AU2002215346A1 - Method for generating a library of oligonucleotides comprising a controlled distribution of mutations - Google Patents

Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Info

Publication number
AU2002215346A1
AU2002215346A1 AU2002215346A AU1534602A AU2002215346A1 AU 2002215346 A1 AU2002215346 A1 AU 2002215346A1 AU 2002215346 A AU2002215346 A AU 2002215346A AU 1534602 A AU1534602 A AU 1534602A AU 2002215346 A1 AU2002215346 A1 AU 2002215346A1
Authority
AU
Australia
Prior art keywords
oligonucleotides
mutations
library
generating
libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002215346A
Other languages
English (en)
Inventor
Robert M. Caldwell
Volker Schellenberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of AU2002215346A1 publication Critical patent/AU2002215346A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2002215346A 2000-10-26 2001-10-12 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations Abandoned AU2002215346A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/699,250 2000-10-26
US09/699,250 US6582914B1 (en) 2000-10-26 2000-10-26 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
PCT/US2001/032046 WO2002034762A2 (en) 2000-10-26 2001-10-12 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Publications (1)

Publication Number Publication Date
AU2002215346A1 true AU2002215346A1 (en) 2002-05-06

Family

ID=24808514

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002215346A Abandoned AU2002215346A1 (en) 2000-10-26 2001-10-12 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Country Status (8)

Country Link
US (1) US6582914B1 (de)
EP (1) EP1328627B1 (de)
AT (1) ATE330008T1 (de)
AU (1) AU2002215346A1 (de)
CA (1) CA2426774A1 (de)
DE (1) DE60120733T2 (de)
DK (1) DK1328627T3 (de)
WO (1) WO2002034762A2 (de)

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GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
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CA2665595A1 (en) * 2006-10-10 2008-04-17 Danisco Us, Inc., Genencor Division Glucoamylase variants with altered properties
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US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
ES2504983T3 (es) 2007-10-09 2014-10-09 Danisco Us Inc. Variantes de glucoamilasa
CN101868538B (zh) 2007-11-01 2013-07-10 丹尼斯科美国公司 嗜热菌蛋白酶及其变体的生产和在液体洗涤剂中的用途
HUE050328T2 (hu) 2007-11-20 2020-11-30 Danisco Us Inc Glükoamiláz változatok megváltoztatott tulajdonságokkal
RU2545699C2 (ru) 2007-12-13 2015-04-10 ДАНИСКО ЮЭс ИНК. Композиции и способы получения изопрена
EP3064599B1 (de) * 2008-02-15 2018-12-12 Synthetic Genomics, Inc. Verfahren zur in-vitro-verbindung und zur kombinatorischen anordnung von nukleinsäuremolekülen
AU2009240505B2 (en) * 2008-04-23 2013-09-05 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
EP2130918A1 (de) 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
CN104130993B (zh) 2008-06-06 2018-10-23 丹尼斯科美国公司 包括微生物蛋白酶变体的组合物和方法
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
WO2010056653A2 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Proteases comprising one or more combinable mutations
CA2743047A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising serine protease variants
US8518686B2 (en) * 2009-04-23 2013-08-27 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
CA2770607C (en) 2009-08-19 2019-02-26 Danisco A/S Variants of glucoamylase
US20120164695A1 (en) 2009-08-19 2012-06-28 Danisco Us Inc. Combinatorial variants of glucoamylase with improved specific activity and/or thermostability
MX338884B (es) 2009-12-09 2016-05-04 Danisco Us Inc Composiciones y metodos que comprenden variantes de proteasa.
CN102858970B (zh) 2010-04-15 2017-07-21 丹尼斯科美国公司 包含变体蛋白酶的组合物和方法
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Also Published As

Publication number Publication date
DK1328627T3 (da) 2006-10-23
CA2426774A1 (en) 2002-05-02
DE60120733T2 (de) 2007-06-14
EP1328627A2 (de) 2003-07-23
DE60120733D1 (de) 2006-07-27
WO2002034762A3 (en) 2003-03-13
EP1328627B1 (de) 2006-06-14
US6582914B1 (en) 2003-06-24
WO2002034762A2 (en) 2002-05-02
ATE330008T1 (de) 2006-07-15

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