (en) 2001-04-06 2002-10-17 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
WO2002086090A2 (en) * 2001-04-23 2002-10-31 Elitra Pharmaceuticals, Inc. Identification of essential genes of aspegillus fumigatus and methods of use
CA2445044C (en) * 2001-04-25 2011-02-15 Amnis Corporation Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging
US20070184436A1 (en) * 2001-06-07 2007-08-09 Joel Myerson Generic capture probe arrays
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US20060234231A1 (en) * 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7190832B2 (en) 2001-07-17 2007-03-13 Amnis Corporation Computational methods for the segmentation of images of objects from background in a flow imaging instrument
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
US20030180764A1 (en) * 2002-01-09 2003-09-25 Lynx Therapeutics, Inc. Genes affected by cholesterol treatment and during adipogenesis
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
CA2478985A1 (en) * 2002-03-13 2003-09-25 Syngenta Participations Ag Nucleic acid detection method
EP1487978B1 (de) 2002-03-15 2008-11-19 Nuevolution A/S Eine verbesserte methode zur synthese von matritzenabhängigen molekülen
EP1499706A4 (de) 2002-04-01 2010-11-03 Fluidigm Corp Mikrofluidische partikelanalysesysteme
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
AU2003249681A1 (en) 2002-05-31 2003-12-19 Diversa Corporation Multiplexed systems for nucleic acid sequencing
WO2003106672A2 (en) * 2002-06-12 2003-12-24 Riken METHOD FOR UTILIZING THE 5'END OF mRNA FOR CLONING AND ANALYSIS
AU2003277853A1 (en) * 2002-06-24 2004-01-06 Fluidigm Corporation Recirculating fluidic network and methods for using the same
DE10231684A1 (de) * 2002-07-12 2004-02-12 Micronas Holding Gmbh Verfahren zur Bestimmung der Zahl von Rezeptoren auf einem Träger sowie Biosensor herstellbar nach diesem Verfahren
AU2003247266A1 (en) 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
CA2500283A1 (en) 2002-09-25 2004-04-08 California Institute Of Technology Microfluidic large scale integration
WO2004028682A2 (en) * 2002-09-27 2004-04-08 Carlsberg A/S Spatially encoded polymer matrix
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
ES2368215T3 (es) 2002-10-30 2011-11-15 Nuevolution A/S Codificación enzimática.
US20040086892A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
EP1756277B1 (de) 2002-12-19 2009-12-02 Nuevolution A/S Durch quasizufallsstrukturen und funktionen geführte synthesemethode
CA2513985C (en) * 2003-01-21 2012-05-29 Illumina Inc. Chemical reaction monitor
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2005003375A2 (en) * 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
WO2004074429A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S Method for producing second-generation library
US20060269920A1 (en) * 2003-02-21 2006-11-30 Nuevolution A/S Method for obtaining structural information about an encoded molecule
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US7195875B2 (en) * 2003-04-18 2007-03-27 Beckman Coulter, Inc. Oligonucleotide pairs for multiplexed binding assays
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US11118215B2 (en) 2003-09-18 2021-09-14 Nuevolution A/S Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
US20050094807A1 (en) * 2003-11-04 2005-05-05 John Silzel Accuracy array assay system and method
WO2005047474A2 (en) * 2003-11-10 2005-05-26 Geneohm Sciences, Inc. Nucleic acid detection method having increased sensitivity
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
DE602005018166D1 (de) * 2004-02-12 2010-01-21 Population Genetics Technologi Genetische analyse mittels sequenzspezifischem sortieren
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
WO2005090945A1 (en) 2004-03-16 2005-09-29 Amnis Corporation Method for imaging and differential analysis of cells
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells
WO2005098430A2 (en) 2004-03-16 2005-10-20 Amnis Corporation Image based quantitation of molecular translocation
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
US20060019304A1 (en) * 2004-07-26 2006-01-26 Paul Hardenbol Simultaneous analysis of multiple genomes
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
WO2006020617A1 (en) * 2004-08-09 2006-02-23 Generation Biotech, Llc Method for nucleic acid isolation and amplification
US7170050B2 (en) * 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP1790202A4 (de) * 2004-09-17 2013-02-20 Pacific Biosciences California Vorrichtung und verfahren zur analyse von molekülen
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US9002652B1 (en) 2005-01-27 2015-04-07 Institute For Systems Biology Methods for identifying and using organ-specific proteins in blood
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2006084132A2 (en) * 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US20060204971A1 (en) * 2005-03-11 2006-09-14 Varde Shobha A Oligonucleotides for multiplexed binding assays
US20060211030A1 (en) * 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
