DE60123626D1 - Verfahren zur konstruktion von selbstassembierenden sonden und verfahren zu ihrer detektion - Google Patents

Verfahren zur konstruktion von selbstassembierenden sonden und verfahren zu ihrer detektion

Info

Publication number
DE60123626D1
DE60123626D1 DE60123626T DE60123626T DE60123626D1 DE 60123626 D1 DE60123626 D1 DE 60123626D1 DE 60123626 T DE60123626 T DE 60123626T DE 60123626 T DE60123626 T DE 60123626T DE 60123626 D1 DE60123626 D1 DE 60123626D1
Authority
DE
Germany
Prior art keywords
probes
self
regions
oligonucleotides
forming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60123626T
Other languages
English (en)
Other versions
DE60123626T2 (de
Inventor
Mitsugu Usui
Mari Mitsuka
Chikako Hakii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanko Junyaku Co Ltd
Original Assignee
Sanko Junyaku Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanko Junyaku Co Ltd filed Critical Sanko Junyaku Co Ltd
Publication of DE60123626D1 publication Critical patent/DE60123626D1/de
Application granted granted Critical
Publication of DE60123626T2 publication Critical patent/DE60123626T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
DE60123626T 2000-10-11 2001-10-05 Verfahren zur konstruktion von selbstassemlbierenden sonden und verfahren zu ihrer detektion Expired - Lifetime DE60123626T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000311151 2000-10-11
JP2000311151 2000-10-11
PCT/JP2001/008806 WO2002031192A1 (fr) 2000-10-11 2001-10-05 Procede de construction d"un autoassemblage de sondes et leur procede de detection

Publications (2)

Publication Number Publication Date
DE60123626D1 true DE60123626D1 (de) 2006-11-16
DE60123626T2 DE60123626T2 (de) 2007-01-18

Family

ID=18790969

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60123626T Expired - Lifetime DE60123626T2 (de) 2000-10-11 2001-10-05 Verfahren zur konstruktion von selbstassemlbierenden sonden und verfahren zu ihrer detektion

Country Status (8)

Country Link
US (1) US7122310B2 (de)
EP (1) EP1304386B1 (de)
JP (1) JP3912595B2 (de)
AT (1) ATE341647T1 (de)
AU (1) AU2001294199A1 (de)
DE (1) DE60123626T2 (de)
ES (1) ES2273892T3 (de)
WO (1) WO2002031192A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3310662B2 (ja) * 2000-03-31 2002-08-05 三光純薬株式会社 プローブポリマー作製用プローブ、プローブポリマーの作製方法及びその利用
US20030175689A1 (en) * 2000-08-30 2003-09-18 Mitsugu Usui Gene detecting method
WO2004072302A1 (ja) * 2003-02-14 2004-08-26 Eisai Co., Ltd. 発現遺伝子検出のためのシグナル増幅方法
CA2564838A1 (en) * 2004-04-28 2005-11-10 Eisai R&D Management Co., Ltd. Hybridization method
ATE462802T1 (de) * 2004-09-08 2010-04-15 Eisai R&D Man Co Ltd Verfahren zur bildung eines signalsondenpolymers
US7927804B2 (en) 2006-03-15 2011-04-19 Eisai & Managment Co., Ltd. Method of forming signal probe-polymer
US9315536B2 (en) * 2006-08-29 2016-04-19 Creatrone, Inc. Self-assembling oligonucleotides and methods
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
US8450058B2 (en) 2007-08-14 2013-05-28 Eisai R&D Management Co., Ltd. Method of detecting target substance
CN101835906A (zh) * 2007-10-24 2010-09-15 卫材R&D管理有限公司 核酸探针及探针聚合物的形成方法
WO2009057702A1 (ja) 2007-10-31 2009-05-07 Eisai R & D Management Co., Ltd. 標的物質検出用ポリマー及び標的物質の検出方法
JP2012070635A (ja) * 2009-01-29 2012-04-12 Eisai R & D Management Co Ltd 核酸の検出方法
WO2011125833A1 (ja) 2010-03-31 2011-10-13 学校法人 慶應義塾 生体分子相互作用解析ツールの構成とそれを用いた解析方法
WO2013085026A1 (ja) 2011-12-09 2013-06-13 エーザイ・アール・アンド・ディー・マネジメント株式会社 塩基変異の検出方法及び検出用キット
US20150299788A1 (en) 2012-05-15 2015-10-22 Eisai R&D Management Co., Ltd. Rna detection method and detection kit
EP3874067B1 (de) * 2018-10-30 2024-04-03 Institut National De La Recherche Scientifique Selbstassemblierende nukleinsäureoberflächen für biosensoranwendungen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5175270A (en) 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
CA2039517C (en) 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JP4221145B2 (ja) 1997-07-29 2009-02-12 ポリプローブ，インコーポレイテッド 最大の自己アセンブリを行う樹枝状核酸
JP3267576B2 (ja) * 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法

