WO2005026389A3 - Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna - Google Patents

Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna Download PDF

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Publication number
WO2005026389A3
WO2005026389A3 PCT/EP2004/009546 EP2004009546W WO2005026389A3 WO 2005026389 A3 WO2005026389 A3 WO 2005026389A3 EP 2004009546 W EP2004009546 W EP 2004009546W WO 2005026389 A3 WO2005026389 A3 WO 2005026389A3
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WO
WIPO (PCT)
Prior art keywords
genomic dna
single nucleotide
ligation
analysis
based method
Prior art date
Application number
PCT/EP2004/009546
Other languages
French (fr)
Other versions
WO2005026389A2 (en
Inventor
Aleksey Soldatov
Tatiana Borodina
Hans Lehrach
Original Assignee
Max Planck Gesellschaft
Aleksey Soldatov
Tatiana Borodina
Hans Lehrach
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Publication date
Application filed by Max Planck Gesellschaft, Aleksey Soldatov, Tatiana Borodina, Hans Lehrach filed Critical Max Planck Gesellschaft
Publication of WO2005026389A2 publication Critical patent/WO2005026389A2/en
Publication of WO2005026389A3 publication Critical patent/WO2005026389A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for the detection of the allelic state of n single nucleotide polymorphism (SNP)-loci on genomic DNA comprising contacting said genomic DNA containing SNP-loci with n detector oligonucleotide sets (DO-sets).
PCT/EP2004/009546 2003-08-29 2004-08-26 Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna WO2005026389A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03019521 2003-08-29
EP03019521.8 2003-08-29

Publications (2)

Publication Number Publication Date
WO2005026389A2 WO2005026389A2 (en) 2005-03-24
WO2005026389A3 true WO2005026389A3 (en) 2005-12-08

Family

ID=34306749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/009546 WO2005026389A2 (en) 2003-08-29 2004-08-26 Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna

Country Status (1)

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WO (1) WO2005026389A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006500034A (en) 2002-09-19 2006-01-05 アプレラ コーポレイション Methods and compositions for detecting targets
US7604937B2 (en) 2004-03-24 2009-10-20 Applied Biosystems, Llc Encoding and decoding reactions for determining target polynucleotides
ES2446927T3 (en) * 2006-03-01 2014-03-10 Keygene N.V. Rapid sequence-based SNP detection using ligation assays
CA2840929C (en) * 2011-07-08 2020-03-24 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
WO2013106807A1 (en) 2012-01-13 2013-07-18 Curry John D Scalable characterization of nucleic acids by parallel sequencing
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
WO2018229774A1 (en) * 2017-06-15 2018-12-20 Technion Research & Development Foundation Limited Compositions and methods for detection of genomic variations
CN110964791B (en) * 2019-12-26 2023-08-15 贵州中医药大学第二附属医院 Method for detecting single nucleotide polymorphism and corresponding kit

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BORODINA T A ET AL: "Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 333, no. 2, 15 October 2004 (2004-10-15), pages 309 - 319, XP004573020, ISSN: 0003-2697 *
BORODINA TATIANA A ET AL: "Ligation-based synthesis of oligonucleotides with block structure", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 318, no. 2, 15 July 2003 (2003-07-15), pages 309 - 313, XP002302313, ISSN: 0003-2697 *
GAFFNEY ROBYN ET AL: "Novel fluorescent ligase detection reaction and flow cytometric analysis of SYT-SSX fusions in synovial sarcoma.", THE JOURNAL OF MOLECULAR DIAGNOSTICS : JMD. MAY 2003, vol. 5, no. 2, May 2003 (2003-05-01), pages 127 - 135, XP002327120, ISSN: 1525-1578 *
RICKERT A M ET AL: "Refinement of single-nucleotide polymorphism genotyping methods on human genomic DNA: amplifluor allele-specific polymerase chain reaction versus ligation detection reaction-TaqMan", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 330, no. 2, 15 July 2004 (2004-07-15), pages 288 - 297, XP004519041, ISSN: 0003-2697 *
WABUYELE MUSUNDI B ET AL: "Approaching real-time molecular diagnostics: Single-pair fluorescence resonance energy transfer (spFRET) detection for the analysis of low abundant point mutations in K-ras oncogenes.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 125, no. 23, 11 June 2003 (2003-06-11), pages 6937 - 6945, XP002327119, ISSN: 0002-7863 *
WANG Y ET AL: "Microarrays assembled in microfluidic chips fabricated from poly(methyl methacrylate) for the detection of low-abundant DNA mutations", ANALYTICAL CHEMISTRY 01 MAR 2003 UNITED STATES, vol. 75, no. 5, 1 March 2003 (2003-03-01), pages 1130 - 1140, XP002327118, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
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