DE60304665D1 - Vorrichtung und Verfahren zur Analyse einer Vielzahl fluoreszierender Farbstoffe mittels einer Vielzahl von Photodetektoren mit regelbarer Verstärkung - Google Patents

Vorrichtung und Verfahren zur Analyse einer Vielzahl fluoreszierender Farbstoffe mittels einer Vielzahl von Photodetektoren mit regelbarer Verstärkung

Info

Publication number
DE60304665D1
DE60304665D1 DE60304665T DE60304665T DE60304665D1 DE 60304665 D1 DE60304665 D1 DE 60304665D1 DE 60304665 T DE60304665 T DE 60304665T DE 60304665 T DE60304665 T DE 60304665T DE 60304665 D1 DE60304665 D1 DE 60304665D1
Authority
DE
Germany
Prior art keywords
fluorescent dyes
photodetectors
instrument
analyzing
variable gain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60304665T
Other languages
English (en)
Other versions
DE60304665T2 (de
Inventor
E Bishop
Sunil S Dalal
Michael D Lock
Zhenxiang Gong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of DE60304665D1 publication Critical patent/DE60304665D1/de
Application granted granted Critical
Publication of DE60304665T2 publication Critical patent/DE60304665T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/124—Sensitivity
    • G01N2201/1241—Multirange

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE60304665T 2002-12-20 2003-11-27 Vorrichtung und Verfahren zur Analyse einer Vielzahl fluoreszierender Farbstoffe mittels einer Vielzahl von Photodetektoren mit regelbarer Verstärkung Expired - Lifetime DE60304665T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/326,524 US6897954B2 (en) 2002-12-20 2002-12-20 Instrument setup system for a fluorescence analyzer
US326524 2002-12-20

Publications (2)

Publication Number Publication Date
DE60304665D1 true DE60304665D1 (de) 2006-05-24
DE60304665T2 DE60304665T2 (de) 2007-02-15

Family

ID=32393129

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60304665T Expired - Lifetime DE60304665T2 (de) 2002-12-20 2003-11-27 Vorrichtung und Verfahren zur Analyse einer Vielzahl fluoreszierender Farbstoffe mittels einer Vielzahl von Photodetektoren mit regelbarer Verstärkung

Country Status (5)

Country Link
US (1) US6897954B2 (de)
EP (1) EP1431745B1 (de)
JP (1) JP4580166B2 (de)
AT (1) ATE323884T1 (de)
DE (1) DE60304665T2 (de)

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Also Published As

Publication number Publication date
ATE323884T1 (de) 2006-05-15
US6897954B2 (en) 2005-05-24
JP2004205508A (ja) 2004-07-22
DE60304665T2 (de) 2007-02-15
EP1431745A1 (de) 2004-06-23
US20040119974A1 (en) 2004-06-24
EP1431745B1 (de) 2006-04-19
JP4580166B2 (ja) 2010-11-10

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