DE60120733D1 - Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen - Google Patents

Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen

Info

Publication number
DE60120733D1
DE60120733D1 DE60120733T DE60120733T DE60120733D1 DE 60120733 D1 DE60120733 D1 DE 60120733D1 DE 60120733 T DE60120733 T DE 60120733T DE 60120733 T DE60120733 T DE 60120733T DE 60120733 D1 DE60120733 D1 DE 60120733D1
Authority
DE
Germany
Prior art keywords
banks
oligonucleotide
formation
libraries
mutation distribution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60120733T
Other languages
English (en)
Other versions
DE60120733T2 (de
Inventor
M Caldwell
Volker Schellenberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of DE60120733D1 publication Critical patent/DE60120733D1/de
Publication of DE60120733T2 publication Critical patent/DE60120733T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60120733T 2000-10-26 2001-10-12 Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen Expired - Lifetime DE60120733T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US699250 1996-08-19
US09/699,250 US6582914B1 (en) 2000-10-26 2000-10-26 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
PCT/US2001/032046 WO2002034762A2 (en) 2000-10-26 2001-10-12 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Publications (2)

Publication Number Publication Date
DE60120733D1 true DE60120733D1 (de) 2006-07-27
DE60120733T2 DE60120733T2 (de) 2007-06-14

Family

ID=24808514

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60120733T Expired - Lifetime DE60120733T2 (de) 2000-10-26 2001-10-12 Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen

Country Status (8)

Country Link
US (1) US6582914B1 (de)
EP (1) EP1328627B1 (de)
AT (1) ATE330008T1 (de)
AU (1) AU2002215346A1 (de)
CA (1) CA2426774A1 (de)
DE (1) DE60120733T2 (de)
DK (1) DK1328627T3 (de)
WO (1) WO2002034762A2 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340742A (en) * 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
EP1165235B1 (de) 1999-03-19 2011-09-28 Life Technologies Corporation Methode zum sichten von mutierten zellen
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP1608952B1 (de) 2002-12-20 2016-08-10 Life Technologies Corporation Assay vorrichtung und verfahren unter verwendung von mikrofluiden matrixstrukturen
US20050084868A1 (en) * 2003-10-16 2005-04-21 Wolfgang Aehle Generation of stabilized proteins by combinatorial consensus mutagenesis
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
CA2559171A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
US20050233336A1 (en) * 2004-04-19 2005-10-20 O'maille Paul E Compositions and methods for producing libraries with controlled compositions and screening probabilities
US7723077B2 (en) 2005-08-11 2010-05-25 Synthetic Genomics, Inc. In vitro recombination method
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
DK2032698T3 (da) * 2006-06-23 2012-09-03 Danisco Us Inc Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber
CA2665595A1 (en) * 2006-10-10 2008-04-17 Danisco Us, Inc., Genencor Division Glucoamylase variants with altered properties
US20100233780A1 (en) * 2007-06-06 2010-09-16 Wolfgang Aehle Methods for Improving Protein Properties
EP2481796B1 (de) 2007-10-09 2014-06-11 Danisco US Inc. Glucoamylasevarianten
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
RU2536255C2 (ru) 2007-11-01 2014-12-20 ДАНИСКО ЮЭс ИНК. Продуцирование термолизина и его вариантов и его использование в жидких детергентах
MX2010005461A (es) 2007-11-20 2010-06-02 Danisco Us Inc Variantes de glucoamilasa con propiedades alteradas.
SG191671A1 (en) 2007-12-13 2013-07-31 Danisco Us Inc Cultured cells that produce isoprene and methods for producing isoprene
EP3064599B1 (de) * 2008-02-15 2018-12-12 Synthetic Genomics, Inc. Verfahren zur in-vitro-verbindung und zur kombinatorischen anordnung von nukleinsäuremolekülen
AU2009240505B2 (en) 2008-04-23 2013-09-05 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
EP2130918A1 (de) 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
US20110256609A1 (en) 2008-06-06 2011-10-20 Basler Joshua R Compositions And Methods Comprising Variant Microbial Proteases
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
CN102209778B (zh) * 2008-11-11 2014-10-15 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
CA2743302A1 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Proteases comprising one or more combinable mutations
CA2759700A1 (en) 2009-04-23 2010-10-28 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
CN102712915B (zh) 2009-08-19 2014-12-10 丹尼斯科美国公司 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体
BR112012003787A2 (pt) 2009-08-19 2017-07-04 Danisco variantes de glicoamilase
CN102762222B (zh) 2009-12-09 2015-11-25 丹尼斯科美国公司 包含蛋白酶变体的组合物和方法
US20110281327A1 (en) 2010-04-15 2011-11-17 Danisco Us Inc. Compositions And Methods Comprising Variant Proteases
BR112012028466B1 (pt) 2010-05-06 2020-03-17 The Procter & Gamble Company Composição de produtos com variantes de protease e método para tratar e/ou limpar uma superfície
EP2633043A2 (de) 2010-10-27 2013-09-04 Danisco US Inc. Isoprensynthasevarianten zur verbesserten herstellung von isopren
EP2705145B1 (de) 2011-05-05 2020-06-17 The Procter and Gamble Company Zusammensetzungen und verfahren mit serinproteasevarianten
EP2705146B1 (de) 2011-05-05 2018-11-07 Danisco US Inc. Zusammensetzungen und verfahren mit serinproteasevarianten
CN104011067B (zh) 2011-12-22 2017-12-26 杜邦营养生物科学有限公司 具有葡糖淀粉酶活性的多肽及其制备方法
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
MX2015002099A (es) 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
MX2015005577A (es) 2012-11-05 2015-07-23 Danisco Inc Composiciones y metodos que comprenden variantes de proteasa termolisina.
WO2014124339A2 (en) 2013-02-07 2014-08-14 The Regents Of The University Of California Use of translational profiling to identify target molecules for therapeutic treatment
EP3044313B1 (de) 2013-09-12 2019-11-06 Danisco US Inc. Zusammensetzungen und verfahren mit protease varianten der lg12-klade
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
US9447445B2 (en) 2014-08-27 2016-09-20 New England Biolabs, Inc. Synthon formation
WO2016183509A1 (en) 2015-05-13 2016-11-17 Danisco Us Inc. AprL-CLADE PROTEASE VARIANTS AND USES THEREOF

