ATE464382T1 - Mutageneseverfahren - Google Patents

Mutageneseverfahren

Info

Publication number
ATE464382T1
ATE464382T1 AT06701755T AT06701755T ATE464382T1 AT E464382 T1 ATE464382 T1 AT E464382T1 AT 06701755 T AT06701755 T AT 06701755T AT 06701755 T AT06701755 T AT 06701755T AT E464382 T1 ATE464382 T1 AT E464382T1
Authority
AT
Austria
Prior art keywords
mutagenic primer
nucleic acid
mutations
synthesising
stranded
Prior art date
Application number
AT06701755T
Other languages
English (en)
Inventor
Ralph Minter
Steven Lane
Robert Holgate
Maria Groves
Original Assignee
Medimmune Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0500417.1A external-priority patent/GB0500417D0/en
Application filed by Medimmune Ltd filed Critical Medimmune Ltd
Application granted granted Critical
Publication of ATE464382T1 publication Critical patent/ATE464382T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT06701755T 2005-01-10 2006-01-09 Mutageneseverfahren ATE464382T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US64272905P 2005-01-10 2005-01-10
GBGB0500417.1A GB0500417D0 (en) 2005-01-10 2005-01-10 Method of mutagenesis
PCT/GB2006/000059 WO2006072801A1 (en) 2005-01-10 2006-01-09 Method of mutagenesis

Publications (1)

Publication Number Publication Date
ATE464382T1 true ATE464382T1 (de) 2010-04-15

Family

ID=35911570

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06701755T ATE464382T1 (de) 2005-01-10 2006-01-09 Mutageneseverfahren

Country Status (13)

Country Link
EP (1) EP1844144B1 (de)
JP (2) JP5789073B2 (de)
AT (1) ATE464382T1 (de)
AU (1) AU2006204396B2 (de)
CA (1) CA2593417C (de)
DE (1) DE602006013596D1 (de)
DK (1) DK1844144T3 (de)
ES (1) ES2343648T3 (de)
GB (1) GB2421950B (de)
PL (1) PL1844144T3 (de)
PT (1) PT1844144E (de)
SI (1) SI1844144T1 (de)
WO (1) WO2006072801A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008339576B2 (en) 2007-12-21 2014-05-22 Medimmune Limited Binding members for interleukin-4 receptor alpha (IL-4Ralpha)
US8092804B2 (en) 2007-12-21 2012-01-10 Medimmune Limited Binding members for interleukin-4 receptor alpha (IL-4Rα)-173
US8980273B1 (en) 2014-07-15 2015-03-17 Kymab Limited Method of treating atopic dermatitis or asthma using antibody to IL4RA
US8986691B1 (en) 2014-07-15 2015-03-24 Kymab Limited Method of treating atopic dermatitis or asthma using antibody to IL4RA
WO2020180700A1 (en) * 2019-03-01 2020-09-10 Fred Hutchinson Cancer Research Center Protein residue mapping using a combination of deep mutational scanning and phage display high throughput sequencing

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
DE4122599C2 (de) * 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
ATE239089T1 (de) * 1991-12-24 2003-05-15 Harvard College Gezielte punkt-mutagenese von dna
JP3720353B2 (ja) 1992-12-04 2005-11-24 メディカル リサーチ カウンシル 多価および多重特異性の結合タンパク質、それらの製造および使用
WO2000058517A1 (en) * 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5789166A (en) * 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
US6620597B1 (en) * 1998-01-09 2003-09-16 University Of Utah Research Foundation Method for in vitro amplification of circular DNA
JP2002534966A (ja) * 1999-01-19 2002-10-22 マキシジェン, インコーポレイテッド オリゴヌクレオチド媒介核酸組換え
JP3859947B2 (ja) * 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction

Also Published As

Publication number Publication date
WO2006072801A1 (en) 2006-07-13
PL1844144T3 (pl) 2010-09-30
DE602006013596D1 (de) 2010-05-27
SI1844144T1 (sl) 2010-07-30
GB2421950B (en) 2007-03-21
ES2343648T3 (es) 2010-08-05
JP2015171369A (ja) 2015-10-01
GB2421950A (en) 2006-07-12
DK1844144T3 (da) 2010-07-19
GB0600331D0 (en) 2006-02-15
JP2008526223A (ja) 2008-07-24
CA2593417C (en) 2014-10-28
EP1844144B1 (de) 2010-04-14
AU2006204396A1 (en) 2006-07-13
JP5789073B2 (ja) 2015-10-07
AU2006204396B2 (en) 2011-08-11
CA2593417A1 (en) 2006-07-13
EP1844144A1 (de) 2007-10-17
PT1844144E (pt) 2010-06-11

Similar Documents

Publication Publication Date Title
PT2089343E (pt) Química click para a produção de moléculas repórter
WO2009123762A3 (en) Method of screening single cells for the production of biologically active agents
WO2015108993A8 (en) Mutagenesis methods
SI2143792T1 (sl) Enoverižna ciklična RNA in postopek za njeno pripravo
SG10201408300PA (en) Processing of amplified dna fragments for sequencing
MX2007006549A (es) Procedimiento para preparar derivados condensados de piperazin-2-ona, asi como productos intermedios del procedimiento.
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
WO2008012343A3 (en) Macrolide synthesis process
WO2006024387A3 (de) Biphenylthiazolcarboxamide
WO2012104399A3 (en) Device and method for the generation of molecular microarrays
ATE464382T1 (de) Mutageneseverfahren
ATE468349T1 (de) GROßTECHNISCHES VERFAHREN ZUR HERSTELLUNG VON 17- HYDROXY-6BETA, 7BETA, 15BETA,16BETA-BISMETHYLEN-3-OXO-17ALPHA-PREGN-4 EN-21-CARBONSÄURE-GAMMA- LACTON UND SCHLÜSSELZWISCHENPRODUKTE FÜR DIESES VERFAHREN
FR2804971B1 (fr) Adn codant une acetohydroxyacide synthase, bacterie le contenant et procede de production de l-valine par culture de cette bacterie
WO2006091731A3 (en) Processes for the preparation of linezolid intermediate
WO2016176654A3 (en) COMPOSITIONS AND METHODS FOR CONSTRUCTING STRAND SPECIFIC cDNA LIBRARIES
EP1806401A4 (de) Für ein neuartiges enzymmit d-serin-synthaseaktivität codierende dna, verfahren zur herstellung desenzyms und verfahren zur herstellung von d-serin unter verwendung davon
TW200738650A (en) Process for synthesis of aryloxy diaminopyrimidines
WO2003100072A3 (de) Verfahren zur fermentativen herstellung schwefelhaltiger feinchemikalien
ATE530590T1 (de) Verfahren zur herstellung von polyesterolen
DE60123334D1 (de) Methode für die Produktion von Nukleotiden durch Fermentierung
MX2021005028A (es) Ensamble determinista multiplexado de genotecas de adn.
WO2006103111A3 (en) Improved bisulfite conversion of dna
WO2006055836A3 (en) In vivo alteration of cellular dna
ATE486999T1 (de) Vorrichtung und verfahren zur herstellung von cellulosebasierten produkten
WO2009044782A1 (ja) 一本鎖又は二本鎖dnaの合成方法および合成キット

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1844144

Country of ref document: EP

REN Ceased due to non-payment of the annual fee