DK1328627T3 - Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer - Google Patents

Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer

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Publication number
DK1328627T3
DK1328627T3 DK01983959T DK01983959T DK1328627T3 DK 1328627 T3 DK1328627 T3 DK 1328627T3 DK 01983959 T DK01983959 T DK 01983959T DK 01983959 T DK01983959 T DK 01983959T DK 1328627 T3 DK1328627 T3 DK 1328627T3
Authority
DK
Denmark
Prior art keywords
oligonucleotides
mutations
library
forming
libraries
Prior art date
Application number
DK01983959T
Other languages
English (en)
Inventor
Volker Schellenberger
Robert M Caldwell
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of DK1328627T3 publication Critical patent/DK1328627T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK01983959T 2000-10-26 2001-10-12 Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer DK1328627T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/699,250 US6582914B1 (en) 2000-10-26 2000-10-26 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Publications (1)

Publication Number Publication Date
DK1328627T3 true DK1328627T3 (da) 2006-10-23

Family

ID=24808514

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01983959T DK1328627T3 (da) 2000-10-26 2001-10-12 Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer

Country Status (8)

Country Link
US (1) US6582914B1 (da)
EP (1) EP1328627B1 (da)
AT (1) ATE330008T1 (da)
AU (1) AU2002215346A1 (da)
CA (1) CA2426774A1 (da)
DE (1) DE60120733T2 (da)
DK (1) DK1328627T3 (da)
WO (1) WO2002034762A2 (da)

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CN101784662B (zh) * 2007-06-06 2014-11-05 丹尼斯科美国公司 用于改善蛋白质特性的方法
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
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CA2705941C (en) 2007-11-20 2018-05-22 Danisco Us Inc. Glucoamylase variants with altered properties
CN102027124B (zh) 2007-12-13 2015-11-25 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
SG10201800778QA (en) * 2008-02-15 2018-02-27 Synthetic Genomics Inc Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
EP2279251A2 (en) * 2008-04-23 2011-02-02 Danisco US Inc. Isoprene synthase variants for improved microbial production of isoprene
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AU2009256169A1 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Compositions and methods comprising variant microbial proteases
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
EP2650354A3 (en) 2008-11-11 2014-02-12 The Procter and Gamble Company Bacillus subtilisin comprising one or more combinable mutations
KR20110091671A (ko) 2008-11-11 2011-08-12 다니스코 유에스 인크. 세린 프로테아제 변이체를 포함하는 조성물 및 방법
US8518686B2 (en) * 2009-04-23 2013-08-27 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
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Also Published As

Publication number Publication date
DE60120733D1 (de) 2006-07-27
US6582914B1 (en) 2003-06-24
EP1328627B1 (en) 2006-06-14
ATE330008T1 (de) 2006-07-15
WO2002034762A3 (en) 2003-03-13
AU2002215346A1 (en) 2002-05-06
EP1328627A2 (en) 2003-07-23
WO2002034762A2 (en) 2002-05-02
CA2426774A1 (en) 2002-05-02
DE60120733T2 (de) 2007-06-14

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