DE60028534D1 - Binärsonde, klammerzusammensetzung und verfahren zum zielhybridisierungsnachweis - Google Patents
Binärsonde, klammerzusammensetzung und verfahren zum zielhybridisierungsnachweisInfo
- Publication number
- DE60028534D1 DE60028534D1 DE60028534T DE60028534T DE60028534D1 DE 60028534 D1 DE60028534 D1 DE 60028534D1 DE 60028534 T DE60028534 T DE 60028534T DE 60028534 T DE60028534 T DE 60028534T DE 60028534 D1 DE60028534 D1 DE 60028534D1
- Authority
- DE
- Germany
- Prior art keywords
- detection
- probe
- target hybridization
- hybridization procedure
- procedure method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/232,000 US6432642B1 (en) | 1999-01-15 | 1999-01-15 | Binary probe and clamp composition and methods for a target hybridization detection |
US232000 | 1999-01-15 | ||
PCT/US2000/000972 WO2000041549A2 (en) | 1999-01-15 | 2000-01-14 | Binary probe and clamp composition and methods for target hybridization detection |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60028534D1 true DE60028534D1 (de) | 2006-07-20 |
DE60028534T2 DE60028534T2 (de) | 2007-06-06 |
Family
ID=22871476
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60028534T Expired - Lifetime DE60028534T2 (de) | 1999-01-15 | 2000-01-14 | Binärsonde, klammerzusammensetzung und verfahren zum zielhybridisierungsnachweis |
Country Status (8)
Country | Link |
---|---|
US (4) | US6432642B1 (de) |
EP (1) | EP1141387B1 (de) |
JP (3) | JP4644370B2 (de) |
AT (1) | ATE329056T1 (de) |
AU (1) | AU771650B2 (de) |
CA (1) | CA2360543C (de) |
DE (1) | DE60028534T2 (de) |
WO (1) | WO2000041549A2 (de) |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6432642B1 (en) * | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
US6395486B1 (en) | 1999-03-15 | 2002-05-28 | Applera Corporation | Probe/mobility modifier complexes for multiplexnucleic acid detection |
US7118860B2 (en) * | 1999-10-29 | 2006-10-10 | Stratagene California | Methods for detection of a target nucleic acid by capture |
US6977161B2 (en) | 2000-10-14 | 2005-12-20 | Eragen Biosciences, Inc. | Solid support assay systems and methods utilizing non-standard bases |
WO2001090417A2 (en) * | 2000-05-19 | 2001-11-29 | Eragen Biosciences, Inc. | Materials and methods for detection of nucleic acids |
US20030082549A1 (en) * | 2000-08-30 | 2003-05-01 | Xiangjun Liu | Method for determining alleles |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
WO2003100095A1 (fr) * | 2002-05-08 | 2003-12-04 | Arkray, Inc. | Procede de detection d'un acide nucleique cible |
WO2005001129A2 (en) * | 2003-06-06 | 2005-01-06 | Applera Corporation | Mobility cassettes |
GB0320235D0 (en) * | 2003-08-29 | 2003-10-01 | Molecular Light Tech Res Ltd | Estimation of activity or inhibition of processes involved in nucleic acid modification using chemiluminescence quenching |
US7575863B2 (en) | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
WO2006112818A2 (en) * | 2005-04-14 | 2006-10-26 | Applera Corporation | 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes |
US7767834B2 (en) * | 2004-08-13 | 2010-08-03 | Elitech Holding B.V. | Phosphonylated fluorescent dyes and conjugates |
WO2006020947A2 (en) | 2004-08-13 | 2006-02-23 | Epoch Biosciences, Inc. | Phosphonate fluorescent dyes and conjugates |
EP1791982B1 (de) * | 2004-09-21 | 2011-01-05 | Life Technologies Corporation | Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) |
US7588921B1 (en) * | 2004-09-22 | 2009-09-15 | University Of Central Florida Research Foundation, Inc. | Messenger RNA profiling: body fluid identification using multiplex real time-polymerase chain reaction (q-PCR) |
US7700287B2 (en) | 2005-01-28 | 2010-04-20 | Life Technologies Corporation | Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template |
GB0510979D0 (en) * | 2005-05-28 | 2005-07-06 | Kbiosciences Ltd | Detection system for PCR assay |
