DE69133559D1 - Direkte Klonierung von PCR amplifizierten Nucleinsäuren - Google Patents

Direkte Klonierung von PCR amplifizierten Nucleinsäuren

Info

Publication number
DE69133559D1
DE69133559D1 DE69133559T DE69133559T DE69133559D1 DE 69133559 D1 DE69133559 D1 DE 69133559D1 DE 69133559 T DE69133559 T DE 69133559T DE 69133559 T DE69133559 T DE 69133559T DE 69133559 D1 DE69133559 D1 DE 69133559D1
Authority
DE
Germany
Prior art keywords
nucleic acids
pcr amplified
amplified nucleic
direct cloning
cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69133559T
Other languages
English (en)
Other versions
DE69133559T2 (de
Inventor
Corinna Hernstadt
Joseph M Fernandez
David A Mead
Lloyd Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of DE69133559D1 publication Critical patent/DE69133559D1/de
Application granted granted Critical
Publication of DE69133559T2 publication Critical patent/DE69133559T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69133559T 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nucleinsäuren Expired - Lifetime DE69133559T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58981790A 1990-09-27 1990-09-27
US589817 1990-09-27

Publications (2)

Publication Number Publication Date
DE69133559D1 true DE69133559D1 (de) 2007-03-22
DE69133559T2 DE69133559T2 (de) 2007-11-22

Family

ID=24359662

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69133389T Expired - Lifetime DE69133389T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nukleinsäuren
DE69133559T Expired - Lifetime DE69133559T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nucleinsäuren
DE69119083T Expired - Lifetime DE69119083T2 (de) 1990-09-27 1991-09-27 Direkte klonierung von pcr amplifizierten nukleinsäuren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69133389T Expired - Lifetime DE69133389T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nukleinsäuren

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69119083T Expired - Lifetime DE69119083T2 (de) 1990-09-27 1991-09-27 Direkte klonierung von pcr amplifizierten nukleinsäuren

Country Status (6)

