GB2250026A - Assays using albumin-tetrazolium interaction - Google Patents

Assays using albumin-tetrazolium interaction

Info

Publication number
GB2250026A
GB2250026A GB9121010A GB9121010A GB2250026A GB 2250026 A GB2250026 A GB 2250026A GB 9121010 A GB9121010 A GB 9121010A GB 9121010 A GB9121010 A GB 9121010A GB 2250026 A GB2250026 A GB 2250026A
Authority
GB
United Kingdom
Prior art keywords
albumin
tetrazolium
interaction
assays
tetrazolium compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9121010A
Other versions
GB9121010D0 (en
GB2250026B (en
Inventor
Anthony Atkinson
Peter Michael Hammond
Julie Miller
Christopher Philip Price
Roger James Hinton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HEALTH LAB SERVICE BOARD
Public Health Laboratory Service Board
Original Assignee
HEALTH LAB SERVICE BOARD
Public Health Laboratory Service Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HEALTH LAB SERVICE BOARD, Public Health Laboratory Service Board filed Critical HEALTH LAB SERVICE BOARD
Publication of GB9121010D0 publication Critical patent/GB9121010D0/en
Publication of GB2250026A publication Critical patent/GB2250026A/en
Application granted granted Critical
Publication of GB2250026B publication Critical patent/GB2250026B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A method for reduction of a tetrazolium compound containing the cationic nucleus (I), to produce a ring-opened formazan compound containing the group (II), in which the tetrazolium compound is caused to interact with album in and the tetrazolium compound - album in interaction product is reduced with a reducing agent in an aqueous solution at a pH between 6 and 10. The method is applied to the assay of albumin and reducing agents in samples, in which the amount of formazan produced is related to the presence and/or amount of albumin or reducing agent present. <IMAGE>
GB9121010A 1989-04-12 1991-10-03 Assays using albumin-tetrazolium interaction Expired - Fee Related GB2250026B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898908182A GB8908182D0 (en) 1989-04-12 1989-04-12 Method for assay of albumin

Publications (3)

Publication Number Publication Date
GB9121010D0 GB9121010D0 (en) 1992-01-15
GB2250026A true GB2250026A (en) 1992-05-27
GB2250026B GB2250026B (en) 1993-02-10

Family

ID=10654837

Family Applications (2)

Application Number Title Priority Date Filing Date
GB898908182A Pending GB8908182D0 (en) 1989-04-12 1989-04-12 Method for assay of albumin
GB9121010A Expired - Fee Related GB2250026B (en) 1989-04-12 1991-10-03 Assays using albumin-tetrazolium interaction

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB898908182A Pending GB8908182D0 (en) 1989-04-12 1989-04-12 Method for assay of albumin

Country Status (9)

Country Link
EP (1) EP0467938A1 (en)
JP (1) JPH04504467A (en)
KR (1) KR920701821A (en)
AU (1) AU5427990A (en)
CA (1) CA2050884A1 (en)
FI (1) FI914812A0 (en)
GB (2) GB8908182D0 (en)
HU (1) HU903644D0 (en)
WO (1) WO1990012318A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2049230C (en) * 1990-09-19 1999-10-19 Robert P. Hatch 2-benzothiazolyl tetrazolium salt indicators
US5300637A (en) * 1990-09-19 1994-04-05 Miles Inc. 2-benzothiazolyl tetrazolium salt indicators
US5126275A (en) * 1990-09-19 1992-06-30 Miles Inc. Analytical method using tetrazolium salt indicators having a reflectance plateau
GB9211980D0 (en) * 1992-06-05 1992-07-15 Int Murex Tech Corp Immunoassay method
US6225074B1 (en) * 1997-08-18 2001-05-01 Dennis Wright Direct chloramphenicol acetyl transferase assay
GB201017547D0 (en) * 2010-10-18 2010-12-01 Univ Cardiff Method and device for the detection of sulphur containing species
WO2017090631A1 (en) * 2015-11-24 2017-06-01 国立大学法人 東京大学 Fluorescent probe for detecting extracellular metabolite and screening method employing said fluorescent probe

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0279988A1 (en) * 1986-12-16 1988-08-31 Enzymatics, Inc. Digital threshold colour control system
WO1988008882A1 (en) * 1987-05-08 1988-11-17 The Public Health Laboratory Service Board Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0279988A1 (en) * 1986-12-16 1988-08-31 Enzymatics, Inc. Digital threshold colour control system
WO1988008882A1 (en) * 1987-05-08 1988-11-17 The Public Health Laboratory Service Board Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Histochemical Journal, VOL 20, 1988 pages 587-593 *

Also Published As

Publication number Publication date
GB9121010D0 (en) 1992-01-15
EP0467938A1 (en) 1992-01-29
GB2250026B (en) 1993-02-10
KR920701821A (en) 1992-08-12
CA2050884A1 (en) 1990-10-13
HU903644D0 (en) 1992-02-28
GB8908182D0 (en) 1989-05-24
AU5427990A (en) 1990-11-05
JPH04504467A (en) 1992-08-06
FI914812A0 (en) 1991-10-11
WO1990012318A1 (en) 1990-10-18

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19940411