WO2009049007A3 - Compositions, methods and systems for rapid identification of pathogenic nucleic acids - Google Patents
Compositions, methods and systems for rapid identification of pathogenic nucleic acids Download PDFInfo
- Publication number
- WO2009049007A3 WO2009049007A3 PCT/US2008/079285 US2008079285W WO2009049007A3 WO 2009049007 A3 WO2009049007 A3 WO 2009049007A3 US 2008079285 W US2008079285 W US 2008079285W WO 2009049007 A3 WO2009049007 A3 WO 2009049007A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- compositions
- systems
- nucleic acids
- rapid identification
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Assays, methods, kits and the like are disclosed to detect the presence and type of pathogen present in a sample. In certain examples, the assays may use a universal probe effective to hybridize to a conserved region of a nucleic acid template in the sample, and a species specific probe effective to hybridize to a non-conserved region of a nucleic acid template in the sample. Methods using the assay and kits using the probes are also described.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US97903007P | 2007-10-10 | 2007-10-10 | |
US60/979,030 | 2007-10-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009049007A2 WO2009049007A2 (en) | 2009-04-16 |
WO2009049007A3 true WO2009049007A3 (en) | 2009-05-28 |
Family
ID=40549829
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/079285 WO2009049007A2 (en) | 2007-10-10 | 2008-10-09 | Compositions, methods and systems for rapid identification of pathogenic nucleic acids |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2009049007A2 (en) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
US11834720B2 (en) | 2005-10-11 | 2023-12-05 | Geneohm Sciences, Inc. | Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx |
EP2272980A1 (en) * | 2009-07-09 | 2011-01-12 | Bioesplora SRL | Molecular probes for target nucleic acid detection and quantification |
IN2012DN02587A (en) | 2009-10-22 | 2015-08-28 | Biotools Biotechnological & Medical Lab S A | |
WO2013096733A1 (en) * | 2011-12-21 | 2013-06-27 | Wake Forest University Health Sciences | Compositions and methods for detecting and identifying bacteria |
CN107727874B (en) | 2012-04-13 | 2021-03-05 | 贝克顿·迪金森公司 | Reflectance inspection of samples using previously inspected residual material |
US20150152483A1 (en) * | 2012-04-19 | 2015-06-04 | Universidad De Chile | Fast and reliable wine lactic bacteria identification method |
AU2014332956B2 (en) * | 2013-10-07 | 2018-01-25 | Mitsui Chemicals, Inc. | PCR primer set for bacterial DNA amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species |
WO2016193846A2 (en) * | 2015-05-29 | 2016-12-08 | Koninklijke Philips N.V. | Degenerate primer sets |
RU2670278C1 (en) * | 2017-12-26 | 2018-10-22 | ООО "НекстБио" | Set of synthetic ologonucleotides for quantitative determination of dna of enterobacteria, staphylococci and streptococci in mucoius membranes of vagina |
CN110964840B (en) * | 2018-09-30 | 2023-07-25 | 深圳华大因源医药科技有限公司 | Primer group, kit and library construction method for detecting 5 blood stream infection pathogens |
CN112980981B (en) * | 2021-04-26 | 2022-08-30 | 北京大学第一医院 | Primer and probe for skin infectious granulomatous pathogen, implementation method and detection system |
CN114540544A (en) * | 2021-12-24 | 2022-05-27 | 廊坊诺道中科医学检验实验室有限公司 | Primer-probe combination for detecting respiratory viruses, kit and application thereof |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20010051340A1 (en) * | 1999-04-30 | 2001-12-13 | Sharat Singh | Kits employing generalized target-binding e-tag probes |
US20020045178A1 (en) * | 2000-06-13 | 2002-04-18 | The Trustees Of Boston University | Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing |
US6403319B1 (en) * | 1999-08-13 | 2002-06-11 | Yale University | Analysis of sequence tags with hairpin primers |
US20040005555A1 (en) * | 2000-08-31 | 2004-01-08 | Rothman Richard E. | Molecular diagnosis of bactermia |
US20040191823A1 (en) * | 2001-10-16 | 2004-09-30 | Carmen Virgos | Universal e-tag primer and probe compositions and methods |
US20070105129A1 (en) * | 1994-09-12 | 2007-05-10 | Bergeron Michel G | Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
-
2008
- 2008-10-09 WO PCT/US2008/079285 patent/WO2009049007A2/en active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070105129A1 (en) * | 1994-09-12 | 2007-05-10 | Bergeron Michel G | Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
US20010051340A1 (en) * | 1999-04-30 | 2001-12-13 | Sharat Singh | Kits employing generalized target-binding e-tag probes |
US6403319B1 (en) * | 1999-08-13 | 2002-06-11 | Yale University | Analysis of sequence tags with hairpin primers |
US20020045178A1 (en) * | 2000-06-13 | 2002-04-18 | The Trustees Of Boston University | Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing |
US20040005555A1 (en) * | 2000-08-31 | 2004-01-08 | Rothman Richard E. | Molecular diagnosis of bactermia |
US20040191823A1 (en) * | 2001-10-16 | 2004-09-30 | Carmen Virgos | Universal e-tag primer and probe compositions and methods |
Also Published As
Publication number | Publication date |
---|---|
WO2009049007A2 (en) | 2009-04-16 |
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