WO2001064945A3 - Nouvelles puces a adn - Google Patents

Nouvelles puces a adn Download PDF

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Publication number
WO2001064945A3
WO2001064945A3 PCT/FR2001/000604 FR0100604W WO0164945A3 WO 2001064945 A3 WO2001064945 A3 WO 2001064945A3 FR 0100604 W FR0100604 W FR 0100604W WO 0164945 A3 WO0164945 A3 WO 0164945A3
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WO
WIPO (PCT)
Prior art keywords
mutation
dna
chip
absence
nucleic acid
Prior art date
Application number
PCT/FR2001/000604
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English (en)
Other versions
WO2001064945A2 (fr
Inventor
Fabrice Cailloux
Original Assignee
Nucleica
Fabrice Cailloux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nucleica, Fabrice Cailloux filed Critical Nucleica
Priority to CA002401867A priority Critical patent/CA2401867A1/fr
Priority to AU2001240743A priority patent/AU2001240743A1/en
Priority to EP01911814A priority patent/EP1259644A2/fr
Priority to JP2001563632A priority patent/JP2003527846A/ja
Publication of WO2001064945A2 publication Critical patent/WO2001064945A2/fr
Publication of WO2001064945A3 publication Critical patent/WO2001064945A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

La présente invention se rapporte à un système de puces à ADN pour détecter une mutation dans une acide nucléique cible de maniéreà ce que seul l'ADN comportant la mutation demeure sur la puce à l'issu du procédé. L'invention concerne un procédé dans lequel un αS-phosphothioatedésoxynucléotide complémentaire de la mutation est ajouté au moyen d'une ADN polymérase à l'extrémité 3' de la sonde hybridée à l'acide nucléique cible et dans lequel une exonucléase est ajoutée de sorte que seules les sondes élongées ne sont pas dégradées. La détection de la présence ou de l'absence de la mutation est effectuée par mesure directe ou indirecte de la présence ou de l'absence d'ADN en un site donné sur la puce. Avantageusement, la puce comprend des transistors du type ISFET ou des transducteurs piézo-électriques.
PCT/FR2001/000604 2000-03-01 2001-03-01 Nouvelles puces a adn WO2001064945A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002401867A CA2401867A1 (fr) 2000-03-01 2001-03-01 Nouvelles puces a adn
AU2001240743A AU2001240743A1 (en) 2000-03-01 2001-03-01 Novel dna chips
EP01911814A EP1259644A2 (fr) 2000-03-01 2001-03-01 Nouvelles puces a adn
JP2001563632A JP2003527846A (ja) 2000-03-01 2001-03-01 新規なｄｎａチップ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0002614A FR2805826B1 (fr) 2000-03-01 2000-03-01 Nouvelles puces a adn
FR00/02614 2000-03-01

Publications (2)

Publication Number Publication Date
WO2001064945A2 WO2001064945A2 (fr) 2001-09-07
WO2001064945A3 true WO2001064945A3 (fr) 2002-03-28

Family

ID=8847583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2001/000604 WO2001064945A2 (fr) 2000-03-01 2001-03-01 Nouvelles puces a adn

Country Status (7)

Country Link
US (1) US20030186262A1 (fr)
EP (1) EP1259644A2 (fr)
JP (1) JP2003527846A (fr)
AU (1) AU2001240743A1 (fr)
CA (1) CA2401867A1 (fr)
FR (1) FR2805826B1 (fr)
WO (1) WO2001064945A2 (fr)

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US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP4134667A1 (fr) 2006-12-14 2023-02-15 Life Technologies Corporation Appareil permettant de mesurer des analytes en utilisant des fet arrays
JP4945279B2 (ja) * 2007-03-23 2012-06-06 株式会社日立製作所 Ｄｎａ分析方法および分析装置
RU2009146054A (ru) * 2007-05-14 2011-06-20 Инсайт Дженетикс, Инк. (Us) Способы скрининга нуклеиновых кислот для выявления одиночных нуклеотидных вариаций
JP4731544B2 (ja) * 2007-12-17 2011-07-27 株式会社日立製作所 生体分子検出装置及びそれを用いた生体分子検出方法
US8470164B2 (en) 2008-06-25 2013-06-25 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
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US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
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JP5952813B2 (ja) 2010-06-30 2016-07-13 ライフ テクノロジーズ コーポレーション Ｉｓｆｅｔアレイをテストする方法及び装置
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
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TWI794007B (zh) 2014-12-18 2023-02-21 美商生命技術公司 積體電路裝置、感測器裝置及積體電路
CN105277523B (zh) * 2015-11-27 2018-12-28 陕西师范大学 一种两亲性Eu(Ⅲ)配合物及其在检测胞嘧啶核苷三磷酸中的应用
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Citations (13)

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Publication number Priority date Publication date Assignee Title
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Also Published As

Publication number Publication date
FR2805826A1 (fr) 2001-09-07
JP2003527846A (ja) 2003-09-24
FR2805826B1 (fr) 2002-09-20
WO2001064945A2 (fr) 2001-09-07
US20030186262A1 (en) 2003-10-02
AU2001240743A1 (en) 2001-09-12
EP1259644A2 (fr) 2002-11-27
CA2401867A1 (fr) 2001-09-07

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