Country Link
US (2) US5487993A (de)
EP (4) EP1790723A3 (de)
AU (1) AU8871891A (de)
DE (3) DE69133389T2 (de)
HK (1) HK1064124A1 (de)
WO (1) WO1992006189A1 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5426039A (en) * 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
US5994058A (en) * 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
AU3568197A (en) * 1996-06-18 1998-01-07 Novagen, Inc. Direct cloning of dna fragments
US5856144A (en) * 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments
US6140086A (en) * 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
NZ520579A (en) * 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
EP1141275B1 (de) * 1999-01-05 2009-08-12 Trustees Of Boston University Verbessertes klonierungsverfahren
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
JP2000253885A (ja) * 1999-03-11 2000-09-19 Jcr Pharmaceuticals Co Ltd 任意プライマーｐｃｒを用いるｄｎａ断片クローニング方法
JP2002539800A (ja) * 1999-03-24 2002-11-26 ジーン セラピー システムズ インコーポレーテッド 転写活性のあるｄｎａ断片を製造する方法
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
US6797509B1 (en) 1999-07-09 2004-09-28 Degussa-Huls Ag Nucleotide sequences which code for the tal gene
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
KR100453279B1 (ko) * 2000-04-27 2004-10-15 주식회사 서린바이오사이언스 양쪽 3'끝이 동일하거나 비상보적인 하나의 염기돌출부위를 갖는 벡터, ＥｃｌＨＫＩ 카세트 및 상기벡터와 ＥｃｌＨＫＩ 카세트의 이용방법
GB0012233D0 (en) 2000-05-19 2000-07-12 Devgen Nv Vector constructs
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE10109690A1 (de) 2000-09-02 2002-03-14 Degussa Neue für das metY-Gen kodierende Nukleotidsequenzen
US6544782B1 (en) 2000-11-13 2003-04-08 Synthegen Systems pREM: a positive selection vector system for direct PCR cloning
ATE460472T1 (de) 2000-12-08 2010-03-15 Life Technologies Corp Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen
US6566067B2 (en) * 2001-02-14 2003-05-20 Synthegen Systems, Inc. High fidelity PCR cloning
US7060434B2 (en) * 2001-02-14 2006-06-13 Council Of Scientific & Industrial Research Probes for myctophid fish and a method for developing the same
DE10133928A1 (de) * 2001-07-12 2003-01-23 Bayer Cropscience Ag Verfahren zum Herstellen von Deletionsmutanten
KR100441201B1 (ko) * 2001-09-10 2004-07-23 대한민국 리포터 유전자를 포함하는 티벡터용 플라스미드 및 그 제조방법
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
AU2002340234A1 (en) * 2001-10-12 2003-04-22 Modular Genetics, Inc. System for manipulating nucleic acids
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
AU2002257486A1 (en) * 2002-04-25 2003-11-10 Institute Of Zoology, Chinese Academy Of Sciences Method for constructing a linear expression vector without cloning
US6586237B1 (en) * 2002-05-24 2003-07-01 Kyle David Yesland Compositions and methods for cloning nucleic acids
US6803981B2 (en) * 2002-05-24 2004-10-12 Hannstar Display Corporation Liquid crystal display having biased bending vertical alignment
JP2005532829A (ja) * 2002-07-18 2005-11-04 インヴィトロジェン コーポレーション 組換え部位を含むウイルスベクター
WO2004057003A2 (de) 2002-12-20 2004-07-08 Metanomics Gmbh & Co. Kgaa Verfahren zur herstellung von aminosäuren mittels transgener organismen
US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
EP1606419A1 (de) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systeme und methoden zur erhohung der milchproduktion in tieren
EP1616033B1 (de) 2003-03-25 2010-03-03 Stratagene California Dna-polymerasefusionen und deren verwendungen
EP2434019A1 (de) 2003-08-01 2012-03-28 BASF Plant Science GmbH Verfahren zur Herstellung von Feinchemikalien
KR101077334B1 (ko) 2003-09-29 2011-10-26 몬산토 테크놀로지 엘엘씨 식물에서 스트레스 내성을 향상시키는 방법 및 이들의 방법
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
JP2007525217A (ja) 2004-02-19 2007-09-06 ザ ガバナーズ オブ ザ ユニバーシティー オブ アルバータ レプチンプロモーター多型及びその使用
CN100465278C (zh) * 2004-06-10 2009-03-04 清华大学 一种构建t载体的方法
US20050281740A1 (en) * 2004-06-16 2005-12-22 Glen Gong Imaging damaged lung tissue
CA2559760A1 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
MX2007007040A (es) 2004-12-17 2008-10-24 Metanomics Gmbh Proceso para el control de produccion de productos quimicos finos.
JP5420174B2 (ja) * 2004-12-23 2014-02-19 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ライゲーションによるｒｎａ増幅法
KR101233739B1 (ko) 2004-12-28 2013-02-18 아지노모토 가부시키가이샤 Ｌ-글루탐산 생산 미생물 및 ｌ-글루탐산의 제조방법
EP1841890B1 (de) * 2005-01-03 2013-08-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services Primer für nukleinsäurenachweis
DE102005047596A1 (de) 2005-10-05 2007-04-12 Degussa Ag Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
