DE60121372T2 - Methoden zur modifikation eukaryotischer zellen - Google Patents

Methoden zur modifikation eukaryotischer zellen Download PDF

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Publication number
DE60121372T2
DE60121372T2 DE60121372T DE60121372T DE60121372T2 DE 60121372 T2 DE60121372 T2 DE 60121372T2 DE 60121372 T DE60121372 T DE 60121372T DE 60121372 T DE60121372 T DE 60121372T DE 60121372 T2 DE60121372 T2 DE 60121372T2
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gene
cells
chromosomal locus
ltvec
dna
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DE60121372D1 (de
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N. Aris Tarrytown ECONOMIDES
J. Andrew Croton-on-Hudson MURPHY
M. David Yorktown Heights VALENZUELA
David New York FRENDEWEY
D. George Yorktown Heights YANCOPOULOS
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Regeneron Pharmaceuticals Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/105Murine

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60121372T 2000-10-31 2001-10-31 Methoden zur modifikation eukaryotischer zellen Expired - Lifetime DE60121372T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US24466500P 2000-10-31 2000-10-31
US244665P 2000-10-31
US732234 2000-12-07
US09/732,234 US6586251B2 (en) 2000-10-31 2000-12-07 Methods of modifying eukaryotic cells
PCT/US2001/045375 WO2002036789A2 (en) 2000-10-31 2001-10-31 Methods of modifying eukaryotic cells

Publications (2)

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DE60121372D1 DE60121372D1 (de) 2006-08-17
DE60121372T2 true DE60121372T2 (de) 2007-07-26

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EP (1) EP1399575B1 (cg-RX-API-DMAC7.html)
JP (7) JP5339566B2 (cg-RX-API-DMAC7.html)
KR (1) KR100833136B1 (cg-RX-API-DMAC7.html)
CN (1) CN100497642C (cg-RX-API-DMAC7.html)
AT (1) ATE332388T1 (cg-RX-API-DMAC7.html)
AU (2) AU2004802A (cg-RX-API-DMAC7.html)
BR (1) BRPI0115096B1 (cg-RX-API-DMAC7.html)
CA (1) CA2426398C (cg-RX-API-DMAC7.html)
CZ (1) CZ304856B6 (cg-RX-API-DMAC7.html)
DE (1) DE60121372T2 (cg-RX-API-DMAC7.html)
HU (1) HU227639B1 (cg-RX-API-DMAC7.html)
MX (1) MXPA03003797A (cg-RX-API-DMAC7.html)
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