HK1064124A1 - Direct cloning of pcr amplified nucleic acids - Google Patents

Direct cloning of pcr amplified nucleic acids

Info

Publication number
HK1064124A1
HK1064124A1 HK04106903A HK04106903A HK1064124A1 HK 1064124 A1 HK1064124 A1 HK 1064124A1 HK 04106903 A HK04106903 A HK 04106903A HK 04106903 A HK04106903 A HK 04106903A HK 1064124 A1 HK1064124 A1 HK 1064124A1
Authority
HK
Hong Kong
Prior art keywords
nucleic acids
pcr amplified
amplified nucleic
direct cloning
cloning
Prior art date
Application number
HK04106903A
Other languages
English (en)
Inventor
Corinna Hernstadt
Joseph M Fernandez
David A Mead
Lloyd Smith
Original Assignee
Invitrogen Corp
Chimerx Subdivision Of Molecul
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp, Chimerx Subdivision Of Molecul filed Critical Invitrogen Corp
Publication of HK1064124A1 publication Critical patent/HK1064124A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
HK04106903A 1990-09-27 2004-09-10 Direct cloning of pcr amplified nucleic acids HK1064124A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US58981790A 1990-09-27 1990-09-27

Publications (1)

Publication Number Publication Date
HK1064124A1 true HK1064124A1 (en) 2005-01-21

Family

ID=24359662

Family Applications (1)

Application Number Title Priority Date Filing Date
HK04106903A HK1064124A1 (en) 1990-09-27 2004-09-10 Direct cloning of pcr amplified nucleic acids

Country Status (6)

Country Link
US (2) US5487993A (de)
EP (4) EP1790723A3 (de)
AU (1) AU8871891A (de)
DE (3) DE69119083T2 (de)
HK (1) HK1064124A1 (de)
WO (1) WO1992006189A1 (de)

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Also Published As

Publication number Publication date
EP1790723A2 (de) 2007-05-30
US5487993A (en) 1996-01-30
EP0550693A1 (de) 1993-07-14
US5827657A (en) 1998-10-27
EP0738779A3 (de) 1997-05-07
DE69133559T2 (de) 2007-11-22
DE69133389D1 (de) 2004-06-24
EP0550693B1 (de) 1996-04-24
EP1396540B1 (de) 2007-02-07
WO1992006189A1 (en) 1992-04-16
EP0738779B1 (de) 2004-05-19
DE69133389T2 (de) 2005-06-02
EP1396540A3 (de) 2004-06-09
AU8871891A (en) 1992-04-28
EP1396540A2 (de) 2004-03-10
DE69119083T2 (de) 1996-10-17
EP0550693A4 (de) 1994-04-27
EP0738779A2 (de) 1996-10-23
EP1790723A3 (de) 2008-10-22
DE69133559D1 (de) 2007-03-22
DE69119083D1 (de) 1996-05-30

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