JPS6467187A - Method of separating and refining t-pa - Google Patents

Method of separating and refining t-pa

Info

Publication number
JPS6467187A
JPS6467187A JP63193573A JP19357388A JPS6467187A JP S6467187 A JPS6467187 A JP S6467187A JP 63193573 A JP63193573 A JP 63193573A JP 19357388 A JP19357388 A JP 19357388A JP S6467187 A JPS6467187 A JP S6467187A
Authority
JP
Japan
Prior art keywords
refining
separating
image
och3
purify
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP63193573A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0336513B2 (enExample
Inventor
Daburiyu Matsukaatonii Henrii
Menharuto Yoozefu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of JPS6467187A publication Critical patent/JPS6467187A/ja
Publication of JPH0336513B2 publication Critical patent/JPH0336513B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Pressure Welding/Diffusion-Bonding (AREA)
  • Carbon And Carbon Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP63193573A 1987-08-05 1988-08-04 Method of separating and refining t-pa Granted JPS6467187A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873726019 DE3726019A1 (de) 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa

Publications (2)

Publication Number Publication Date
JPS6467187A true JPS6467187A (en) 1989-03-13
JPH0336513B2 JPH0336513B2 (enExample) 1991-05-31

Family

ID=6333126

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63193573A Granted JPS6467187A (en) 1987-08-05 1988-08-04 Method of separating and refining t-pa

Country Status (4)

Country Link
EP (1) EP0302503B1 (enExample)
JP (1) JPS6467187A (enExample)
AT (1) ATE76300T1 (enExample)
DE (2) DE3726019A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
JP2000286602A (ja) 1999-03-30 2000-10-13 Alps Electric Co Ltd 超広帯域直線・円偏波変換器
CN100335623C (zh) * 2005-10-20 2007-09-05 上海交通大学 纤溶酶原激活剂的仿生亲和纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL191755C (nl) * 1982-10-29 1996-07-02 Mitsui Toatsu Chemicals Plasminogeenactivator en trombolytisch middel.
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
ES8702945A1 (es) * 1984-10-01 1987-01-16 Integrated Genetics Inc Un metodo de producir activador de plasminogeno de tejido uterino humano
EP0236289A3 (en) * 1986-02-26 1988-12-07 Monsanto Company Tissue plasminogen activator from normal human colon cells

Also Published As

Publication number Publication date
DE3871277D1 (de) 1992-06-25
JPH0336513B2 (enExample) 1991-05-31
EP0302503A2 (de) 1989-02-08
EP0302503B1 (de) 1992-05-20
DE3726019A1 (de) 1989-02-16
EP0302503A3 (en) 1990-03-28
ATE76300T1 (de) 1992-06-15

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