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE93541T1 (de) 1985-07-03 1993-09-15 Genencor Int Hybride polypeptide und verfahren zu deren herstellung.
IL79881A (en) 1985-08-28 1995-12-08 Pieczenik George Populations of random coding series of oligonucleotides and peptides
US5556747A (en) 1990-07-09 1996-09-17 E. R. Squibb & Sons, Inc. Method for site-directed mutagenesis
WO1992002536A1 (en) * 1990-08-02 1992-02-20 The Regents Of The University Of Colorado Systematic polypeptide evolution by reverse translation
GB9027836D0 (en) 1990-12-21 1991-02-13 Unilever Plc Enzymes and enzymatic detergent compositions
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5521077A (en) 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
WO1997007205A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Method for preparing polypeptide variants
GB9521788D0 (en) 1995-10-24 1996-01-03 Univ Leeds DNA repair
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
JP2000512142A (ja) 1996-06-07 2000-09-19 マサチューセッツ インスティチュート オブ テクノロジー プログラムされた連続的変異誘発
JPH1066576A (ja) 1996-08-07 1998-03-10 Novo Nordisk As 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法
FR2753202A1 (fr) 1996-09-06 1998-03-13 Agronomique Inst Nat Rech Bacteries multiresistantes, procedes d'obtention et utilisation
US6046036A (en) 1996-10-25 2000-04-04 Advanced Research And Technology Institute DNA sequences encoding fusions of DNA repair proteins and uses thereof
CN1208456C (zh) 1996-12-20 2005-06-29 诺维信公司 体内重组
ATE468394T1 (de) 1997-03-18 2010-06-15 Novozymes As Vermischen von heterologen dns-sequenzen
EP0996718B1 (de) 1997-03-18 2008-12-24 Novozymes A/S Methode zur herstellung einer bibliothek mittels dna-shuffling
BR9808368A (pt) 1997-03-18 2000-05-23 Novo Nordisk As Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse.
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
CA2290074A1 (en) 1997-05-12 1998-11-19 Thermogen, Inc. Method for the stabilization of proteins and the thermostabilized alcohol dehydrogenases produced thereby
US6251604B1 (en) * 1999-08-13 2001-06-26 Genopsys, Inc. Random mutagenesis and amplification of nucleic acid

Also Published As

Publication number Publication date
WO2002034762A3 (en) 2003-03-13
US6582914B1 (en) 2003-06-24
DK1328627T3 (da) 2006-10-23
EP1328627B1 (de) 2006-06-14
DE60120733T2 (de) 2007-06-14
ATE330008T1 (de) 2006-07-15
CA2426774A1 (en) 2002-05-02
EP1328627A2 (de) 2003-07-23
AU2002215346A1 (en) 2002-05-06
WO2002034762A2 (en) 2002-05-02

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