WO2009057702A1 - 標的物質検出用ポリマー及び標的物質の検出方法 - Google Patents

標的物質検出用ポリマー及び標的物質の検出方法 Download PDF

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Publication number
WO2009057702A1
WO2009057702A1 PCT/JP2008/069778 JP2008069778W WO2009057702A1 WO 2009057702 A1 WO2009057702 A1 WO 2009057702A1 JP 2008069778 W JP2008069778 W JP 2008069778W WO 2009057702 A1 WO2009057702 A1 WO 2009057702A1
Authority
WO
WIPO (PCT)
Prior art keywords
target substance
polymer
detection
binding
detecting
Prior art date
Application number
PCT/JP2008/069778
Other languages
English (en)
French (fr)
Inventor
Motohito Kanashima
Chikako Hakii
Mitsugu Usui
Takashi Yamada
Hiroaki Honma
Original Assignee
Eisai R & D Management Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai R & D Management Co., Ltd. filed Critical Eisai R & D Management Co., Ltd.
Priority to JP2009539108A priority Critical patent/JPWO2009057702A1/ja
Priority to US12/734,362 priority patent/US20110171639A1/en
Priority to EP08844837A priority patent/EP2213751A1/en
Publication of WO2009057702A1 publication Critical patent/WO2009057702A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials

Abstract

　効率よく高感度且つ簡便に標的物質を検出することができる標的物質の検出方法、該方法に用いられる標的物質検出用ポリマー、及び該ポリマーの形成方法を提供する。標的物質の検出方法において、（Ａ）ポリマーを形成する複数種の核酸プローブ、及び標的物質に結合可能な領域と前記核酸プローブの少なくとも１つと結合可能な領域とを有する結合用プローブを溶液中で反応させることにより標的物質検出用ポリマーを形成する工程；（Ｂ）前記標的物質検出用ポリマー及び標的物質を結合させる工程；及び（Ｃ）前記標的物質が結合した標的物質検出用ポリマーを検出する工程を含むようにした。
PCT/JP2008/069778 2007-10-31 2008-10-30 標的物質検出用ポリマー及び標的物質の検出方法 WO2009057702A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009539108A JPWO2009057702A1 (ja) 2007-10-31 2008-10-30 標的物質検出用ポリマー及び標的物質の検出方法
US12/734,362 US20110171639A1 (en) 2007-10-31 2008-10-30 Polymer for detection of target substance, and method for detection of target substance
EP08844837A EP2213751A1 (en) 2007-10-31 2008-10-30 Polymer for detection of target substance, and method for detection of target substance

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-283672 2007-10-31
JP2007283672 2007-10-31
JP2008153974 2008-06-12
JP2008-153974 2008-06-12

Publications (1)

Publication Number Publication Date
WO2009057702A1 true WO2009057702A1 (ja) 2009-05-07

Family

ID=40591086

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/069778 WO2009057702A1 (ja) 2007-10-31 2008-10-30 標的物質検出用ポリマー及び標的物質の検出方法

Country Status (4)

Country Link
US (1) US20110171639A1 (ja)
EP (1) EP2213751A1 (ja)
JP (1) JPWO2009057702A1 (ja)
WO (1) WO2009057702A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013085026A1 (ja) * 2011-12-09 2013-06-13 エーザイ・アール・アンド・ディー・マネジメント株式会社 塩基変異の検出方法及び検出用キット
WO2013172305A1 (ja) * 2012-05-15 2013-11-21 エーザイ・アール・アンド・ディー・マネジメント株式会社 Ｒｎａの検出方法及び検出用キット
CN114539422A (zh) * 2020-11-25 2022-05-27 安升（上海）医药科技有限公司 核酸多聚化介导的多价蛋白药物和疫苗的构建方法及应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110352252A (zh) * 2016-09-22 2019-10-18 威廉马歇莱思大学 用于复杂序列捕获和分析的分子杂交探针

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020702A1 (en) 1991-05-24 1992-11-26 Ole Buchardt Peptide nucleic acids
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
WO2002031192A1 (fr) 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Procede de construction d"un autoassemblage de sondes et leur procede de detection
JP3310662B2 (ja) 2000-03-31 2002-08-05 三光純薬株式会社 プローブポリマー作製用プローブ、プローブポリマーの作製方法及びその利用
JP2002355081A (ja) 2001-03-21 2002-12-10 Sanko Junyaku Kk オリゴヌクレオチドによる自己集合体の作製方法及び遺伝子の検出方法
WO2003029441A1 (fr) 2001-09-28 2003-04-10 Sanko Junyaku Co., Ltd. Procede d'amplification des signaux de puces adn
WO2005106031A1 (ja) 2004-04-28 2005-11-10 Eisai R & D Management Co., Ltd. ハイブリダイゼーション方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09500378A (ja) * 1993-07-02 1997-01-14 リンクス セラピューティクス，インコーポレイティド 枝分れされそして複雑に結合された高分子構造体の集中的合成

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020702A1 (en) 1991-05-24 1992-11-26 Ole Buchardt Peptide nucleic acids
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
JP3310662B2 (ja) 2000-03-31 2002-08-05 三光純薬株式会社 プローブポリマー作製用プローブ、プローブポリマーの作製方法及びその利用
WO2002031192A1 (fr) 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Procede de construction d"un autoassemblage de sondes et leur procede de detection
JP2002355081A (ja) 2001-03-21 2002-12-10 Sanko Junyaku Kk オリゴヌクレオチドによる自己集合体の作製方法及び遺伝子の検出方法
WO2003029441A1 (fr) 2001-09-28 2003-04-10 Sanko Junyaku Co., Ltd. Procede d'amplification des signaux de puces adn
WO2005106031A1 (ja) 2004-04-28 2005-11-10 Eisai R & D Management Co., Ltd. ハイブリダイゼーション方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KOSHKIN AA ET AL., J. AM. CHEM. SOC., vol. 120, 1998, pages 13252 - 13253
KOSHKIN AA ET AL., TETRAHEDRON, vol. 54, 1998, pages 3607 - 3630
WAHLESTEDT C ET AL., PNAS, vol. 97, 2000, pages 5633 - 5638

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013085026A1 (ja) * 2011-12-09 2013-06-13 エーザイ・アール・アンド・ディー・マネジメント株式会社 塩基変異の検出方法及び検出用キット
WO2013172305A1 (ja) * 2012-05-15 2013-11-21 エーザイ・アール・アンド・ディー・マネジメント株式会社 Ｒｎａの検出方法及び検出用キット
CN114539422A (zh) * 2020-11-25 2022-05-27 安升（上海）医药科技有限公司 核酸多聚化介导的多价蛋白药物和疫苗的构建方法及应用
CN114539422B (zh) * 2020-11-25 2024-03-29 安升（上海）医药科技有限公司 核酸多聚化介导的多价蛋白药物和疫苗的构建方法及应用

Also Published As

Publication number Publication date
JPWO2009057702A1 (ja) 2011-03-10
US20110171639A1 (en) 2011-07-14
EP2213751A1 (en) 2010-08-04

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