ES2494922T3 (es) * | 2005-09-01 | 2014-09-16 | Ausdiagnostics Pty Ltd. | Métodos para la amplificación, cuantificación e identificación de ácidos nucleicos |
FR2900158A1 (fr) * | 2006-04-25 | 2007-10-26 | Biomerieux Sa | Nouvelle sonde de detection agissant par reconnaissance moleculaire |
US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
ATE538216T1 (de) * | 2006-07-31 | 2012-01-15 | Wanli Bi | Nukleinsäureverstärkung mithilfe eines reversibel modifizierten oligonukleotids |
US9045522B2 (en) | 2006-07-31 | 2015-06-02 | Wanli Bi | Nucleic acid amplification using a reversibly modified oligonucleotide |
WO2008021446A2 (en) * | 2006-08-15 | 2008-02-21 | Genetag Technology, Inc. | Probe-antiprobe compositions and methods for dna or rna detection |
FR2906532B1 (fr) * | 2006-09-28 | 2008-12-12 | Biomerieux Sa | Nouvel oligonucleotide marque |
US9938641B2 (en) * | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
EP1995327A1 (de) * | 2007-05-21 | 2008-11-26 | Humboldt Universität zu Berlin | Sonde zur Erkennung einer bestimmten Nukleinsäuresequenz |
CN102007222A (zh) * | 2007-08-13 | 2011-04-06 | 3M创新有限公司 | 用于检测耐药微生物的方法 |
LT2660336T (lt) * | 2008-06-11 | 2017-10-10 | Orion Pharma (Uk) Limited | Izoterminė nukleorūgšties amplifkacija |
FR2933410B1 (fr) * | 2008-07-04 | 2010-08-20 | Biomerieux Sa | Nouvelle sonde de detection |
US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
US9914963B2 (en) | 2009-09-28 | 2018-03-13 | Igor Kutyavin | Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes |
WO2012106668A2 (en) * | 2011-02-03 | 2012-08-09 | Fluidigm Corporation | Multifunctional probe-primers |
WO2013009070A2 (ko) * | 2011-07-12 | 2013-01-17 | 주식회사 파나진 | 평행 결합 구조의 이중 혼성화 pna 프로브 시스템 |
US9133509B2 (en) | 2012-03-22 | 2015-09-15 | Lgc Genomics Limited | Polymerase chain reaction detection system |
EP2989212B1 (de) | 2013-04-25 | 2018-07-18 | Orion Diagnostica Oy | Dna-amplifizierung auf stranginvasionsbasis |
EP3726213A1 (de) | 2013-08-19 | 2020-10-21 | Singular Bio Inc. | Assays zur einzelmoleküldetektion und verwendung davon |
GB201410022D0 (en) | 2014-06-05 | 2014-07-16 | Orion Diagnostica Oy | Method |
CN106715768B (zh) | 2014-07-30 | 2020-06-16 | 哈佛学院院长及董事 | 用于测定核酸的系统和方法 |
US11739371B2 (en) | 2015-02-18 | 2023-08-29 | Invitae Corporation | Arrays for single molecule detection and use thereof |
US11788123B2 (en) | 2017-05-26 | 2023-10-17 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
KR101899371B1 (ko) * | 2017-07-25 | 2018-10-29 | (주)엔바이오텍 | 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법 |
KR102262260B1 (ko) * | 2018-02-08 | 2021-06-09 | 주식회사 시선테라퓨틱스 | 엔도좀 탈출능을 갖는 펩티드 핵산 복합체 및 이의 용도 |
CN110542674B (zh) * | 2019-09-19 | 2021-10-22 | 济南大学 | 一种检测谷胱甘肽的生物传感器及其制备方法 |
WO2022261030A1 (en) * | 2021-06-07 | 2022-12-15 | Neubase Therapeutics, Inc. | Oligonucleotide analogue modulators of oncogenes |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
GB8405437D0 (en) | 1984-03-01 | 1984-04-04 | Amersham Int Plc | Detecting polynucleotide sequences |
ATE65092T1 (de) | 1985-05-31 | 1991-07-15 | Amoco Corp | Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. |
US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US4931223A (en) | 1986-07-24 | 1990-06-05 | Tropix, Inc. | Methods of using chemiluminescent 1,2-dioxetanes |
AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
US5656731A (en) | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
US5231191A (en) | 1987-12-24 | 1993-07-27 | Applied Biosystems, Inc. | Rhodamine phosphoramidite compounds |
US5175273A (en) * | 1988-07-01 | 1992-12-29 | Genentech, Inc. | Nucleic acid intercalating agents |
US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5627032A (en) * | 1990-12-24 | 1997-05-06 | Ulanovsky; Levy | Composite primers for nucleic acids |
US5539082A (en) * | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
US5594121A (en) | 1991-11-07 | 1997-01-14 | Gilead Sciences, Inc. | Enhanced triple-helix and double-helix formation with oligomers containing modified purines |
JP3739785B2 (ja) | 1991-11-26 | 2006-01-25 | アイシス ファーマシューティカルズ,インコーポレイティド | 修飾されたピリミジンを含有するオリゴマーを使用する増強された三重らせんおよび二重らせんの成形 |
US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
FR2710075B1 (fr) * | 1993-09-15 | 1995-10-27 | Bio Merieux | Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal. |
US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
US5602004A (en) * | 1994-07-20 | 1997-02-11 | Novo Nordisk Biotech, Inc. | Thermophilic fungal expression system |
US5552471A (en) | 1994-08-17 | 1996-09-03 | The Perkin-Elmer Corporation | Solid support reagents for the synthesis of 3'-Nitrogen containing polynucleotides |
US5801155A (en) | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
WO1997019193A2 (en) * | 1995-11-21 | 1997-05-29 | Yale University | Unimolecular segment amplification and detection |
US5736626A (en) | 1996-01-29 | 1998-04-07 | The Perkin-Elmer Corporation | Solid support reagents for the direct synthesis of 3'-labeled polynucleotides |
SE506700C2 (sv) | 1996-05-31 | 1998-02-02 | Mikael Kubista | Sond och förfaranden för analys av nukleinsyra |
CA2218440A1 (en) | 1996-12-21 | 1998-06-21 | Boehringer Mannheim Gmbh | Method of analysis using signal amplification |
EP0857791B1 (de) | 1996-12-21 | 2004-03-31 | PNA Diagnostics A/S | Analyseverfahren basierend auf Signalverstärkung |
US6287772B1 (en) * | 1998-04-29 | 2001-09-11 | Boston Probes, Inc. | Methods, kits and compositions for detecting and quantitating target sequences |
US6435642B1 (en) * | 1998-11-17 | 2002-08-20 | Pitney Bowes Inc. | Apparatus and method for real-time measurement of digital print quality |
US6432642B1 (en) * | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
-
1999
- 1999-01-15 US US09/232,000 patent/US6432642B1/en not_active Expired - Lifetime
-
2000
- 2000-01-14 DE DE60028534T patent/DE60028534T2/de not_active Expired - Lifetime
- 2000-01-14 WO PCT/US2000/000972 patent/WO2000041549A2/en active IP Right Grant
- 2000-01-14 AT AT00906924T patent/ATE329056T1/de not_active IP Right Cessation
- 2000-01-14 JP JP2000593170A patent/JP4644370B2/ja not_active Expired - Lifetime
- 2000-01-14 CA CA002360543A patent/CA2360543C/en not_active Expired - Fee Related
- 2000-01-14 EP EP00906924A patent/EP1141387B1/de not_active Expired - Lifetime
- 2000-01-14 AU AU28504/00A patent/AU771650B2/en not_active Ceased
-
2002
- 2002-03-28 US US10/112,677 patent/US7057025B2/en not_active Expired - Lifetime
-
2005
- 2005-02-23 JP JP2005047966A patent/JP2005160489A/ja not_active Withdrawn
-
2006
- 2006-06-05 US US11/422,211 patent/US20070026429A1/en not_active Abandoned
-
2008
- 2008-07-29 JP JP2008195452A patent/JP2009017882A/ja active Pending
-
2009
- 2009-08-10 US US12/538,823 patent/US8067165B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US8067165B2 (en) | 2011-11-29 |
ATE329056T1 (de) | 2006-06-15 |
EP1141387A2 (de) | 2001-10-10 |
CA2360543C (en) | 2009-03-31 |
WO2000041549A2 (en) | 2000-07-20 |
US20100291557A1 (en) | 2010-11-18 |
US20060035217A1 (en) | 2006-02-16 |
DE60028534T2 (de) | 2007-06-06 |
US6432642B1 (en) | 2002-08-13 |
JP4644370B2 (ja) | 2011-03-02 |
JP2002534100A (ja) | 2002-10-15 |
AU2850400A (en) | 2000-08-01 |
EP1141387B1 (de) | 2006-06-07 |
WO2000041549A3 (en) | 2000-11-30 |
JP2005160489A (ja) | 2005-06-23 |
US7057025B2 (en) | 2006-06-06 |
JP2009017882A (ja) | 2009-01-29 |
US20070026429A1 (en) | 2007-02-01 |
AU771650B2 (en) | 2004-04-01 |
CA2360543A1 (en) | 2000-07-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US |
|
8380 | Miscellaneous part iii |
Free format text: PFANDRECHT |
|
8380 | Miscellaneous part iii |
Free format text: PFANDRECHT AUFGEHOBEN |