MX360438B - Metodos para etiquetar bibliotecas codificadas por adn. - Google Patents

Metodos para etiquetar bibliotecas codificadas por adn.

Info

Publication number
MX360438B
MX360438B MX2014002854A MX2014002854A MX360438B MX 360438 B MX360438 B MX 360438B MX 2014002854 A MX2014002854 A MX 2014002854A MX 2014002854 A MX2014002854 A MX 2014002854A MX 360438 B MX360438 B MX 360438B
Authority
MX
Mexico
Prior art keywords
methods
encoded libraries
libraries
tagging dna
tagging
Prior art date
Application number
MX2014002854A
Other languages
English (en)
Other versions
MX2014002854A (es
Inventor
D Keefe Anthony
w wagner Richard
Litovchick Alexander
Clark Matthew
W Cuozzo John
Zhang Ying
A Centrella Paolo
D Hupp Christopher
Original Assignee
X Chem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47832597&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX360438(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by X Chem Inc filed Critical X Chem Inc
Publication of MX2014002854A publication Critical patent/MX2014002854A/es
Publication of MX360438B publication Critical patent/MX360438B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/16—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support involving encoding steps
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Structural Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

La presente invención se refiere a un método de etiquetar una primera biblioteca que comprende una entidad química codificada por oligonucleótido, dicho método comprendiendo: (i) proveer una pieza de cabeza que consiste en un oligonucleótido de una sola cadena que tiene un primer grupo funcional y un segundo grupo funcional, donde dicha pieza de cabeza comprende al menos un nucleótido 2´-sustituido terminal que comprende dicho segundo grupo funcional, donde dicha pieza de cabeza comprende un nucleótido 2´-sustituido en el término 5´y/o el término 3´; (ii) ligar dicho primer grupo funcional de dicha pieza de cabeza a un primer componente de dicha entidad química, donde dicha pieza de cabeza está directamente a dicho primer componente o dicha pieza de cabeza está conectada indirectamente a dicho primer componente mediante un enlazador bifuncional; y (iii) ligar dicho segundo grupo funcional de dicha pieza de cabeza a una etiqueta de un primer bloque de construcción que consiste en un oligonucleótido de una sola cadena para formar un complejo, donde la etiqueta de primer bloque de construcción comprende un nucleótido 2´- sustituido terminal en el término 5´y el término 3´y la ligación comprende ligación enzimática; donde los pasos (ii) y (iii) puede ser llevados a cabo en cualquier orden y donde dicha etiqueta de primer bloque de construcción codifica la reacción de ligadura de dicho paso (ii), con ello proveyendo una biblioteca etiquetada.
MX2014002854A 2011-09-07 2012-09-07 Metodos para etiquetar bibliotecas codificadas por adn. MX360438B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161531820P 2011-09-07 2011-09-07
US201161536929P 2011-09-20 2011-09-20
PCT/US2012/054228 WO2013036810A1 (en) 2011-09-07 2012-09-07 Methods for tagging dna-encoded libraries

Publications (2)

Publication Number Publication Date
MX2014002854A MX2014002854A (es) 2016-06-24
MX360438B true MX360438B (es) 2018-11-01

Family

ID=47832597

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014002854A MX360438B (es) 2011-09-07 2012-09-07 Metodos para etiquetar bibliotecas codificadas por adn.
MX2018013367A MX2018013367A (es) 2011-09-07 2014-03-07 Metodos para etiquetar bibliotecas codificadas por adn.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2018013367A MX2018013367A (es) 2011-09-07 2014-03-07 Metodos para etiquetar bibliotecas codificadas por adn.

Country Status (17)

Country Link
US (2) US10865409B2 (es)
EP (3) EP3828271A1 (es)
JP (3) JP6674738B2 (es)
KR (2) KR102242879B1 (es)
CN (1) CN103998658B (es)
AP (1) AP2014007483A0 (es)
AU (3) AU2012304387B2 (es)
BR (1) BR112014005205A2 (es)
CA (2) CA2848023C (es)
DK (2) DK3351631T3 (es)
EA (1) EA032438B1 (es)
ES (2) ES2852073T3 (es)
IL (2) IL231191B (es)
IN (1) IN2014CN02574A (es)
MX (2) MX360438B (es)
SG (2) SG11201400374TA (es)
WO (1) WO2013036810A1 (es)

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