DE3726019A1 - Verfahren zur abtrennung und reinigung von t-pa - Google Patents

Verfahren zur abtrennung und reinigung von t-pa

Info

Publication number
DE3726019A1
DE3726019A1 DE19873726019 DE3726019A DE3726019A1 DE 3726019 A1 DE3726019 A1 DE 3726019A1 DE 19873726019 DE19873726019 DE 19873726019 DE 3726019 A DE3726019 A DE 3726019A DE 3726019 A1 DE3726019 A1 DE 3726019A1
Authority
DE
Germany
Prior art keywords
solution
elution
dye
carbohydrate
mol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19873726019
Other languages
German (de)
English (en)
Inventor
Henry W Dr Rer Nat Macartney
Josef Menhart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE19873726019 priority Critical patent/DE3726019A1/de
Priority to EP88112737A priority patent/EP0302503B1/de
Priority to DE8888112737T priority patent/DE3871277D1/de
Priority to AT88112737T priority patent/ATE76300T1/de
Priority to JP63193573A priority patent/JPS6467187A/ja
Publication of DE3726019A1 publication Critical patent/DE3726019A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pressure Welding/Diffusion-Bonding (AREA)
  • Carbon And Carbon Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
DE19873726019 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa Withdrawn DE3726019A1 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE19873726019 DE3726019A1 (de) 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa
EP88112737A EP0302503B1 (de) 1987-08-05 1988-08-04 Verfahren zur Abtrennung und Reinigung von t-PA
DE8888112737T DE3871277D1 (de) 1987-08-05 1988-08-04 Verfahren zur abtrennung und reinigung von t-pa.
AT88112737T ATE76300T1 (de) 1987-08-05 1988-08-04 Verfahren zur abtrennung und reinigung von t-pa.
JP63193573A JPS6467187A (en) 1987-08-05 1988-08-04 Method of separating and refining t-pa

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873726019 DE3726019A1 (de) 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa

Publications (1)

Publication Number Publication Date
DE3726019A1 true DE3726019A1 (de) 1989-02-16

Family

ID=6333126

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19873726019 Withdrawn DE3726019A1 (de) 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa
DE8888112737T Expired - Lifetime DE3871277D1 (de) 1987-08-05 1988-08-04 Verfahren zur abtrennung und reinigung von t-pa.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8888112737T Expired - Lifetime DE3871277D1 (de) 1987-08-05 1988-08-04 Verfahren zur abtrennung und reinigung von t-pa.

Country Status (4)

Country Link
EP (1) EP0302503B1 (enExample)
JP (1) JPS6467187A (enExample)
AT (1) ATE76300T1 (enExample)
DE (2) DE3726019A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
JP2000286602A (ja) 1999-03-30 2000-10-13 Alps Electric Co Ltd 超広帯域直線・円偏波変換器
CN100335623C (zh) * 2005-10-20 2007-09-05 上海交通大学 纤溶酶原激活剂的仿生亲和纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL191755C (nl) * 1982-10-29 1996-07-02 Mitsui Toatsu Chemicals Plasminogeenactivator en trombolytisch middel.
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
ES8702945A1 (es) * 1984-10-01 1987-01-16 Integrated Genetics Inc Un metodo de producir activador de plasminogeno de tejido uterino humano
EP0236289A3 (en) * 1986-02-26 1988-12-07 Monsanto Company Tissue plasminogen activator from normal human colon cells

Also Published As

Publication number Publication date
DE3871277D1 (de) 1992-06-25
JPH0336513B2 (enExample) 1991-05-31
EP0302503A2 (de) 1989-02-08
JPS6467187A (en) 1989-03-13
EP0302503B1 (de) 1992-05-20
EP0302503A3 (en) 1990-03-28
ATE76300T1 (de) 1992-06-15

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Legal Events

Date Code Title Description
8139 Disposal/non-payment of the annual fee