US20060246576A1 (en) * 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2007067999A2 (en) 2005-12-09 2007-06-14 Amnis Corporation Extended depth of field imaging for high speed object analysis
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
US7537897B2 (en) * 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
EP3002339B1 (de) * 2006-02-02 2019-05-08 The Board of Trustees of The Leland Stanford Junior University Nicht-invasives fötales genetisches screening mittels digitaler analyse
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) * 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
WO2007098427A2 (en) * 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
US7815868B1 (en) * 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
CN101495654A (zh) * 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (de) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
US20080003667A1 (en) * 2006-05-19 2008-01-03 Affymetrix, Inc. Consumable elements for use with fluid processing and detection systems
US20080070792A1 (en) 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
CA2660286A1 (en) 2006-08-09 2008-02-21 Homestead Clinical Corporation Organ-specific proteins and methods of their use
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
EP2064488B1 (de) * 2006-09-01 2021-10-06 Pacific Biosciences of California, Inc. Gerät und verfahren zum analysieren von materialien
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
ES2490601T3 (es) * 2006-11-15 2014-09-04 Biospherex Llc Secuenciación multi-etiqueta y análisis ecogenómico
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
US7635566B2 (en) * 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US8222040B2 (en) * 2007-08-28 2012-07-17 Lightspeed Genomics, Inc. Nucleic acid sequencing by selective excitation of microparticles
US8759077B2 (en) * 2007-08-28 2014-06-24 Lightspeed Genomics, Inc. Apparatus for selective excitation of microparticles
WO2009040682A2 (en) * 2007-09-26 2009-04-02 Population Genetics Technologies Ltd. Methods and compositions for reducing the complexity of a nucleic acid sample
EP2212434A1 (de) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase-ligation-sequenzierung
EP3425060B1 (de) 2008-03-28 2021-10-27 Pacific Biosciences of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
US20100062946A1 (en) * 2008-09-08 2010-03-11 Lis John T Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution
EP4325209A2 (de) 2008-09-16 2024-02-21 Pacific Biosciences Of California, Inc. Integrierte optische vorrichtung
EA028985B1 (ru) 2009-02-13 2018-01-31 Икс-Чем, Инк. Способ идентификации соединений в составе библиотек, кодируемых днк
EP2411148B1 (de) 2009-03-23 2018-02-21 Raindance Technologies, Inc. Manipulation von mikrofluidiktröpfchen
US9085798B2 (en) * 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2425240A4 (de) 2009-04-30 2012-12-12 Good Start Genetics Inc Verfahren und zusammensetzung zur bewertung genetischer marker
US8451524B2 (en) 2009-09-29 2013-05-28 Amnis Corporation Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile
EP2486409A1 (de) 2009-10-09 2012-08-15 Universite De Strasbourg Markiertes nanomaterial auf siliziumbasis mit verbesserten eigenschaften und seine verwendung
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP3392349A1 (de) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digitale analyse von analyten
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US8994946B2 (en) 2010-02-19 2015-03-31 Pacific Biosciences Of California, Inc. Integrated analytical system and method
EP2537019B1 (de) 2010-02-19 2016-09-07 Pacific Biosciences Of California, Inc. Vorrichtung zur messung von analytischen reaktionen
US8502867B2 (en) 2010-03-19 2013-08-06 Lightspeed Genomics, Inc. Synthetic aperture optics imaging method using minimum selective excitation patterns
US9465228B2 (en) 2010-03-19 2016-10-11 Optical Biosystems, Inc. Illumination apparatus optimized for synthetic aperture optics imaging using minimum selective excitation patterns
DK2558577T3 (en) 2010-04-16 2019-04-01 Nuevolution As Bi-functional complexes and methods for the preparation and use of such complexes
US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
US8817115B1 (en) 2010-05-05 2014-08-26 Amnis Corporation Spatial alignment of image data from a multichannel detector using a reference image
EP2619327B1 (de) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Erhöhung der verlässlichkeit von allel-aufrufen mit molekülzählung
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP3412778A1 (de) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Verfahren zur bildung gemischter tröpfchen
EP3736281A1 (de) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2714970B1 (de) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzymquantifizierung
AU2012272161B2 (en) 2011-06-21 2015-12-24 Illumina Cambridge Limited Methods and systems for data analysis
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
MX360438B (es) 2011-09-07 2018-11-01 X Chem Inc Metodos para etiquetar bibliotecas codificadas por adn.
