JPS57146580A - Separation and purification of enzyme in human urine - Google Patents
Separation and purification of enzyme in human urineInfo
- Publication number
- JPS57146580A JPS57146580A JP56032547A JP3254781A JPS57146580A JP S57146580 A JPS57146580 A JP S57146580A JP 56032547 A JP56032547 A JP 56032547A JP 3254781 A JP3254781 A JP 3254781A JP S57146580 A JPS57146580 A JP S57146580A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- eluted
- elastin
- human urine
- hydroxyapatite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PURPOSE: To separate and to purify UK AND UP from human urine industrially advantageously, by combining methods using hydroxyapatite, elastin, and a weakly basic anion exchanger as adsorbents.
CONSTITUTION: A solution containing UK and UP, especially human urine is brought into contact with hydroxyapatite, eluted with a buffer solution of a phosphate, tris hydrochloride buffer solution, etc. at a pH of about neutrality, hving a concentration of 2/100W20/100M, ammonium sulfate is added to the eluted solution to make a saturation of 0.2W0.5, the solution is brought into contact with elastin, and UK is conventionally obtained from the passed solution. While elastin is eluted with a buffere solution at a pH of slight acidityW neutrality, having a concentration of 1/200W5/100M, the eluted solution is subjected to ion exchange chromatography using a weakly basic ion exchanger such as DEAE-Sephadex, DEAP-cellulose, etc., and subjected to gel filtration to give UP.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56032547A JPS57146580A (en) | 1981-03-09 | 1981-03-09 | Separation and purification of enzyme in human urine |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56032547A JPS57146580A (en) | 1981-03-09 | 1981-03-09 | Separation and purification of enzyme in human urine |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57146580A true JPS57146580A (en) | 1982-09-10 |
Family
ID=12361952
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56032547A Pending JPS57146580A (en) | 1981-03-09 | 1981-03-09 | Separation and purification of enzyme in human urine |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57146580A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61251700A (en) * | 1985-02-12 | 1986-11-08 | ジエネンテク,インコ−ポレイテツド | Insulin receptor |
JPS6342687A (en) * | 1986-08-11 | 1988-02-23 | Mitsui Toatsu Chem Inc | Purification of crude tpa |
JPH05163158A (en) * | 1991-12-13 | 1993-06-29 | Sanwa Kagaku Kenkyusho Co Ltd | Agent for prevention and treatment of skin sore containing human urinary kininogenase as active component |
-
1981
- 1981-03-09 JP JP56032547A patent/JPS57146580A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61251700A (en) * | 1985-02-12 | 1986-11-08 | ジエネンテク,インコ−ポレイテツド | Insulin receptor |
JPS6342687A (en) * | 1986-08-11 | 1988-02-23 | Mitsui Toatsu Chem Inc | Purification of crude tpa |
JPH05163158A (en) * | 1991-12-13 | 1993-06-29 | Sanwa Kagaku Kenkyusho Co Ltd | Agent for prevention and treatment of skin sore containing human urinary kininogenase as active component |
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