Also Published As

Publication number Publication date
EP1304386A4 (de) 2005-01-19
EP1304386B1 (de) 2006-10-04
ATE341647T1 (de) 2006-10-15
WO2002031192A1 (fr) 2002-04-18
US20030087262A1 (en) 2003-05-08
JPWO2002031192A1 (ja) 2004-02-19
EP1304386A1 (de) 2003-04-23
US7122310B2 (en) 2006-10-17
ES2273892T3 (es) 2007-05-16
DE60123626T2 (de) 2007-01-18
JP3912595B2 (ja) 2007-05-09
AU2001294199A1 (en) 2002-04-22

Similar Documents

Publication Publication Date Title
DE60123626D1 (de) Verfahren zur konstruktion von selbstassembierenden sonden und verfahren zu ihrer detektion
DE69233630T2 (de) Sätze von HIV-Sonden zur Verwendung in Sandwich-Hydridisierungsassays in Lösungsphase
US20010019835A1 (en) Gene amplifying method
EP1072679A3 (de) Methode zur Herstelung von Nucleinsäuremolekülen mit einer reduzierten Sekundärstruktur
CN101541978B (zh) 核酸探针以及检测疟原虫寄生虫的方法
DE602005020943D1 (de) Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
ATE466864T1 (de) Nichtaggregierende, nichtausschaltende oligomere, nukleotid-analoga enthaltend; verfahren zu deren herstellung und verwendung
WO1993016094A3 (en) Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
WO1999061665A2 (en) Solution hybridization of nucleic acids with antisense probes having modified backbones
EP1464710A3 (de) Eine Detektionsprobe und eine Reihe von Detektionsproben für Infektionserreger, ein Träger und eine genetische Uberprüfungsmethode
RU2004120769A (ru) Олигонуклеотид, контролирующий область гибридизации, и его применение
HU221237B1 (en) Solution phase nucleic acid sandwich assays having reduced background noise
CN106536735A (zh) 分析dna样品的探针集合和使用所述探针集合的方法
CN108138221A (zh) 用于检测核酸的超高灵敏度探针
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
KR20020033599A (ko) 프로우브 중합체 제작용 프로우브, 프로우브 중합체의제작방법 및 그 이용
WO1999016780A3 (en) Genetic sequences, diagnostic and/or quantification methods for the identification of staphylococci strains
DE19824900B4 (de) DNA-Nachweis über einen Strangreassoziationskomplex
EP1799860B1 (de) Dna-fingerabdrucktechnik unter verwendung eines branch-migration-tests
CA2516360A1 (en) Signal amplification method for detecting mutant gene
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
EP1026258A3 (de) Multiplex Genotypisierung von Populationen von Individuen
CA2389310A1 (en) Method of detecting gene
JP2003527866A5 (de)
EP2180062B1 (de) Verfahren zur erkennung einer zielsubstanz

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
R082 Change of representative

Ref document number: 1304386

Country of ref document: EP

Representative=s name: GRUENECKER, KINKELDEY, STOCKMAIR & SCHWANHAEUSSER,