DK1328627T3 - Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer - Google Patents

Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer

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Publication number
DK1328627T3
DK1328627T3 DK01983959T DK01983959T DK1328627T3 DK 1328627 T3 DK1328627 T3 DK 1328627T3 DK 01983959 T DK01983959 T DK 01983959T DK 01983959 T DK01983959 T DK 01983959T DK 1328627 T3 DK1328627 T3 DK 1328627T3
Authority
DK
Denmark
Prior art keywords
oligonucleotides
mutations
library
forming
libraries
Prior art date
Application number
DK01983959T
Other languages
Danish (da)
English (en)
Inventor
Volker Schellenberger
Robert M Caldwell
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of DK1328627T3 publication Critical patent/DK1328627T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK01983959T 2000-10-26 2001-10-12 Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer DK1328627T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/699,250 US6582914B1 (en) 2000-10-26 2000-10-26 Method for generating a library of oligonucleotides comprising a controlled distribution of mutations

Publications (1)

Publication Number Publication Date
DK1328627T3 true DK1328627T3 (da) 2006-10-23

Family

ID=24808514

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01983959T DK1328627T3 (da) 2000-10-26 2001-10-12 Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer

Country Status (8)

Country Link
US (1) US6582914B1 (de)
EP (1) EP1328627B1 (de)
AT (1) ATE330008T1 (de)
AU (1) AU2002215346A1 (de)
CA (1) CA2426774A1 (de)
DE (1) DE60120733T2 (de)
DK (1) DK1328627T3 (de)
WO (1) WO2002034762A2 (de)

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US20050084868A1 (en) * 2003-10-16 2005-04-21 Wolfgang Aehle Generation of stabilized proteins by combinatorial consensus mutagenesis
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
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US20050233336A1 (en) * 2004-04-19 2005-10-20 O'maille Paul E Compositions and methods for producing libraries with controlled compositions and screening probabilities
CA2618699C (en) * 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
WO2008002472A2 (en) * 2006-06-23 2008-01-03 Danisco Us Inc., Genencor Division Systematic evaluation of sequence and activity relationships using site evaluation libraries for engineering multiple properties
US9447397B2 (en) 2006-10-10 2016-09-20 Danisco Us Inc. Glucoamylase variants with altered properties
US20100233780A1 (en) * 2007-06-06 2010-09-16 Wolfgang Aehle Methods for Improving Protein Properties
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
JP5463292B2 (ja) 2007-10-09 2014-04-09 ダニスコ・ユーエス・インク グルコアミラーゼ変異体
CA2704311C (en) 2007-11-01 2018-02-13 Danisco Us Inc. Production of thermolysin and variants thereof, and use in liquid detergents
HUE050328T2 (hu) 2007-11-20 2020-11-30 Danisco Us Inc Glükoamiláz változatok megváltoztatott tulajdonságokkal
SG191671A1 (en) 2007-12-13 2013-07-31 Danisco Us Inc Cultured cells that produce isoprene and methods for producing isoprene
US8968999B2 (en) * 2008-02-15 2015-03-03 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
SG192545A1 (en) 2008-04-23 2013-08-30 Danisco Us Inc Isoprene synthase variants for improved microbial production of isoprene
EP2130918A1 (de) 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
EP2578680B1 (de) 2008-06-06 2016-04-27 Danisco US Inc. Zusammensetzungen und Verfahren mit mikrobiellen Proteasevarianten
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
WO2010056653A2 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Proteases comprising one or more combinable mutations
WO2010056640A2 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising serine protease variants
SG10201501879PA (en) 2009-04-23 2015-05-28 Danisco Us Inc Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP5827227B2 (ja) 2009-08-19 2015-12-02 デュポン ニュートリション バイオサイエンシーズ エーピーエスＤｕＰｏｎｔ Ｎｕｔｒｉｔｉｏｎ Ｂｉｏｓｃｉｅｎｃｅｓ ＡｐＳ グルコアミラーゼの変異体
WO2011022465A1 (en) 2009-08-19 2011-02-24 Danisco Us Inc. Combinatorial variants of glucoamylase with improved specific activity and/or thermostability
AR079338A1 (es) 2009-12-09 2012-01-18 Danisco Us Inc Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes
PL2558573T3 (pl) 2010-04-15 2017-08-31 Danisco Us Inc. Kompozycje i sposoby obejmujące warianty proteazy
US20130260438A1 (en) 2010-05-06 2013-10-03 Danisco Us Inc. Compositions and methods comprising serine protease variants (as amended)
JP2014502148A (ja) 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体
BR112013027963A2 (pt) 2011-05-05 2016-11-29 Danisco Us Inc "variante de subtilisina com atividade proteolítica, ácido nucleico, vetor de expressão, célula hospedeira, composição e método de limpeza".
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US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
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Also Published As

Publication number Publication date
DE60120733T2 (de) 2007-06-14
EP1328627B1 (de) 2006-06-14
AU2002215346A1 (en) 2002-05-06
DE60120733D1 (de) 2006-07-27
EP1328627A2 (de) 2003-07-23
WO2002034762A3 (en) 2003-03-13
US6582914B1 (en) 2003-06-24
ATE330008T1 (de) 2006-07-15
WO2002034762A2 (en) 2002-05-02
CA2426774A1 (en) 2002-05-02

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