US9453258B2 (en) 2011-09-23 2016-09-27 Illumina, Inc. Methods and compositions for nucleic acid sequencing
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
WO2013096460A1 (en) 2011-12-20 2013-06-27 The Regents Of The University Of Michigan Pseudogenes and uses thereof
US10150993B2 (en) 2011-12-22 2018-12-11 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
US9506113B2 (en) 2011-12-28 2016-11-29 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US9803231B2 (en) 2011-12-29 2017-10-31 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
ES2665252T3 (es) 2011-12-30 2018-04-25 Abbott Molecular Inc. Purificación de ácido nucleico de microorganismos a partir de muestras del hospedador
CA2860594A1 (en) 2012-01-09 2013-07-18 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
CN108611398A (zh) 2012-01-13 2018-10-02 Data生物有限公司 通过新一代测序进行基因分型
RS61631B1 (sr) 2012-02-17 2021-04-29 Hutchinson Fred Cancer Res Kompozicije i postupci za preciznu identifikaciju mutacija
US9506117B2 (en) 2012-02-21 2016-11-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
WO2013126741A1 (en) 2012-02-24 2013-08-29 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP3287531B1 (de) 2012-02-28 2019-06-19 Agilent Technologies, Inc. Verfahren zur befestigung einer zählersequenz an einer nukleinsäureprobe
CA2866254A1 (en) 2012-03-06 2013-09-12 Oslo Universitetssykehus Hf Gene signatures associated with efficacy of postmastectomy radiotherapy in breast cancer
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
EP3789502A1 (de) 2012-05-02 2021-03-10 Ibis Biosciences, Inc. Dna-sequenzierung
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
WO2013166303A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
EP2844775B1 (de) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna-sequenzierung
US9372308B1 (en) 2012-06-17 2016-06-21 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices and methods for production
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
EA201992285A1 (ru) 2012-07-13 2020-05-31 Икс-Чем, Инк. Днк-кодированные библиотеки, содержащие связи кодирующих олигонуклеотидов, не доступные для считывания полимеразами
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102028375B1 (ko) 2012-09-04 2019-10-04 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US9223084B2 (en) 2012-12-18 2015-12-29 Pacific Biosciences Of California, Inc. Illumination of optical analytical devices
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US9624540B2 (en) 2013-02-22 2017-04-18 Pacific Biosciences Of California, Inc. Integrated illumination of optical analytical devices
WO2014160233A1 (en) 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US8778609B1 (en) 2013-03-14 2014-07-15 Good Start Genetics, Inc. Methods for analyzing nucleic acids
US20140287946A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
CN105378107A (zh) 2013-03-14 2016-03-02 雅培分子公司 多重甲基化-特异性扩增系统和方法
US9890425B2 (en) 2013-03-15 2018-02-13 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
EP3533884A1 (de) 2013-03-15 2019-09-04 Ibis Biosciences, Inc. Dna-sequenzen zur beurteilung der kontamination in der dna-sequenzierung
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
EP2986762B1 (de) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digitale analyse von analyten
EP3005200A2 (de) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Verfahren und systeme zur speicherung von sequenzlesedaten
EP3626866B1 (de) 2013-08-19 2021-03-24 Abbott Molecular Inc. Sequenzierungsbibliotheken der nächsten generation
EP3036248B1 (de) 2013-08-19 2020-05-27 Abbott Molecular Inc. Nukleotidanaloga
DK3039158T3 (en) 2013-08-28 2019-03-04 Becton Dickinson Co MASSIVE PARALLEL SINGLE CELL CELL ANALYSIS
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
EP3875601A1 (de) 2013-10-17 2021-09-08 Illumina, Inc. Verfahren und zusammensetzungen zur herstellung von nukleinsäurebibliotheken
