DE69328986D1 - Konfokales Rastermikroskop - Google Patents

Konfokales Rastermikroskop

Info

Publication number
DE69328986D1
DE69328986D1 DE69328986T DE69328986T DE69328986D1 DE 69328986 D1 DE69328986 D1 DE 69328986D1 DE 69328986 T DE69328986 T DE 69328986T DE 69328986 T DE69328986 T DE 69328986T DE 69328986 D1 DE69328986 D1 DE 69328986D1
Authority
DE
Germany
Prior art keywords
scanning microscope
confocal scanning
confocal
microscope
scanning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69328986T
Other languages
English (en)
Other versions
DE69328986T2 (de
Inventor
Roger Y Tsien
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Application granted granted Critical
Publication of DE69328986D1 publication Critical patent/DE69328986D1/de
Publication of DE69328986T2 publication Critical patent/DE69328986T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N1/00—Scanning, transmission or reproduction of documents or the like, e.g. facsimile transmission; Details thereof
    • H04N1/04—Scanning arrangements, i.e. arrangements for the displacement of active reading or reproducing elements relative to the original or reproducing medium, or vice versa
    • H04N1/047—Detection, control or error compensation of scanning velocity or position
    • H04N1/053—Detection, control or error compensation of scanning velocity or position in main scanning direction, e.g. synchronisation of line start or picture elements in a line
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/024—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof deleted
    • H04N2201/02406—Arrangements for positioning elements within a head
    • H04N2201/02439—Positioning method
    • H04N2201/02443—Positioning method using adhesive
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04701—Detection of scanning velocity or position
    • H04N2201/0471—Detection of scanning velocity or position using dedicated detectors
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04701—Detection of scanning velocity or position
    • H04N2201/04734—Detecting at frequent intervals, e.g. once per line for sub-scan control
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04701—Detection of scanning velocity or position
    • H04N2201/04744—Detection of scanning velocity or position by detecting the scanned beam or a reference beam
    • H04N2201/04746—Detection of scanning velocity or position by detecting the scanned beam or a reference beam after modulation by a grating, mask or the like
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04753—Control or error compensation of scanning position or velocity
    • H04N2201/04758—Control or error compensation of scanning position or velocity by controlling the position of the scanned image area
    • H04N2201/04767—Control or error compensation of scanning position or velocity by controlling the position of the scanned image area by controlling the timing of the signals, e.g. by controlling the frequency o phase of the pixel clock
    • H04N2201/04768—Controlling the frequency of the signals
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04753—Control or error compensation of scanning position or velocity
    • H04N2201/04794—Varying the control or compensation during the scan, e.g. using continuous feedback or from line to line
    • H04N2201/04798—Varying the main-scan control during the main-scan, e.g. facet tracking

Landscapes

  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Mechanical Optical Scanning Systems (AREA)
DE69328986T 1992-04-01 1993-03-26 Konfokales Rastermikroskop Expired - Fee Related DE69328986T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/862,633 US5296703A (en) 1992-04-01 1992-04-01 Scanning confocal microscope using fluorescence detection

Publications (2)

Publication Number Publication Date
DE69328986D1 true DE69328986D1 (de) 2000-08-17
DE69328986T2 DE69328986T2 (de) 2000-12-28

Family

ID=25338909

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69328986T Expired - Fee Related DE69328986T2 (de) 1992-04-01 1993-03-26 Konfokales Rastermikroskop

Country Status (4)

Country Link
US (1) US5296703A (de)
EP (1) EP0564178B1 (de)
JP (1) JPH07270307A (de)
DE (1) DE69328986T2 (de)

