AU3568197A - Direct cloning of dna fragments - Google Patents

Direct cloning of dna fragments

Info

Publication number
AU3568197A
AU3568197A AU35681/97A AU3568197A AU3568197A AU 3568197 A AU3568197 A AU 3568197A AU 35681/97 A AU35681/97 A AU 35681/97A AU 3568197 A AU3568197 A AU 3568197A AU 3568197 A AU3568197 A AU 3568197A
Authority
AU
Australia
Prior art keywords
dna fragments
direct cloning
cloning
direct
fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU35681/97A
Inventor
Kristin M. Kolb
Robert C. Mierendorf
Robert E. Novy
David O'reilly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novagen Inc
Original Assignee
Novagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9612713.9A external-priority patent/GB9612713D0/en
Priority claimed from GBGB9614765.7A external-priority patent/GB9614765D0/en
Application filed by Novagen Inc filed Critical Novagen Inc
Publication of AU3568197A publication Critical patent/AU3568197A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU35681/97A 1996-06-18 1997-06-18 Direct cloning of dna fragments Abandoned AU3568197A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9612713.9A GB9612713D0 (en) 1996-06-18 1996-06-18 U-vector cloning
GB9612713 1996-06-18
GBGB9614765.7A GB9614765D0 (en) 1996-07-13 1996-07-13 Direct cloning of mononucleotide-tailed DNA fragments
GB9614765 1996-07-13
PCT/US1997/009696 WO1997048791A1 (en) 1996-06-18 1997-06-18 Direct cloning of dna fragments

Publications (1)

Publication Number Publication Date
AU3568197A true AU3568197A (en) 1998-01-07

Family

ID=26309533

Family Applications (1)

Application Number Title Priority Date Filing Date
AU35681/97A Abandoned AU3568197A (en) 1996-06-18 1997-06-18 Direct cloning of dna fragments

Country Status (2)

Country Link
AU (1) AU3568197A (en)
WO (1) WO1997048791A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0550693B1 (en) * 1990-09-27 1996-04-24 Invitrogen Corporation Direct cloning of pcr amplified nucleic acids

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0550693B1 (en) * 1990-09-27 1996-04-24 Invitrogen Corporation Direct cloning of pcr amplified nucleic acids

Also Published As

Publication number Publication date
WO1997048791A1 (en) 1997-12-24

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