WO2006090282A3 - Recombinant inhibitor proteins of an hk14 protease and use thereof - Google Patents

Recombinant inhibitor proteins of an hk14 protease and use thereof Download PDF

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Publication number
WO2006090282A3
WO2006090282A3 PCT/IB2006/000574 IB2006000574W WO2006090282A3 WO 2006090282 A3 WO2006090282 A3 WO 2006090282A3 IB 2006000574 W IB2006000574 W IB 2006000574W WO 2006090282 A3 WO2006090282 A3 WO 2006090282A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
recombinant inhibitor
inhibitor protein
purified
nucleic acid
Prior art date
Application number
PCT/IB2006/000574
Other languages
French (fr)
Other versions
WO2006090282A2 (en
Inventor
David Deperthes
Christoph Kuendig
Sylvain Cloutier
Loyse Felber
Original Assignee
Univ Lausanne
David Deperthes
Christoph Kuendig
Sylvain Cloutier
Loyse Felber
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Lausanne, David Deperthes, Christoph Kuendig, Sylvain Cloutier, Loyse Felber filed Critical Univ Lausanne
Publication of WO2006090282A2 publication Critical patent/WO2006090282A2/en
Publication of WO2006090282A3 publication Critical patent/WO2006090282A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a recombinant inhibitor protein of an hK14 protease comprising a Reactive Serpin Loop of a serpin sequence which is modified by at least one substrate active site sequence specific for said hK14 protease. Other objects of the invention are to provide a purified and isolated nucleic acid sequence encoding the recombinant inhibitor protein of said hKl 4 protease, an expression vector comprising said purified and isolated nucleic acid sequence, a eukaryotic or prokaryotic host cell transformed with this expression vector and a method of producing a recombinant inhibitor protein of an hK14 protease.
PCT/IB2006/000574 2005-02-28 2006-02-28 Recombinant inhibitor proteins of an hk14 protease and use thereof WO2006090282A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IBPCT/IB2005/000504 2005-02-28
IB2005000504 2005-02-28

Publications (2)

Publication Number Publication Date
WO2006090282A2 WO2006090282A2 (en) 2006-08-31
WO2006090282A3 true WO2006090282A3 (en) 2007-04-12

Family

ID=36927802

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2006/000574 WO2006090282A2 (en) 2005-02-28 2006-02-28 Recombinant inhibitor proteins of an hk14 protease and use thereof

Country Status (1)

Country Link
WO (1) WO2006090282A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009093119A2 (en) * 2008-01-21 2009-07-30 Med Discovery S.A. Use of serine protease inhibitors in the treatment of skin diseases
JP5667094B2 (en) * 2009-03-10 2015-02-12 エムイーディー ディスカバリー エスエー Use of serine protease inhibitors in the treatment of neutropenia
GB201322091D0 (en) * 2013-12-13 2014-01-29 Cambridge Entpr Ltd Modified serpins for the treatment of bleeding disorders
WO2018154044A1 (en) 2017-02-23 2018-08-30 Umc Utrecht Holding B.V. Modified serpins for the treatment of bradykinin-mediated disease
BR102017005783A2 (en) * 2017-03-21 2018-10-02 Fund Butantan process for obtaining esculptin and fragments thereof, recombinant sculptin and its uses

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029285A2 (en) * 2002-09-26 2004-04-08 Mount Sinai Hospital Methods for detecting endocrine cancer
WO2004087912A1 (en) * 2003-04-04 2004-10-14 Université de Lausanne Inhibitor proteins of a protease and use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029285A2 (en) * 2002-09-26 2004-04-08 Mount Sinai Hospital Methods for detecting endocrine cancer
WO2004087912A1 (en) * 2003-04-04 2004-10-14 Université de Lausanne Inhibitor proteins of a protease and use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CLOUTIER SYLVAIN M ET AL: "Development of recombinant inhibitors specific to human kallikrein 2 using phage-display selected substrates", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 271, no. 3, February 2004 (2004-02-01), pages 607 - 613, XP002357848, ISSN: 0014-2956 *
DUFOUR ERICK K ET AL: "The contribution of arginine residues within the P6-P1 region of alpha1-antitrypsin to its reaction with furin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 42, 19 October 2001 (2001-10-19), pages 38971 - 38979, XP002414625, ISSN: 0021-9258 *
FELBER LOYSE M ET AL: "Mutant recombinant serpins as highly specific inhibitors of human kallikrein 14", FEBS JOURNAL, vol. 273, no. 11, June 2006 (2006-06-01), pages 2505 - 2514, XP002414626, ISSN: 1742-464X(print) 1742-4658(ele *
HOOPER J D ET AL: "Identification and Characterization of KLK14, a Novel Kallikrein Serine Protease Gene Located on Human Chromosome 19q13.4 and Expressed in Prostate and Skeletal Muscle", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 73, no. 1, 1 April 2001 (2001-04-01), pages 117 - 122, XP004432260, ISSN: 0888-7543 *

Also Published As

Publication number Publication date
WO2006090282A2 (en) 2006-08-31

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