BRPI0617491B1 (pt) 2005-10-18 2021-04-27 Ajinomoto Co., Inc Processo para produção de ácido succínico
EP2025762A3 (de) 2006-01-17 2009-09-30 Health Research Inc. Heteroduplex-Trackingassay
JP2009531464A (ja) 2006-03-29 2009-09-03 メリアル リミテッド 連鎖球菌に対するワクチン
EP2090662A3 (de) 2006-04-05 2012-10-31 Metanomics GmbH Verfahren zur Herstellung einer feinen Chemikalie
US7723103B2 (en) * 2007-01-08 2010-05-25 Lucigen Corporation Vectors, kits and methods for cloning DNA
DE102007005072A1 (de) 2007-02-01 2008-08-07 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von Cadaverin
US7881933B2 (en) * 2007-03-23 2011-02-01 Verizon Patent And Licensing Inc. Age determination using speech
EP2149607B1 (de) 2007-04-10 2017-12-20 Ajinomoto Co., Inc. Verfahren zur herstellung von succinylsäure
AU2009321519A1 (en) * 2008-11-03 2011-06-30 Alchemy Biosciences Pty Ltd Method of cloning DNA
JP2012223091A (ja) 2009-08-25 2012-11-15 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
CN102741273A (zh) 2009-12-16 2012-10-17 华盛顿大学 毒素-免疫系统
US8557972B2 (en) * 2009-12-21 2013-10-15 University Of Washington Through Its Center For Commercialization Compositions and methods for transfection of RNA and controlled stabilization of transfected RNA
CA2825357C (en) 2011-02-24 2018-07-10 Inserm (Institut National De La Sante Et De La Recherche Medicale) Igfbp-3 derivatives and uses thereof
RU2507266C2 (ru) * 2011-11-16 2014-02-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") НЕТРАНСКРИБИРУЕМЫЙ ПЛАЗМИДНЫЙ ВЕКТОР pBL-T ДЛЯ КЛОНИРОВАНИЯ СЛОЖНЫХ ГЕНОМНЫХ ПОСЛЕДОВАТЕЛЬНОСТЕЙ И МНОГОМОДУЛЬНОЙ СБОРКИ ГЕННО-ИНЖЕНЕРНЫХ КОНСТРУКЦИЙ (ВАРИАНТЫ), И НАБОР, СОДЕРЖАЩИЙ УКАЗАННЫЙ ВЕКТОР
US10358655B1 (en) 2012-06-27 2019-07-23 University Of Utah Research Foundation Attenuated protein expression vectors and associated methods
DE102012016716A1 (de) 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2017049231A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN113604546A (zh) 2015-09-22 2021-11-05 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
KR20180084756A (ko) 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
KR102345899B1 (ko) 2016-06-30 2021-12-31 지머젠 인코포레이티드 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
EP3516528A4 (de) 2016-09-21 2020-06-24 Twist Bioscience Corporation Nukleinsäurebasierte datenspeicherung
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
CN107058357A (zh) * 2017-03-13 2017-08-18 四川农业大学 一种基于酶切的ta克隆载体及其构建方法和应用
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3635117A2 (de) 2017-06-07 2020-04-15 Zymergen, Inc. Promotoren aus corynebacterium glutamicum und deren verwendungen zur regulierung der expression von hilfsgenen
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3638782A4 (de) 2017-06-12 2021-03-17 Twist Bioscience Corporation Verfahren für nahtlose nukleinsäureanordnung
EP3681906A4 (de) 2017-09-11 2021-06-09 Twist Bioscience Corporation Gpcr-bindende proteine und deren synthese
KR20240024357A (ko) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
GB2585506A (en) 2018-01-04 2021-01-13 Twist Bioscience Corp DNA-based digital information storage
GB2590196A (en) 2018-05-18 2021-06-23 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
EP3938506A4 (de) 2019-02-26 2022-12-14 Twist Bioscience Corporation Variante nukleinsäurebibliotheken zur optimierung von antikörpern
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
WO2022167396A1 (en) 2021-02-02 2022-08-11 Biocopy Gmbh Method for identifying emerging pathogen mutations enabling host cell entry and immune evasion

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
AU3568197A (en) * 1996-06-18 1998-01-07 Novagen, Inc. Direct cloning of dna fragments
US5856144A (en) * 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments

Also Published As

Publication number Publication date
EP1396540A3 (de) 2004-06-09
EP1396540A2 (de) 2004-03-10
DE69133389D1 (de) 2004-06-24
US5827657A (en) 1998-10-27
DE69119083D1 (de) 1996-05-30
EP1790723A3 (de) 2008-10-22
AU8871891A (en) 1992-04-28
EP0738779A3 (de) 1997-05-07
EP0550693A1 (de) 1993-07-14
DE69119083T2 (de) 1996-10-17
EP0738779B1 (de) 2004-05-19
DE69133389T2 (de) 2005-06-02
EP0550693B1 (de) 1996-04-24
WO1992006189A1 (en) 1992-04-16
EP1396540B1 (de) 2007-02-07
HK1064124A1 (en) 2005-01-21
EP0738779A2 (de) 1996-10-23
EP0550693A4 (de) 1994-04-27
US5487993A (en) 1996-01-30
DE69133559T2 (de) 2007-11-22
EP1790723A2 (de) 2007-05-30

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