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015073707A1 (en) 2013-11-15 2015-05-21 The Board Of Trustees Of The Leland Stanford Junior University Methods of treating heart failure with agonists of hypocretin receptor 2
EP3080268A1 (de) 2013-12-09 2016-10-19 Illumina, Inc. Verfahren und zusammensetzungen zur gezielten nukleinsäuresequenzierung
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
EP3083700B1 (de) 2013-12-17 2023-10-11 The Brigham and Women's Hospital, Inc. Bestimmung eines antikörpers gegen ein pathogen
CN106062214B (zh) 2013-12-28 2020-06-09 夸登特健康公司 用于检测遗传变异的方法和系统
EP3090063B1 (de) 2013-12-31 2019-11-06 Bio-Rad Laboratories, Inc. Verfahren zur detektion von latentem retrovirus
US9896686B2 (en) 2014-01-09 2018-02-20 AgBiome, Inc. High throughput discovery of new genes from complex mixtures of environmental microbes
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
US10760109B2 (en) 2014-06-06 2020-09-01 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
JP2017521654A (ja) 2014-06-27 2017-08-03 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ ヒトペギウイルス２（ＨＰｇＶ−２）を検出するための組成物および方法
US10072306B2 (en) 2014-07-24 2018-09-11 Abbott Molecular Inc. Compositions and methods for the detection and analysis of mycobacterium tuberculosis
US10093967B2 (en) 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
CA2959518A1 (en) 2014-08-27 2016-03-03 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
CN107002293B (zh) 2014-09-17 2020-12-08 艾比斯生物科学公司 使用脉冲读数光学的通过合成测序
EP3224595A4 (de) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Prozesssteuerung für erhöhte robustheit von genetischen assays
WO2016061514A1 (en) 2014-10-17 2016-04-21 Good Start Genetics, Inc. Pre-implantation genetic screening and aneuploidy detection
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
EP3766988B1 (de) 2015-02-19 2024-02-14 Becton, Dickinson and Company Hochdurchsatzeinzelzellenanalyse mit kombination von proteomischen und genomischen informationen
EP3259602B9 (de) 2015-02-20 2021-05-19 Takara Bio USA, Inc. Verfahren zur schnellen präzisen abgabe, visualisierung und analyse von einzelzellen
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016149397A1 (en) 2015-03-16 2016-09-22 Pacific Biosciences Of California, Inc. Integrated devices and systems for free-space optical coupling
EP3277843A2 (de) 2015-03-30 2018-02-07 Cellular Research, Inc. Verfahren und zusammensetzungen für kombinatorische barcodierung
EP3286326A1 (de) 2015-04-23 2018-02-28 Cellular Research, Inc. Verfahren und zusammensetzungen zur gesamttranskriptomamplifikation
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
EP3307908B1 (de) 2015-06-09 2019-09-11 Life Technologies Corporation Verfahren zur molekularen markierung
WO2016201387A1 (en) 2015-06-12 2016-12-15 Pacific Biosciences Of California, Inc. Integrated target waveguide devices and systems for optical coupling
WO2017011538A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
CN108184327A (zh) 2015-07-14 2018-06-19 雅培分子公司 用于鉴定耐药性结核的组合物和方法
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
WO2017120531A1 (en) 2016-01-08 2017-07-13 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
EA037304B1 (ru) 2016-01-27 2021-03-09 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк
JP6902052B2 (ja) 2016-02-08 2021-07-14 アールジーン・インコーポレイテッドＲｇｅｎｅ， Ｉｎｃ． 複数のリガーゼ組成物、システム、および方法
WO2017160788A2 (en) 2016-03-14 2017-09-21 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017168332A1 (en) 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex target capture
US10822643B2 (en) 2016-05-02 2020-11-03 Cellular Research, Inc. Accurate molecular barcoding
US11118233B2 (en) 2016-05-18 2021-09-14 The University Of Chicago BTK mutation and ibrutinib resistance
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
