DE3780883T2 - Verfahren zur reinigung von tpa aus rohen praeparaten. - Google Patents
Verfahren zur reinigung von tpa aus rohen praeparaten.Info
- Publication number
- DE3780883T2 DE3780883T2 DE8787307089T DE3780883T DE3780883T2 DE 3780883 T2 DE3780883 T2 DE 3780883T2 DE 8787307089 T DE8787307089 T DE 8787307089T DE 3780883 T DE3780883 T DE 3780883T DE 3780883 T2 DE3780883 T2 DE 3780883T2
- Authority
- DE
- Germany
- Prior art keywords
- purifying tpa
- raw preparations
- preparations
- raw
- tpa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61186851A JP2507339B2 (ja) | 1986-08-11 | 1986-08-11 | 粗tPAの精製方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3780883D1 DE3780883D1 (de) | 1992-09-10 |
DE3780883T2 true DE3780883T2 (de) | 1993-02-04 |
Family
ID=16195757
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE8787307089T Expired - Fee Related DE3780883T2 (de) | 1986-08-11 | 1987-08-11 | Verfahren zur reinigung von tpa aus rohen praeparaten. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5141863A (de) |
EP (1) | EP0256836B1 (de) |
JP (1) | JP2507339B2 (de) |
AU (1) | AU586756B2 (de) |
CA (1) | CA1302928C (de) |
DE (1) | DE3780883T2 (de) |
DK (1) | DK169680B1 (de) |
FI (1) | FI88308C (de) |
NO (1) | NO175011C (de) |
ZA (1) | ZA875902B (de) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01238534A (ja) * | 1988-03-17 | 1989-09-22 | Mitsui Toatsu Chem Inc | エンドトキシンの除去方法 |
US7544500B2 (en) | 1999-11-13 | 2009-06-09 | Talecris Biotherapeutics, Inc. | Process for the production of a reversibly inactive acidified plasmin composition |
US6355243B1 (en) | 1999-11-13 | 2002-03-12 | Bayer Corporation | Method of thrombolysis by local delivery of active plasmin |
US20020064860A1 (en) * | 2000-11-29 | 2002-05-30 | Schering Corporation | Method for purifying adenoviruses |
EP2238154B1 (de) | 2008-01-18 | 2015-09-16 | Bio-Rad Laboratories, Inc. | Erweiterte reinigung phosphorylierter und nicht phosphorylierter biomoleküle durch apatit-chromatographie |
RU2497948C2 (ru) | 2008-06-04 | 2013-11-10 | Тэйлкрис Байотерапьютикс, Инк. | Композиция, способ и набор для получения плазмина |
ES2552337T3 (es) | 2009-03-03 | 2015-11-27 | Grifols Therapeutics Inc. | Procedimientos para la preparación de plasminógeno |
CA2787009C (en) | 2010-01-15 | 2018-04-03 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
US9592540B2 (en) | 2011-02-02 | 2017-03-14 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
CN102286449B (zh) * | 2011-05-30 | 2013-04-03 | 云南省地方病防治所 | 鼠疫菌纤溶酶原激活因子Pla的提取与纯化方法 |
CN104507952B (zh) | 2012-05-30 | 2018-08-10 | 生物辐射实验室股份有限公司 | 基于磷灰石的色谱树脂的原位恢复 |
EP3157551B1 (de) | 2014-06-23 | 2023-02-15 | Bio-Rad Laboratories, Inc. | In-situ-restaurierung von apatit |
EP3157689B1 (de) | 2014-06-23 | 2021-04-28 | Bio-Rad Laboratories, Inc. | Apatit-vorbehandlung |
CN111909920B (zh) * | 2020-08-24 | 2022-04-15 | 武汉人福药业有限责任公司 | 一种重组人组织型纤溶酶原激活剂的表达纯化方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3542646A (en) * | 1966-11-22 | 1970-11-24 | Green Cross Corp | Process for fractionally obtaining urokinase and blood coagulation accelerator in human urine |
IL43343A (en) * | 1972-10-24 | 1976-03-31 | Lilly Co Eli | Production of plasminogen activator |
US3904480A (en) * | 1972-10-24 | 1975-09-09 | Lilly Co Eli | Enhanced production of plasminogen activator |
JPS55130918A (en) * | 1979-03-30 | 1980-10-11 | Toubishi Yakuhin Kogyo Kk | Plasminogen-activating agent and its preparation |
JPS57146580A (en) * | 1981-03-09 | 1982-09-10 | Seikagaku Kogyo Co Ltd | Separation and purification of enzyme in human urine |
DE3382389D1 (de) * | 1982-12-14 | 1991-10-02 | South African Inventions | Plasminogenaktivator. |
US4960702A (en) * | 1985-09-06 | 1990-10-02 | Codon | Methods for recovery of tissue plasminogen activator |
US4898825A (en) * | 1986-05-07 | 1990-02-06 | Mitsui Toatsu Chemicals, Incorporated | Methods for purification of single-chain and double-chain tissue plasminogen activator |
JPS6379591A (ja) * | 1986-09-22 | 1988-04-09 | Mitsui Toatsu Chem Inc | tPAの精製方法 |
US4928620A (en) * | 1988-09-01 | 1990-05-29 | Currey Lesley B | Seat pedestal mount |
-
1986
- 1986-08-11 JP JP61186851A patent/JP2507339B2/ja not_active Expired - Lifetime
-
1987
- 1987-08-07 AU AU76666/87A patent/AU586756B2/en not_active Ceased
- 1987-08-10 NO NO873332A patent/NO175011C/no unknown
- 1987-08-10 CA CA000544117A patent/CA1302928C/en not_active Expired - Fee Related
- 1987-08-10 FI FI873470A patent/FI88308C/fi not_active IP Right Cessation
- 1987-08-10 ZA ZA875902A patent/ZA875902B/xx unknown
- 1987-08-11 DE DE8787307089T patent/DE3780883T2/de not_active Expired - Fee Related
- 1987-08-11 EP EP87307089A patent/EP0256836B1/de not_active Expired - Lifetime
- 1987-08-11 DK DK418487A patent/DK169680B1/da active
-
1990
- 1990-05-01 US US07/517,383 patent/US5141863A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0256836B1 (de) | 1992-08-05 |
DK169680B1 (da) | 1995-01-09 |
AU7666687A (en) | 1988-02-18 |
DE3780883D1 (de) | 1992-09-10 |
CA1302928C (en) | 1992-06-09 |
NO175011C (no) | 1994-08-17 |
FI88308B (fi) | 1993-01-15 |
NO873332L (no) | 1988-02-12 |
US5141863A (en) | 1992-08-25 |
DK418487D0 (da) | 1987-08-11 |
ZA875902B (en) | 1988-02-12 |
FI873470A (fi) | 1988-02-12 |
AU586756B2 (en) | 1989-07-20 |
EP0256836A1 (de) | 1988-02-24 |
NO873332D0 (no) | 1987-08-10 |
DK418487A (da) | 1988-02-12 |
NO175011B (no) | 1994-05-09 |
JPS6342687A (ja) | 1988-02-23 |
FI873470A0 (fi) | 1987-08-10 |
JP2507339B2 (ja) | 1996-06-12 |
FI88308C (fi) | 1993-04-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |