BR112018015626A2 - sistema fúngico de produção de alto nível de proteínas - Google Patents

sistema fúngico de produção de alto nível de proteínas

Info

Publication number
BR112018015626A2
BR112018015626A2 BR112018015626A BR112018015626A BR112018015626A2 BR 112018015626 A2 BR112018015626 A2 BR 112018015626A2 BR 112018015626 A BR112018015626 A BR 112018015626A BR 112018015626 A BR112018015626 A BR 112018015626A BR 112018015626 A2 BR112018015626 A2 BR 112018015626A2
Authority
BR
Brazil
Prior art keywords
high level
protein production
production system
fungal protein
level fungal
Prior art date
Application number
BR112018015626A
Other languages
English (en)
Inventor
Bianca Michielse Carola
Atalay Van Der Burgt Ismail
Visser Jacob
Meijnen Jean-Paul
Heinrich Visser Johannes
Alexander Bronkhof Jurian
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of BR112018015626A2 publication Critical patent/BR112018015626A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)

Abstract

são proporcionados células que têm uma produção proteica aumentada caracterizadas pelo fato de que a referida célula compreende sumoilação modificada, um processo para a produção de uma tal célula ou sistema de expressão e o uso de uma tal célula na produção de uma proteína de interesse.
BR112018015626A 2016-02-22 2017-02-22 sistema fúngico de produção de alto nível de proteínas BR112018015626A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662298351P 2016-02-22 2016-02-22
PCT/US2017/018901 WO2017147163A1 (en) 2016-02-22 2017-02-22 Fungal high-level protein production system

Publications (1)

Publication Number Publication Date
BR112018015626A2 true BR112018015626A2 (pt) 2018-12-26

Family

ID=58547785

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018015626A BR112018015626A2 (pt) 2016-02-22 2017-02-22 sistema fúngico de produção de alto nível de proteínas

Country Status (7)

Country Link
US (1) US10072267B2 (pt)
EP (1) EP3419992B1 (pt)
CN (1) CN109661402B (pt)
BR (1) BR112018015626A2 (pt)
DK (1) DK3419992T3 (pt)
ES (1) ES2855728T3 (pt)
WO (1) WO2017147163A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017021872A2 (pt) 2015-04-10 2018-07-10 Comet Biorefining Inc métodos e composições para o tratamento de biomassa celulósica e produtos obtidos da mesma
EP3790409A4 (en) 2018-05-10 2021-07-21 Comet Biorefining Inc. COMPOSITIONS WITH GLUCOSE AND HEMICELLULOSE AND THEIR USES
CN111748505B (zh) * 2019-03-28 2022-12-30 上海医药工业研究院 一种表达羧肽酶g2的基因工程菌及其制备方法和应用
CN110484455B (zh) * 2019-06-10 2022-05-24 潍坊康地恩生物科技有限公司 一种稳定高产植酸酶的木霉突变菌株

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
DK1117808T3 (da) 1998-10-06 2005-04-25 Mark Aaron Emalfarb Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium
JP2003180365A (ja) 2001-12-17 2003-07-02 National Research Inst Of Brewing 麹菌の固体培養時に発現する遺伝子の検出法
CN1688198A (zh) 2002-09-10 2005-10-26 金克克国际有限公司 使用高浓度糖混合物诱导基因表达
EP2109673B1 (en) * 2006-12-29 2015-03-11 Lifesensors, Inc. Methods and compositions for enhanced protein expression and purification
CA2693084A1 (en) 2007-06-08 2008-12-18 Benjamin S. Bower Heterologous and homologous cellulase expression system
CN102421895B (zh) 2009-03-16 2016-08-03 二进国际(美国)有限公司 Chrysosporium lucknowense蛋白生产系统
KR101181566B1 (ko) 2009-08-26 2012-09-10 성균관대학교산학협력단 ＳＵＭＯ-ｉｎｔｅｒａｃｔｉｎｇ ｍｏｔｉｆ (ＳＩＭ)를 포함하는 단백질들의 ＳＵＭＯ ｍｏｄｉｆｉｃａｔｉｏｎ을 증가시키는 ＳＵＭＯ-Ｕｂｃ9 융합 단백질
HUE028981T2 (en) 2010-11-02 2017-02-28 Codexis Inc Preparations and methods for preparing fermentable sugars
AU2012229042B2 (en) 2011-03-17 2017-03-02 Danisco Us Inc. Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
US20140073017A1 (en) 2011-03-17 2014-03-13 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
EP2791315A1 (en) 2011-12-14 2014-10-22 Iogen Energy Corporation Fungal cells and fermentation processes
EP2812429B1 (en) 2012-02-07 2020-07-29 Danisco US Inc. Glycosylation as a stabilizer for phytase
EP2838989A4 (en) 2012-04-19 2015-12-09 Dyadic Internat Usa Inc METHOD FOR ENHANCING ACTIVITY OF MIXTURES OF ENZYMES CELLULASES IN THE SACCHARIFICATION OF CELLULOSIC MATERIAL (LIGNO)
WO2014047520A1 (en) 2012-09-20 2014-03-27 Danisco Us Inc. Microtiter plates for controlled release of culture components to cell cultures
WO2014081700A1 (en) * 2012-11-20 2014-05-30 Codexis, Inc. Recombinant fungal polypeptides
BR112015029081A2 (pt) 2013-05-20 2017-09-19 Abengoa Bioenergia Nuevas Tecnologias Sa Célula hospedeira myceliophthora thermophila expressando uma enzima beta-xilosidase recombinante, enzima beta-xilosidase recombinante expressada por meio da mesma, composição, uso de célula hospedeira, enzima beta-xilosidase ou composição e métodos de produção de açúcares fermentescíveis e de produção de bioproduto a partir de biomassa
JP6814142B2 (ja) 2014-12-16 2021-01-13 ダニスコ・ユーエス・インク 真菌ゲノム改変システムおよび使用方法

Also Published As

Publication number Publication date
ES2855728T3 (es) 2021-09-24
DK3419992T3 (da) 2021-02-15
EP3419992A1 (en) 2019-01-02
US10072267B2 (en) 2018-09-11
US20170240909A1 (en) 2017-08-24
CN109661402A (zh) 2019-04-19
EP3419992B1 (en) 2020-11-18
CN109661402B (zh) 2022-06-28
WO2017147163A1 (en) 2017-08-31

Similar Documents

Publication Publication Date Title
BR112019007369A2 (pt) anticorpos anti-lag-3 e métodos de uso dos mesmos
CO2019006485A2 (es) Anticuerpos y metodos de su utilizacion
EA201992795A1 (ru) Способ получения эукариотических клеток с отредактированной днк и набор, используемый в этом способе
CO2020003882A2 (es) Anticuerpo multiespecifico
PE20210107A1 (es) Anticuerpos anti-cd3 y metodos de uso
BR112017009728A2 (pt) anticorpos isolados, ácido nucleico isolado, vetor, célula hospedeira, método para produzir o anticorpo, composição, usos de um anticorpo e métodos de tratamento de um distúrbio
MX2017006127A (es) Metodos y composiciones para produccion de proteina de clara de huevo.
BR112018074463A2 (pt) anticorpos anti-tim-3 e métodos de uso dos mesmos.
BR112017016897A2 (pt) cepas fúngicas e métodos de uso
CO2018002196A2 (es) Proteínas de fusión de factor ix y métodos para producirlas y utilizarlas
BR112017020054A2 (pt) anticorpos para icos
BR112018003186A2 (pt) anticorpos anti-pd-1 e seus métodos de uso
AR107078A1 (es) Anticuerpo antimiostatina, polipéptidos que contienen regiones fc variantes así como métodos de uso
UY37758A (es) Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos
BR112018015626A2 (pt) sistema fúngico de produção de alto nível de proteínas
BR112017013956B8 (pt) Proteínas de fusão de citocinas
IL254097B (en) Sub-pixel alignment of test to design
BR112016007251A2 (pt) método de processamento de biomassa que contém celulose
AR090319A1 (es) Biorrefineria integrada
AR105910A1 (es) Matrices de glicano y método de uso
BR112018010605A2 (pt) nanoemulsões de bebidas produzidas por processamento de alto cisalhamento
BR112016008477A2 (pt) Corpos, ácido nucleico, célula hospedeira, método de produção de um anticorpo, imunoconjugado, formulação farmacêutica e usos do anticorpo
BR112019003361A2 (pt) métodos e usos de frações de material orgânico dissolvido para ligação a íons metálicos
BR112018004474A2 (pt) posicionamento de e911 por intermédio de rastreamento de sinal de fundo
BR112012014809A2 (pt) método para produzir lentes de contato estabilizadas

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]