WO2001081620A3 - Verfahren zur hochparallelen analyse von polymorphismen - Google Patents

Verfahren zur hochparallelen analyse von polymorphismen Download PDF

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Publication number
WO2001081620A3
WO2001081620A3 PCT/DE2001/001607 DE0101607W WO0181620A3 WO 2001081620 A3 WO2001081620 A3 WO 2001081620A3 DE 0101607 W DE0101607 W DE 0101607W WO 0181620 A3 WO0181620 A3 WO 0181620A3
Authority
WO
WIPO (PCT)
Prior art keywords
probes
allele
analyzed
polymorphisms
highly parallel
Prior art date
Application number
PCT/DE2001/001607
Other languages
English (en)
French (fr)
Other versions
WO2001081620A2 (de
Inventor
Kurt Berlin
Ivo Glynne Gut
Original Assignee
Epigenomics Ag
Kurt Berlin
Ivo Glynne Gut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Kurt Berlin, Ivo Glynne Gut filed Critical Epigenomics Ag
Priority to AU2001265759A priority Critical patent/AU2001265759A1/en
Priority to EP01942981A priority patent/EP1309728A2/de
Priority to DE10191554T priority patent/DE10191554D2/de
Publication of WO2001081620A2 publication Critical patent/WO2001081620A2/de
Publication of WO2001081620A3 publication Critical patent/WO2001081620A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Beschrieben wird ein Verfahren zur hochparallelen Charakterisierung von Polymorphismen, insbesondere SNPs, das auch für die gleichzeitige oder separate Detektion von DNA-Methylierung eingesetzt werden kann. Zuerst bindet man einen Satz von Sonden, der mit mindestens einer für die jeweilige Sonde charakteristischen nachweisbaren Markierung versehen ist, an eine adressierte Oberfläche, wobei die erzeugte Bindung der Sonden an die Oberfläche photochemisch, chemisch oder enzymatisch wieder spaltbar ist. Anschliessend hybridisiert man eine zu untersuchende Nukleinsäure an diese Sonden, verändert die Sonden in einer allelspezifischen enzymatischen Reaktion und entfernt einen Teil der Sonden, der für die Analyse der allelspezifischen Reaktion bedeutungslos ist. Zuletzt analysiert man die allelespezifischen Produkte anhand der nachweisbaren Markierungen und führt eine Bestimmung der vorhandenen Allele in der abgefragten Nukleinsäureprobe durch.
PCT/DE2001/001607 2000-04-25 2001-04-25 Verfahren zur hochparallelen analyse von polymorphismen WO2001081620A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2001265759A AU2001265759A1 (en) 2000-04-25 2001-04-25 Method for the highly parallel analysis of polymorphisms
EP01942981A EP1309728A2 (de) 2000-04-25 2001-04-25 Verfahren zur hochparallelen analyse von polymorphismen
DE10191554T DE10191554D2 (de) 2000-04-25 2001-04-25 Verfahren zur hochparallelen Analyse von Polymorphismen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10021204.2 2000-04-25
DE10021204A DE10021204A1 (de) 2000-04-25 2000-04-25 Verfahren zur hochparallelen Analyse von Polymorphismen

Publications (2)

Publication Number Publication Date
WO2001081620A2 WO2001081620A2 (de) 2001-11-01
WO2001081620A3 true WO2001081620A3 (de) 2003-02-13

Family

ID=7640438

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/001607 WO2001081620A2 (de) 2000-04-25 2001-04-25 Verfahren zur hochparallelen analyse von polymorphismen

Country Status (4)

Country Link
EP (1) EP1309728A2 (de)
AU (1) AU2001265759A1 (de)
DE (2) DE10021204A1 (de)
WO (1) WO2001081620A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10029914A1 (de) * 2000-06-19 2002-01-03 Epigenomics Ag Verfahren zur hochparallelen Analyse von Polymorphismen
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
DE10159904A1 (de) * 2001-12-06 2003-07-03 Adnagen Ag Oligonukleotidanordnung, Verfahren zum Nukleotidnachweis sowie Vorrichtung hierfür
DE102006011467B4 (de) * 2006-03-13 2019-08-29 ToposNomos Ltd. Vorrichtung und Verfahren zur Erkennung und Identifizierung von Zielstrukturen
AU2019249422A1 (en) 2018-04-02 2020-10-15 Grail, Llc Methylation markers and targeted methylation probe panels
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel

Citations (7)

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Publication number Priority date Publication date Assignee Title
US4656127A (en) * 1983-04-22 1987-04-07 Amersham International Plc. Method of detecting mutations in DNA and RNA
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
WO1997033000A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
WO1999005321A1 (en) * 1997-07-22 1999-02-04 Rapigene, Inc. Amplification and other enzymatic reactions performed on nucleic acid arrays
US5869242A (en) * 1995-09-18 1999-02-09 Myriad Genetics, Inc. Mass spectrometry to assess DNA sequence polymorphisms
EP1138782A2 (de) * 2000-03-26 2001-10-04 Bruker-Saxonia Analytik Gmbh Multiplex Sequenzvariationsanalyse von DNA-Proben mittels Massenspektrometrie

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
KR100482915B1 (ko) * 1996-01-23 2005-09-12 퀴아진 지노믹스, 인코포레이티드 크기기술을사용하여핵산분자를분석하기위한방법및조성물
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656127A (en) * 1983-04-22 1987-04-07 Amersham International Plc. Method of detecting mutations in DNA and RNA
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5869242A (en) * 1995-09-18 1999-02-09 Myriad Genetics, Inc. Mass spectrometry to assess DNA sequence polymorphisms
WO1997033000A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
WO1999005321A1 (en) * 1997-07-22 1999-02-04 Rapigene, Inc. Amplification and other enzymatic reactions performed on nucleic acid arrays
EP1138782A2 (de) * 2000-03-26 2001-10-04 Bruker-Saxonia Analytik Gmbh Multiplex Sequenzvariationsanalyse von DNA-Proben mittels Massenspektrometrie

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GONZALGO MARK L ET AL: "Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE).", NUCLEIC ACIDS RESEARCH, vol. 25, no. 12, 1997, pages 2529 - 2531, XP002208513, ISSN: 0305-1048 *
PASTINEN T ET AL: "MINISEQUENCING: A SPECIFIC TOOL FOR DNA ANALYSIS AND DIAGNOSTICS ONOLIGONUCLEOTIDE ARRAYS", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 7, no. 6, 1 June 1997 (1997-06-01), pages 606 - 614, XP000699761, ISSN: 1088-9051 *
REIN ET AL: "Identifying 5-methylcytosine and related modifications in DNA genomes", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 10, 1998, pages 2255 - 2264, XP002143106, ISSN: 0305-1048 *
TANG KAI ET AL: "Chip-based genotyping by mass spectrometry.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 18, 31 August 1999 (1999-08-31), Aug. 31, 1999, pages 10016 - 10020, XP002208514, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
DE10191554D2 (de) 2003-06-12
DE10021204A1 (de) 2001-11-08
EP1309728A2 (de) 2003-05-14
AU2001265759A1 (en) 2001-11-07
WO2001081620A2 (de) 2001-11-01

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