WO2017218908A2 (en) 2016-06-16 2017-12-21 The Johns Hopkins University Methods and system for epigenetic analysis
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices
ES2961743T3 (es) 2016-09-26 2024-03-13 Becton Dickinson Co Medición de la expresión de proteínas utilizando reactivos con secuencias de oligonucleótidos con código de barras
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
CN109906274B (zh) 2016-11-08 2023-08-25 贝克顿迪金森公司 用于细胞标记分类的方法
JP7232180B2 (ja) 2016-11-08 2023-03-02 ベクトン・ディキンソン・アンド・カンパニー 発現プロファイル分類の方法
US11268137B2 (en) 2016-12-09 2022-03-08 Boreal Genomics, Inc. Linked ligation
EP3555290B1 (de) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Markierte dna mit erhaltener tröpfchenmarkierungskontinuität
US10722880B2 (en) 2017-01-13 2020-07-28 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CN110446787A (zh) 2017-03-24 2019-11-12 生物辐射实验室股份有限公司 通用发夹引物
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
WO2018227091A1 (en) 2017-06-08 2018-12-13 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
WO2018236918A1 (en) 2017-06-20 2018-12-27 Bio-Rad Laboratories, Inc. MDA USING A BALL OLIGONUCLEOTIDE
KR20200075814A (ko) 2017-08-15 2020-06-26 옴니옴 인코포레이티드 화학적 및 생물학적 분석물의 검출에 유용한 스캐닝 장치 및 방법
WO2019071164A1 (en) 2017-10-06 2019-04-11 The University Of Chicago SCREENING OF LYMPHOCYTES T FOR ANTIGENS SPECIFIC TO CANCER
CN111295443B (zh) 2017-11-02 2024-04-16 生物辐射实验室股份有限公司 基于转座酶的基因组分析
EP3714043A4 (de) 2017-11-22 2021-08-11 The Regents of The University of Michigan Zusammensetzungen und verfahren zur behandlung von krebs
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
EP3728636A1 (de) 2017-12-19 2020-10-28 Becton, Dickinson and Company Mit oligonukleotiden assoziierte teilchen
WO2019136058A1 (en) 2018-01-02 2019-07-11 The Regents Of The University Of Michigan Multi-droplet capture
US11366303B2 (en) 2018-01-30 2022-06-21 Rebus Biosystems, Inc. Method for detecting particles using structured illumination
US20190241944A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
WO2019195379A1 (en) 2018-04-04 2019-10-10 Lifeedit, Inc. Methods and compositions to identify novel crispr systems
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP7407128B2 (ja) 2018-05-03 2023-12-28 ベクトン・ディキンソン・アンド・カンパニー ハイスループットマルチオミクスサンプル解析
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
EP3788377A1 (de) 2018-05-04 2021-03-10 Abbott Laboratories Hbv-diagnostische, prognostische und therapeutische verfahren und produkte
CN113166807A (zh) 2018-08-20 2021-07-23 生物辐射实验室股份有限公司 通过分区中条码珠共定位生成核苷酸序列
EP3861134A1 (de) 2018-10-01 2021-08-11 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020117653A1 (en) 2018-12-04 2020-06-11 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US11041199B2 (en) 2018-12-20 2021-06-22 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
WO2020141464A1 (en) 2019-01-03 2020-07-09 Boreal Genomics, Inc. Linked target capture
WO2020142768A1 (en) 2019-01-04 2020-07-09 Northwestern University Storing temporal data into dna
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (de) 2019-01-23 2023-04-05 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
EP3927467A4 (de) 2019-02-20 2022-12-14 Pacific Biosciences of California, Inc. Scanner und verfahren zum nachweis von chemischen und biologischen analyten
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
EP4004231A1 (de) 2019-07-22 2022-06-01 Becton, Dickinson and Company Test für einzelzell-chromatin-immunpräzipitationssequenzierung
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
CN114728996B (zh) 2019-09-10 2022-11-29 加利福尼亚太平洋生物科学股份有限公司 核苷酸的可逆修饰
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
WO2021092431A1 (en) 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
EP4100161A1 (de) 2020-02-04 2022-12-14 Pacific Biosciences of California, Inc. Durchflusszellen und verfahren zu deren herstellung und verwendung