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US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US5880880A (en) * 1995-01-13 1999-03-09 The General Hospital Corp. Three-dimensional scanning confocal laser microscope
US5785651A (en) * 1995-06-07 1998-07-28 Keravision, Inc. Distance measuring confocal microscope
US6175754B1 (en) 1995-06-07 2001-01-16 Keravision, Inc. Method and apparatus for measuring corneal incisions
US5585639A (en) * 1995-07-27 1996-12-17 Hewlett-Packard Company Optical scanning apparatus
US5874726A (en) * 1995-10-10 1999-02-23 Iowa State University Research Foundation Probe-type near-field confocal having feedback for adjusting probe distance
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WO1997043611A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Systems and methods for detection of labeled materials
JP3716502B2 (ja) * 1996-07-24 2005-11-16 東ソー株式会社 蛍光検出装置
US5763870A (en) * 1996-12-13 1998-06-09 Hewlett-Packard Company Method and system for operating a laser device employing an integral power-regulation sensor
US5887009A (en) * 1997-05-22 1999-03-23 Optical Biopsy Technologies, Inc. Confocal optical scanning system employing a fiber laser
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CA2318892A1 (en) 1998-01-27 1999-07-29 Wisconsin Alumni Research Foundation Signal enhancement for fluorescence microscopy
DE59915024D1 (de) * 1998-02-20 2009-06-25 Leica Microsystems Anordnung zum kalibrieren eines laserscanmikroskops
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US5880006A (en) 1998-05-22 1999-03-09 Vlsi Technology, Inc. Method for fabrication of a semiconductor device
US6838680B2 (en) * 1999-05-12 2005-01-04 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
JP3815969B2 (ja) * 1999-05-12 2006-08-30 アクララ バイオサイエンシーズ， インコーポレイテッド 微量流体デバイスにおける多重方式蛍光検出
US6218803B1 (en) 1999-06-04 2001-04-17 Genetic Microsystems, Inc. Position sensing with variable capacitance transducers
US6423960B1 (en) * 1999-12-31 2002-07-23 Leica Microsystems Heidelberg Gmbh Method and system for processing scan-data from a confocal microscope
US6433929B1 (en) * 2000-06-12 2002-08-13 Olympus Optical Co., Ltd. Scanning optical microscope and method of acquiring image
US20040224421A1 (en) * 2000-06-15 2004-11-11 Deweerd Herman Bi-directional scanning method
US6687000B1 (en) 2000-06-26 2004-02-03 Wisconsin Alumni Research Foundation Photon-sorting spectroscopic microscope system
DE10065784C2 (de) * 2000-12-30 2003-12-04 Leica Microsystems Verfahren zum Auffinden von Kontaktstellen zwischen Zellen in einer stimulierbaren mikroskopischen Probe bei einer Kalziummigration und Scanmikroskop zur Durchführung des Verfahrens
US6859273B2 (en) * 2001-07-23 2005-02-22 University Of Rochester Method for operating a laser scanning confocal microscope system and a system thereof
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
DE10231776B4 (de) * 2002-07-13 2021-07-22 Leica Microsystems Cms Gmbh Verfahren zur Scanmikroskopie und Scanmikroskop
WO2005074417A2 (en) * 2003-09-03 2005-08-18 Salk Institute For Biological Studies Multiple antigen detection assays and reagents
DE10351414A1 (de) * 2003-10-30 2005-06-23 Carl Zeiss Jena Gmbh Laser-Scanning-Mikroskop mit einem non-descannten Detektions- und/oder Beobachtungsstrahlengang
US6940641B2 (en) * 2003-11-18 2005-09-06 Olympus Corporation Fluorescence observation apparatus
DE102005047884A1 (de) * 2005-10-06 2007-04-26 Leica Microsystems Cms Gmbh Scanmikroskop und Scanverfahren mit einem Scanmikroskop
JP5255218B2 (ja) * 2006-03-14 2013-08-07 株式会社リコー 画像処理方法
US7817275B2 (en) * 2007-03-07 2010-10-19 University Of Delaware Scanning optical microscope with long working distance objective
DE102008034137A1 (de) 2007-09-28 2009-04-02 Carl Zeiss Microlmaging Gmbh Mikroskop und Verfahren zum Betreiben eines Mikroskops
WO2010087994A2 (en) 2009-01-30 2010-08-05 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
JP5715068B2 (ja) 2009-01-30 2015-05-07 マイクロニクス， インコーポレイテッド 携帯型高利得蛍光検出システム
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
EP3667391A1 (de) * 2009-10-28 2020-06-17 Carl Zeiss Microscopy GmbH Mikroskopisches verfahren und mikroskop mit gesteigerter auflösung
WO2011094577A2 (en) 2010-01-29 2011-08-04 Micronics, Inc. Sample-to-answer microfluidic cartridge
EP2733153A1 (de) 2012-11-15 2014-05-21 INSERM (Institut National de la Santé et de la Recherche Médicale) Verfahren zur Herstellung von Immunkonjugaten und Verwendungen davon
CN104919035B (zh) 2012-12-21 2017-08-11 精密公司 便携式荧光检测系统和微测定盒
WO2014100743A2 (en) 2012-12-21 2014-06-26 Micronics, Inc. Low elasticity films for microfluidic use
WO2014100732A1 (en) 2012-12-21 2014-06-26 Micronics, Inc. Fluidic circuits and related manufacturing methods
DE102013001238B4 (de) * 2013-01-25 2020-06-10 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Mikroskopieverfahren
WO2014182847A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Device for preparation and analysis of nucleic acids
US10386377B2 (en) 2013-05-07 2019-08-20 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
JP6472788B2 (ja) 2013-05-07 2019-02-20 マイクロニクス， インコーポレイテッド 粘土鉱物およびアルカリ性溶液を使用して核酸含有試料を調製するための方法
US9485383B2 (en) 2013-12-04 2016-11-01 Canon Kabushiki Kaisha Image based correction of distortion from a scanner
WO2015163261A1 (ja) * 2014-04-24 2015-10-29 オリンパス株式会社 顕微鏡および顕微鏡観察方法
JP6594437B2 (ja) 2015-09-15 2019-10-23 オリンパス株式会社 顕微鏡および顕微鏡観察方法
ES2941968T3 (es) 2015-10-01 2023-05-29 The Whitehead Institute For Biomedical Res Marcaje de anticuerpos
WO2021183967A1 (en) * 2020-03-13 2021-09-16 University Of Southern California High throughput snapshot spectral encoding device for fluorescence spectral microscopy

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Also Published As

Publication number Publication date
EP0564178A1 (de) 1993-10-06
DE69328986T2 (de) 2000-12-28
US5296703A (en) 1994-03-22
JPH07270307A (ja) 1995-10-20
EP0564178B1 (de) 2000-07-12

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee