ATE76300T1 - Verfahren zur abtrennung und reinigung von t-pa. - Google Patents

Verfahren zur abtrennung und reinigung von t-pa.

Info

Publication number
ATE76300T1
ATE76300T1 AT88112737T AT88112737T ATE76300T1 AT E76300 T1 ATE76300 T1 AT E76300T1 AT 88112737 T AT88112737 T AT 88112737T AT 88112737 T AT88112737 T AT 88112737T AT E76300 T1 ATE76300 T1 AT E76300T1
Authority
AT
Austria
Prior art keywords
purification
separation
procedure
image
och3
Prior art date
Application number
AT88112737T
Other languages
German (de)
English (en)
Inventor
Henry W Dr Rer Nat Macartney
Josef Menhart
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Application granted granted Critical
Publication of ATE76300T1 publication Critical patent/ATE76300T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pressure Welding/Diffusion-Bonding (AREA)
  • Carbon And Carbon Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
AT88112737T 1987-08-05 1988-08-04 Verfahren zur abtrennung und reinigung von t-pa. ATE76300T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19873726019 DE3726019A1 (de) 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa
EP88112737A EP0302503B1 (de) 1987-08-05 1988-08-04 Verfahren zur Abtrennung und Reinigung von t-PA

Publications (1)

Publication Number Publication Date
ATE76300T1 true ATE76300T1 (de) 1992-06-15

Family

ID=6333126

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88112737T ATE76300T1 (de) 1987-08-05 1988-08-04 Verfahren zur abtrennung und reinigung von t-pa.

Country Status (4)

Country Link
EP (1) EP0302503B1 (enExample)
JP (1) JPS6467187A (enExample)
AT (1) ATE76300T1 (enExample)
DE (2) DE3726019A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
JP2000286602A (ja) 1999-03-30 2000-10-13 Alps Electric Co Ltd 超広帯域直線・円偏波変換器
CN100335623C (zh) * 2005-10-20 2007-09-05 上海交通大学 纤溶酶原激活剂的仿生亲和纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984001714A1 (fr) * 1982-10-29 1984-05-10 Mitsui Toatsu Chemicals Nouvel activateur de plasminogene, son procede de preparation et medicament thrombolytique le contenant
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
IE81135B1 (en) * 1984-10-01 2000-03-22 Genzyme Corp Recombinant DNA techniques and the products thereof
EP0236289A3 (en) * 1986-02-26 1988-12-07 Monsanto Company Tissue plasminogen activator from normal human colon cells

Also Published As

Publication number Publication date
EP0302503A3 (en) 1990-03-28
DE3871277D1 (de) 1992-06-25
JPS6467187A (en) 1989-03-13
DE3726019A1 (de) 1989-02-16
EP0302503B1 (de) 1992-05-20
JPH0336513B2 (enExample) 1991-05-31
EP0302503A2 (de) 1989-02-08

Similar Documents

Publication Publication Date Title
ATE114172T1 (de) Reinigungsverfahren für antikörper.
ATE177100T1 (de) Verfahren zur trennung von 5-methyl- tetrahydrofolsäure
ES2091765T3 (es) Procedimiento de deteccion e identificacion de constituyentes que contienen acido nucleico.
FR2648136B1 (fr) Procede de preparation de l'acide ascorbique
MA21922A1 (fr) Procede de purification d'acides aminomethylene-phosphoniques .
DE3777611D1 (de) Lysozyme hydrochlorid und dikaliumglycyrrhizinat enthaltende loesung.
ATE98649T1 (de) Reinigung von lfa-3.
TNSN88111A1 (fr) Procede de purification d'une proteine
FI882919A0 (fi) Foerfarande.
ATE49969T1 (de) Verfahren zur herstellung von pteridinderivaten.
TNSN88080A1 (fr) Procede pour la preparation de nouveaux derives aminoalcoxyphenyle
FI850228L (fi) Substituerade 5,11-dihydro-6h-dibenz /b,e/azepin-6-oner, foerfarande foer deras framstaellning och dessa foereningar innehaollande laekemedel.
ATE76300T1 (de) Verfahren zur abtrennung und reinigung von t-pa.
FR2565967B1 (fr) Procede de preparation d'anhydride trifluoracetique
ATE97449T1 (de) Methode zur reinigung und isolierung von zwei ionischen formen von somatomedin.
EP0608693A3 (de) Verfahren zur Herstellung von Folsäure.
DE3855002D1 (de) Verfahren zur Herstellung von Dodecahydro-3a,6,6,9a-tetramethylnaphto(2,1-b)furan
ES2079041T3 (es) Preparacion de 2-(2'-tienil)alquilaminas y sus derivados y sintesis de 4,5,6,7-tieno(3,2-c)piridina derivados de estos.
RU1833365C (ru) Cпocoб пoлучehия tpииoдбehзoльhыx coeдиhehий
FR2644474B1 (fr) Procede de purification d'enzymes
ATE76082T1 (de) Reinigungsmethode fuer antikoerper.
ES2063890T3 (es) Derivados y analogos de monoetilglicinaxilidina y su produccion y uso.
DE69015907D1 (de) Verfahren zur optischen Trennung von Dropropizin.
BE873659A (fr) Procede de production de l'acide 4-amino-butyrique ou de ses derives
DE69105098D1 (de) Radioaktive Jodverbindung zur Markierung von Antikörpern.

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee