DE69328986T2 - Konfokales Rastermikroskop - Google Patents

Konfokales Rastermikroskop

Info

Publication number
DE69328986T2
DE69328986T2 DE69328986T DE69328986T DE69328986T2 DE 69328986 T2 DE69328986 T2 DE 69328986T2 DE 69328986 T DE69328986 T DE 69328986T DE 69328986 T DE69328986 T DE 69328986T DE 69328986 T2 DE69328986 T2 DE 69328986T2
Authority
DE
Germany
Prior art keywords
scanning microscope
confocal scanning
confocal
microscope
scanning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69328986T
Other languages
English (en)
Other versions
DE69328986D1 (de
Inventor
Roger Y Tsien
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Publication of DE69328986D1 publication Critical patent/DE69328986D1/de
Application granted granted Critical
Publication of DE69328986T2 publication Critical patent/DE69328986T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N1/00—Scanning, transmission or reproduction of documents or the like, e.g. facsimile transmission; Details thereof
    • H04N1/04—Scanning arrangements, i.e. arrangements for the displacement of active reading or reproducing elements relative to the original or reproducing medium, or vice versa
    • H04N1/047—Detection, control or error compensation of scanning velocity or position
    • H04N1/053—Detection, control or error compensation of scanning velocity or position in main scanning direction, e.g. synchronisation of line start or picture elements in a line
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/024—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof deleted
    • H04N2201/02406—Arrangements for positioning elements within a head
    • H04N2201/02439—Positioning method
    • H04N2201/02443—Positioning method using adhesive
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04701—Detection of scanning velocity or position
    • H04N2201/0471—Detection of scanning velocity or position using dedicated detectors
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04701—Detection of scanning velocity or position
    • H04N2201/04734—Detecting at frequent intervals, e.g. once per line for sub-scan control
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04701—Detection of scanning velocity or position
    • H04N2201/04744—Detection of scanning velocity or position by detecting the scanned beam or a reference beam
    • H04N2201/04746—Detection of scanning velocity or position by detecting the scanned beam or a reference beam after modulation by a grating, mask or the like
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04753—Control or error compensation of scanning position or velocity
    • H04N2201/04758—Control or error compensation of scanning position or velocity by controlling the position of the scanned image area
    • H04N2201/04767—Control or error compensation of scanning position or velocity by controlling the position of the scanned image area by controlling the timing of the signals, e.g. by controlling the frequency o phase of the pixel clock
    • H04N2201/04768—Controlling the frequency of the signals
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N2201/00—Indexing scheme relating to scanning, transmission or reproduction of documents or the like, and to details thereof
    • H04N2201/04—Scanning arrangements
    • H04N2201/047—Detection, control or error compensation of scanning velocity or position
    • H04N2201/04753—Control or error compensation of scanning position or velocity
    • H04N2201/04794—Varying the control or compensation during the scan, e.g. using continuous feedback or from line to line
    • H04N2201/04798—Varying the main-scan control during the main-scan, e.g. facet tracking

Landscapes

  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Mechanical Optical Scanning Systems (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
DE69328986T 1992-04-01 1993-03-26 Konfokales Rastermikroskop Expired - Fee Related DE69328986T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/862,633 US5296703A (en) 1992-04-01 1992-04-01 Scanning confocal microscope using fluorescence detection

Publications (2)

Publication Number Publication Date
DE69328986D1 DE69328986D1 (de) 2000-08-17
DE69328986T2 true DE69328986T2 (de) 2000-12-28

Family

ID=25338909

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69328986T Expired - Fee Related DE69328986T2 (de) 1992-04-01 1993-03-26 Konfokales Rastermikroskop

Country Status (4)

Country Link
US (1) US5296703A (de)
EP (1) EP0564178B1 (de)
JP (1) JPH07270307A (de)
DE (1) DE69328986T2 (de)

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US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
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US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
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JP3716502B2 (ja) * 1996-07-24 2005-11-16 東ソー株式会社 蛍光検出装置
US5763870A (en) * 1996-12-13 1998-06-09 Hewlett-Packard Company Method and system for operating a laser device employing an integral power-regulation sensor
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US6838680B2 (en) * 1999-05-12 2005-01-04 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
EP1192447A2 (de) 1999-05-12 2002-04-03 Aclara BioSciences, Inc. Multiplexer nachweis von fluoreszenz in mikrofluidischen vorrichtungen
US6218803B1 (en) 1999-06-04 2001-04-17 Genetic Microsystems, Inc. Position sensing with variable capacitance transducers
US6423960B1 (en) * 1999-12-31 2002-07-23 Leica Microsystems Heidelberg Gmbh Method and system for processing scan-data from a confocal microscope
US6433929B1 (en) * 2000-06-12 2002-08-13 Olympus Optical Co., Ltd. Scanning optical microscope and method of acquiring image
US20040224421A1 (en) * 2000-06-15 2004-11-11 Deweerd Herman Bi-directional scanning method
US6687000B1 (en) 2000-06-26 2004-02-03 Wisconsin Alumni Research Foundation Photon-sorting spectroscopic microscope system
DE10065784C2 (de) * 2000-12-30 2003-12-04 Leica Microsystems Verfahren zum Auffinden von Kontaktstellen zwischen Zellen in einer stimulierbaren mikroskopischen Probe bei einer Kalziummigration und Scanmikroskop zur Durchführung des Verfahrens
US6859273B2 (en) * 2001-07-23 2005-02-22 University Of Rochester Method for operating a laser scanning confocal microscope system and a system thereof
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
DE10231776B4 (de) * 2002-07-13 2021-07-22 Leica Microsystems Cms Gmbh Verfahren zur Scanmikroskopie und Scanmikroskop
US20050095627A1 (en) * 2003-09-03 2005-05-05 The Salk Institute For Biological Studies Multiple antigen detection assays and reagents
DE10351414A1 (de) * 2003-10-30 2005-06-23 Carl Zeiss Jena Gmbh Laser-Scanning-Mikroskop mit einem non-descannten Detektions- und/oder Beobachtungsstrahlengang
US6940641B2 (en) * 2003-11-18 2005-09-06 Olympus Corporation Fluorescence observation apparatus
DE102005047884A1 (de) * 2005-10-06 2007-04-26 Leica Microsystems Cms Gmbh Scanmikroskop und Scanverfahren mit einem Scanmikroskop
JP5255218B2 (ja) * 2006-03-14 2013-08-07 株式会社リコー 画像処理方法
US7817275B2 (en) * 2007-03-07 2010-10-19 University Of Delaware Scanning optical microscope with long working distance objective
DE102008034137A1 (de) 2007-09-28 2009-04-02 Carl Zeiss Microlmaging Gmbh Mikroskop und Verfahren zum Betreiben eines Mikroskops
WO2010088514A1 (en) 2009-01-30 2010-08-05 Micronics, Inc. Portable high gain fluorescence detection system
WO2010087994A2 (en) 2009-01-30 2010-08-05 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
EP2317362B1 (de) * 2009-10-28 2020-01-15 Carl Zeiss Microscopy GmbH Mikroskopisches Verfahren und Mikroskop mit gesteigerter Auflösung
WO2011094577A2 (en) 2010-01-29 2011-08-04 Micronics, Inc. Sample-to-answer microfluidic cartridge
EP2733153A1 (de) 2012-11-15 2014-05-21 INSERM (Institut National de la Santé et de la Recherche Médicale) Verfahren zur Herstellung von Immunkonjugaten und Verwendungen davon
KR20150097764A (ko) 2012-12-21 2015-08-26 마이크로닉스 인코포레이티드. 휴대형 형광 검출 시스템 및 미량분석 카트리지
CN107824233B (zh) 2012-12-21 2020-06-30 珀金埃尔默健康科学有限公司 用于微流体用途的低弹性膜
WO2014100732A1 (en) 2012-12-21 2014-06-26 Micronics, Inc. Fluidic circuits and related manufacturing methods
DE102013001238B4 (de) * 2013-01-25 2020-06-10 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Mikroskopieverfahren
EP2994543B1 (de) 2013-05-07 2018-08-15 Micronics, Inc. Vorrichtung zur herstellung und analyse von nukleinsäuren
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
US9485383B2 (en) 2013-12-04 2016-11-01 Canon Kabushiki Kaisha Image based correction of distortion from a scanner
DE112015001154T5 (de) * 2014-04-24 2016-12-08 Olympus Corporation Mikroskop und Mikroskopie-Verfahren
WO2017046863A1 (ja) 2015-09-15 2017-03-23 オリンパス株式会社 顕微鏡および顕微鏡観察方法
EP4118417A4 (de) * 2020-03-13 2024-05-01 Univ Southern California Vorrichtung zur spektralen kodierung von speicherauszügen mit hohem durchsatz für fluoreszenzspektralmikroskopie

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Also Published As

Publication number Publication date
EP0564178B1 (de) 2000-07-12
US5296703A (en) 1994-03-22
DE69328986D1 (de) 2000-08-17
EP0564178A1 (de) 1993-10-06
JPH07270307A (ja) 1995-10-20

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee