DE602004023128D1 - Neue expressionswerkzeuge für multiproteinanwendungen - Google Patents

Neue expressionswerkzeuge für multiproteinanwendungen

Info

Publication number
DE602004023128D1
DE602004023128D1 DE602004023128T DE602004023128T DE602004023128D1 DE 602004023128 D1 DE602004023128 D1 DE 602004023128D1 DE 602004023128 T DE602004023128 T DE 602004023128T DE 602004023128 T DE602004023128 T DE 602004023128T DE 602004023128 D1 DE602004023128 D1 DE 602004023128D1
Authority
DE
Germany
Prior art keywords
multiprotein
applications
new expression
host cells
expression tools
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004023128T
Other languages
English (en)
Inventor
Imre Berger
Daniel J Fitzgerald
Timothy Richmond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eidgenoessische Technische Hochschule Zurich ETHZ
Original Assignee
Eidgenoessische Technische Hochschule Zurich ETHZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eidgenoessische Technische Hochschule Zurich ETHZ filed Critical Eidgenoessische Technische Hochschule Zurich ETHZ
Publication of DE602004023128D1 publication Critical patent/DE602004023128D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/10—Drugs for disorders of the endocrine system of the posterior pituitary hormones, e.g. oxytocin, ADH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE602004023128T 2004-03-09 2004-11-25 Neue expressionswerkzeuge für multiproteinanwendungen Active DE602004023128D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55207204P 2004-03-09 2004-03-09
PCT/EP2004/013381 WO2005085456A1 (en) 2004-03-09 2004-11-25 New expression tools for multiprotein applications

Publications (1)

Publication Number Publication Date
DE602004023128D1 true DE602004023128D1 (de) 2009-10-22

Family

ID=34919595

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004023128T Active DE602004023128D1 (de) 2004-03-09 2004-11-25 Neue expressionswerkzeuge für multiproteinanwendungen

Country Status (9)

Country Link
US (2) US20080004228A1 (de)
EP (2) EP2133428A1 (de)
JP (1) JP4597188B2 (de)
AT (1) ATE442450T1 (de)
AU (1) AU2004316874B2 (de)
CA (1) CA2558313C (de)
DE (1) DE602004023128D1 (de)
ES (1) ES2333228T3 (de)
WO (1) WO2005085456A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
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RU2478709C2 (ru) 2005-07-21 2013-04-10 Эбботт Лэборетриз ЭКСПРЕССИЯ МНОЖЕСТВА ГЕНОВ, ВКЛЮЧАЯ sORF-КОНСТРУКЦИИ, И СПОСОБЫ ЭКСПРЕССИРОВАНИЯ ИММУНОГЛОБУЛИНА
EP1783228A1 (de) * 2005-11-08 2007-05-09 ETH Zürich Rekombinante Expression von Multiproteinkomplexen mit Hilfe von Multigenen
JP5152962B2 (ja) * 2007-06-08 2013-02-27 国立大学法人静岡大学 目的タンパク質の製造方法
GB0820631D0 (en) 2008-11-11 2008-12-17 London School Hygiene & Tropical Medicine Vectors
US8709798B2 (en) 2009-03-06 2014-04-29 Europaisches Laboratorium Fur Molekularbiologie Nucleic acids for cloning and expressing multiprotein complexes
WO2010112533A1 (en) 2009-04-03 2010-10-07 Deutsches Krebsforschungszentrum Enhanced production of papillomavirus-like particles with a modified baculovirus expression system
CN102414313B (zh) * 2009-05-01 2014-04-02 雷德生物科技股份公司 多基因载体编码的重组病毒样颗粒
CN101947317B (zh) * 2010-09-27 2012-05-30 武汉大学 一种h5n1禽流感病毒样颗粒疫苗的规模化制备方法
FR2978456B1 (fr) 2011-07-27 2015-02-20 Genethon Systeme baculoviral pour l'expression d'un vecteur de therapie genique
WO2013078433A1 (en) 2011-11-23 2013-05-30 University Of Hawaii Auto-processing domains for polypeptide expression
WO2013100713A2 (ko) * 2011-12-30 2013-07-04 이화여자대학교 산학협력단 효모 염색체 통합 또는 산업 스트레인 형질전환용 효모 발현 벡터, 그리고 이들의 용도
KR101328481B1 (ko) * 2011-12-30 2013-11-13 이화여자대학교 산학협력단 염색체 통합용 효모 발현 벡터 및 이의 용도
ES2612854T3 (es) * 2012-06-12 2017-05-19 Alternative Gene Expression, S.L. Elementos de ADN recombinante para la expresión de proteínas recombinantes en una célula huésped
RU2619161C2 (ru) 2012-06-12 2017-05-12 Альтернативе Гене Экспрессион С.Л. Элементы рекомбинантной днк для экспрессии рекомбинантных белков в клетке-хозяине
CN105392362B (zh) 2012-06-12 2018-11-09 替代基因表达公司 用于在宿主昆虫中表达重组蛋白的杆状病毒dna元件
EP2970989A1 (de) * 2013-03-14 2016-01-20 Friedrich Miescher Institute for Biomedical Research Verfahren zur herstellung von polycystronischen vektoren
CA2973943A1 (en) * 2015-01-16 2016-07-21 National Institute Of Advanced Industrial Science And Technology Gene expression system using stealthy rna, and gene introduction/expression vector including said rna
CN104630271A (zh) * 2015-02-05 2015-05-20 浙江大学 可视化红色荧光杆状病毒的构建方法
US10611800B2 (en) 2016-03-11 2020-04-07 Pfizer Inc. Human cytomegalovirus gB polypeptide
US10262981B2 (en) * 2016-04-29 2019-04-16 Taiwan Semiconductor Manufacturing Company, Ltd. Integrated circuit, system for and method of forming an integrated circuit
CN107988259B (zh) * 2018-01-12 2020-01-21 中国科学院生物物理研究所 SmartBac杆状病毒表达系统及其应用
US20210332088A1 (en) 2018-07-31 2021-10-28 Imophoron Limited Multimerizing Polypeptides Derived From Jelly Roll Fold Domain of Adenovirus Penton Base
US11629172B2 (en) 2018-12-21 2023-04-18 Pfizer Inc. Human cytomegalovirus gB polypeptide
US20200407696A1 (en) * 2019-06-26 2020-12-31 Haifeng Chen Baculovirus expression system
WO2021110993A1 (en) * 2019-12-04 2021-06-10 Synbionik Gmbh An efficient shuttle vector system for the expression of heterologous and homologous proteins for the genus zymomonas
BR112022015461A2 (pt) 2020-02-05 2022-10-11 Imophoron Ltd Polipeptídeos, ácido nucleico, vetor, célula hospedeira, proteínas de base do penton de adenovírus, complexo pentamérico, partícula semelhante a vírus (vlp), composição farmacêutica, métodos para produzir a vlp e para a produção de um polipeptídeo e método de identificação de uma sequência de ligação
CN111593072B (zh) * 2020-05-12 2021-04-27 深圳海关动植物检验检疫技术中心 一种在昆虫细胞中共表达非洲猪瘟病毒四种结构蛋白的方法及其应用
EP4168426A1 (de) 2020-06-18 2023-04-26 The University of Bristol Fettsäurekomplexe des coronavirus-spike-proteins und deren verwendung
US11857622B2 (en) 2020-06-21 2024-01-02 Pfizer Inc. Human cytomegalovirus GB polypeptide
KR20240049821A (ko) * 2021-08-23 2024-04-17 바이오버라티브 테라퓨틱스 인크. 바큘로바이러스 발현 시스템

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US5169784A (en) * 1990-09-17 1992-12-08 The Texas A & M University System Baculovirus dual promoter expression vector
US5871986A (en) * 1994-09-23 1999-02-16 The General Hospital Corporation Use of a baculovirus to express and exogenous gene in a mammalian cell
US6355240B1 (en) * 1995-07-26 2002-03-12 Basf Aktiengellschaft Enhanced insecticidal insect virus through the expression of heterologous proteins with early promoters
AU2002251935B2 (en) * 2001-02-14 2007-11-29 Celularity Inc. Post-partum mammalian placenta, its use and placental stem cells therefrom
AU2003253992A1 (en) * 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites

Also Published As

Publication number Publication date
AU2004316874A1 (en) 2005-09-15
AU2004316874B2 (en) 2011-01-06
CA2558313A1 (en) 2005-09-15
US20160177336A1 (en) 2016-06-23
ATE442450T1 (de) 2009-09-15
WO2005085456A1 (en) 2005-09-15
CA2558313C (en) 2011-05-17
EP2133428A1 (de) 2009-12-16
EP1723246A1 (de) 2006-11-22
JP4597188B2 (ja) 2010-12-15
JP2007527722A (ja) 2007-10-04
EP1723246B1 (de) 2009-09-09
ES2333228T3 (es) 2010-02-18
US20080004228A1 (en) 2008-01-03

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