EA200971142A1 - Система экспрессии гетерологичных и гомологичных целлюлаз - Google Patents

Система экспрессии гетерологичных и гомологичных целлюлаз

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Publication number
EA200971142A1
EA200971142A1 EA200971142A EA200971142A EA200971142A1 EA 200971142 A1 EA200971142 A1 EA 200971142A1 EA 200971142 A EA200971142 A EA 200971142A EA 200971142 A EA200971142 A EA 200971142A EA 200971142 A1 EA200971142 A1 EA 200971142A1
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EA
Eurasian Patent Office
Prior art keywords
heterological
homological
cellulases
expression
heterologous
Prior art date
Application number
EA200971142A
Other languages
English (en)
Other versions
EA018049B1 (ru
Inventor
Бенджамин С. Бауэр
Эдмунд А. Ларенас
Original Assignee
ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН filed Critical ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН
Publication of EA200971142A1 publication Critical patent/EA200971142A1/ru
Publication of EA018049B1 publication Critical patent/EA018049B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к нитевидным грибам, экспрессирующим комбинацию гетерологичных и гомологичных полипептидов, к полипептидным смесям, включающим комбинацию гетерологичных и гомологичных полипептидов, и к способам получения полипептидных смесей.
EA200971142A 2007-06-08 2008-06-05 Система экспрессии гетерологичных и гомологичных целлюлаз EA018049B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93389407P 2007-06-08 2007-06-08
PCT/US2008/007077 WO2008153903A2 (en) 2007-06-08 2008-06-05 Heterologous and homologous cellulase expression system

Publications (2)

Publication Number Publication Date
EA200971142A1 true EA200971142A1 (ru) 2010-04-30
EA018049B1 EA018049B1 (ru) 2013-05-30

Family

ID=39767012

Family Applications (2)

Application Number Title Priority Date Filing Date
EA200971142A EA018049B1 (ru) 2007-06-08 2008-06-05 Система экспрессии гетерологичных и гомологичных целлюлаз
EA201300048A EA201300048A1 (ru) 2007-06-08 2008-06-05 Система экспрессии гетерологичных и гомологичных целлюлаз

Family Applications After (1)

Application Number Title Priority Date Filing Date
EA201300048A EA201300048A1 (ru) 2007-06-08 2008-06-05 Система экспрессии гетерологичных и гомологичных целлюлаз

Country Status (12)

Country Link
US (2) US20100323426A1 (ru)
EP (1) EP2171039A2 (ru)
JP (1) JP5651466B2 (ru)
CN (2) CN104726350A (ru)
BR (1) BRPI0812920A2 (ru)
CA (1) CA2693084A1 (ru)
EA (2) EA018049B1 (ru)
HK (1) HK1145851A1 (ru)
IL (1) IL202304A (ru)
MX (2) MX2009013329A (ru)
UA (1) UA104279C2 (ru)
WO (1) WO2008153903A2 (ru)

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EA018049B1 (ru) * 2007-06-08 2013-05-30 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Система экспрессии гетерологичных и гомологичных целлюлаз
DK2450438T3 (en) * 2009-07-03 2015-12-07 Meiji Seika Pharma Co Ltd The cellulase composition containing endoglucanases from two different types of micro-organisms
MX2012003091A (es) 2009-09-23 2012-04-30 Danisco Us Inc Enzimas de glicosil hidrolasa novedosas y usos de las mismas.
CN102686736B (zh) 2009-12-23 2017-05-31 丹尼斯科美国公司 提高同步糖化发酵反应效率的方法
CA2789301A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
US8759064B2 (en) 2010-05-14 2014-06-24 Codexis, Inc. Cellobiohydrolase variants
MX2013010512A (es) 2011-03-17 2013-10-07 Danisco Inc Metodo para reducir la viscosidad en un proceso de sacarificacion.
EP2702154A4 (en) * 2011-04-27 2015-04-08 Codexis Inc VARIANTS OF CELLOBIOHYDROLASE
EP2748187A4 (en) 2011-08-23 2015-03-04 Codexis Inc VARIANTS OF CELLOBIOHYDROLASE
CN102304540B (zh) * 2011-08-26 2013-10-09 华东理工大学 一种在里氏木霉分泌表达外源蛋白的表达设备及其应用
WO2013043317A1 (en) * 2011-09-20 2013-03-28 Codexis, Inc. Endoglucanase 1b
RU2642290C2 (ru) 2011-09-22 2018-01-24 ДАНИСКО ЮЭс ИНК. Применение активности эндогенной днказы для понижения содержания днк
EP2852677A1 (en) 2012-05-21 2015-04-01 Danisco US Inc. Trichoderma hydrophobin production
WO2014192647A1 (ja) * 2013-05-27 2014-12-04 独立行政法人産業技術総合研究所 培養細胞および糖液の製造方法
BR112018015626A2 (pt) 2016-02-22 2018-12-26 Danisco Us Inc sistema fúngico de produção de alto nível de proteínas
DK3419991T3 (da) 2016-03-04 2023-01-16 Danisco Us Inc Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer
US10428334B2 (en) * 2016-04-05 2019-10-01 Wisconsin Alumni Research Foundation Method of creating industrial streptomyces with capability to grow on cellulosic polysaccharide substrates
EP3690040A4 (en) * 2017-09-25 2021-09-29 Ajinomoto Co., Inc. PROCESS FOR THE PRODUCTION OF PROTEIN AND PROCESS FOR THE PRODUCTION OF DISACCHARIDES
JP2021035330A (ja) * 2017-09-29 2021-03-04 Ｓｐｉｂｅｒ株式会社 発現カセット
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes

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Also Published As

Publication number Publication date
CN104726350A (zh) 2015-06-24
CA2693084A1 (en) 2008-12-18
JP5651466B2 (ja) 2015-01-14
WO2008153903A2 (en) 2008-12-18
WO2008153903A3 (en) 2009-03-05
MX344810B (es) 2017-01-09
IL202304A (en) 2016-08-31
IL202304A0 (en) 2011-08-01
CN101772570A (zh) 2010-07-07
MX2009013329A (es) 2010-02-24
US20100184138A1 (en) 2010-07-22
EP2171039A2 (en) 2010-04-07
BRPI0812920A2 (pt) 2014-10-14
US20100323426A1 (en) 2010-12-23
UA104279C2 (ru) 2014-01-27
EA201300048A1 (ru) 2013-09-30
JP2010528655A (ja) 2010-08-26
EA018049B1 (ru) 2013-05-30
HK1145851A1 (en) 2011-05-06
CN101772570B (zh) 2015-03-18

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