ATE330008T1 - Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen - Google Patents

Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen

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Publication number
ATE330008T1
ATE330008T1 AT01983959T AT01983959T ATE330008T1 AT E330008 T1 ATE330008 T1 AT E330008T1 AT 01983959 T AT01983959 T AT 01983959T AT 01983959 T AT01983959 T AT 01983959T AT E330008 T1 ATE330008 T1 AT E330008T1
Authority
AT
Austria
Prior art keywords
banks
forming oligonucleotide
libraries
mutation distribution
controlled mutation
Prior art date
Application number
AT01983959T
Other languages
English (en)
Inventor
Robert M Caldwell
Volker Schellenberger
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE330008T1 publication Critical patent/ATE330008T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
AT01983959T 2000-10-26 2001-10-12 Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen ATE330008T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/699,250 US6582914B1 (en) 2000-10-26 2000-10-26 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Publications (1)

Publication Number Publication Date
ATE330008T1 true ATE330008T1 (de) 2006-07-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AT01983959T ATE330008T1 (de) 2000-10-26 2001-10-12 Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen

Country Status (8)

Country Link
US (1) US6582914B1 (de)
EP (1) EP1328627B1 (de)
AT (1) ATE330008T1 (de)
AU (1) AU2002215346A1 (de)
CA (1) CA2426774A1 (de)
DE (1) DE60120733T2 (de)
DK (1) DK1328627T3 (de)
WO (1) WO2002034762A2 (de)

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Also Published As

Publication number Publication date
DE60120733T2 (de) 2007-06-14
US6582914B1 (en) 2003-06-24
EP1328627A2 (de) 2003-07-23
WO2002034762A2 (en) 2002-05-02
AU2002215346A1 (en) 2002-05-06
WO2002034762A3 (en) 2003-03-13
CA2426774A1 (en) 2002-05-02
EP1328627B1 (de) 2006-06-14
DE60120733D1 (de) 2006-07-27
DK1328627T3 (da) 2006-10-23

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