JP2023513314A (ja) 2020-02-13 2023-03-30 ザイマージェン インコーポレイテッド メタゲノムライブラリーおよび天然物発見プラットフォーム
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
EP4150118A1 (de) 2020-05-14 2023-03-22 Becton Dickinson and Company Primer zur profilierung von immunrepertoire
KR20230019872A (ko) 2020-05-27 2023-02-09 지네틱 테크놀로지스 리미티드 코로나바이러스 감염에 대한 중증 반응의 발생 위험을 평가하는 방법
EP4231174A3 (de) 2020-06-11 2023-11-01 Nautilus Subsidiary, Inc. Verfahren und syteme zur rechnerischen decodierung biologischer, chemischer und physikalischer einheiten
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4244382A1 (de) 2020-11-11 2023-09-20 Nautilus Subsidiary, Inc. Affinitätsreagenzien mit verbesserten bindungs- und nachweiseigenschaften
CN116635533A (zh) 2020-11-20 2023-08-22 贝克顿迪金森公司 高表达的蛋白和低表达的蛋白的谱分析
CA3203535A1 (en) 2021-01-21 2022-07-28 Gregory KAPP Systems and methods for biomolecule preparation
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
AU2022232933A1 (en) 2021-03-11 2023-09-07 Nautilus Subsidiary, Inc. Systems and methods for biomolecule retention
US20230070896A1 (en) 2021-09-09 2023-03-09 Nautilus Biotechnology, Inc. Characterization and localization of protein modifications
WO2023049073A1 (en) 2021-09-22 2023-03-30 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
WO2023135485A1 (en) 2022-01-13 2023-07-20 Oslo Universitetssykehus Hf Prostate cancer markers and uses thereof
WO2023152568A2 (en) 2022-02-10 2023-08-17 Oslo Universitetssykehus Hf Compositions and methods for characterizing lung cancer
US20230314324A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5149625A (en) * 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
EP0392546A3 (de) * 1989-04-14 1991-09-11 Ro Institut Za Molekularnu Genetiku I Geneticko Inzenjerstvo Verfahren zur Bestimmung von vollständigen oder partiellen Bestandteilen von sehr kurzen Sequenzen in Proben von Partikeln mikroskopischer Grösse mittels Hybridisierung mit Oligonukleotid-Sonden
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5650489A (en) * 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
EP0544809B1 (de) * 1990-08-24 1998-12-16 Ixsys, Inc. Verfahren zur herstellung von oligonukleotiden mit regellosen codonen
EP0562047A4 (en) * 1990-12-06 1995-11-02 Affymax Tech Nv Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
US5312157A (en) * 1991-02-20 1994-05-17 Logan Jr Emanuel Lift seat
JP2001524926A (ja) * 1991-09-18 2001-12-04 アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ オリゴマーの雑多ライブラリーの集合体を合成する方法
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
WO1993013220A1 (en) * 1991-12-24 1993-07-08 Tepnel Medical Limited Manipulating nucleic acid sequences
DE69333650T2 (de) * 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
EP0638089B2 (de) * 1992-04-15 2008-03-05 The Johns Hopkins University Synthese von verschiedenen und nützlichen oligonukleotidsammlungen
US5541061A (en) * 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
ATE244769T1 (de) * 1992-10-01 2003-07-15 Univ Columbia Komplexe kombinatorische chemische banken, die mit markierungen versehen sind
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
US5593826A (en) * 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
US5473060A (en) * 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5539083A (en) * 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage

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EP0952216B1 (de) 2005-08-17
WO1996012039A1 (en) 1996-04-25
US5695934A (en) 1997-12-09
EP0952216A2 (de) 1999-10-27
EP0786014B1 (de) 1999-12-15
US5863722A (en) 1999-01-26
US6140489A (en) 2000-10-31
EP0952216A3 (de) 2000-01-19
DE69513997T2 (de) 2000-07-27
DE69513997D1 (de) 2000-01-20
DE69534389T2 (de) 2006-06-29
EP0786014A1 (de) 1997-07-30
AU3946195A (en) 1996-05-06
EP0952216B8 (de) 2005-10-19

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