JPS57146580A - Separation and purification of enzyme in human urine - Google Patents

Separation and purification of enzyme in human urine

Info

Publication number
JPS57146580A
JPS57146580A JP56032547A JP3254781A JPS57146580A JP S57146580 A JPS57146580 A JP S57146580A JP 56032547 A JP56032547 A JP 56032547A JP 3254781 A JP3254781 A JP 3254781A JP S57146580 A JPS57146580 A JP S57146580A
Authority
JP
Japan
Prior art keywords
solution
eluted
elastin
human urine
hydroxyapatite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56032547A
Other languages
Japanese (ja)
Inventor
Takehiko Yamamoto
Yoshichika Minamiura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP56032547A priority Critical patent/JPS57146580A/en
Publication of JPS57146580A publication Critical patent/JPS57146580A/en
Pending legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE: To separate and to purify UK AND UP from human urine industrially advantageously, by combining methods using hydroxyapatite, elastin, and a weakly basic anion exchanger as adsorbents.
CONSTITUTION: A solution containing UK and UP, especially human urine is brought into contact with hydroxyapatite, eluted with a buffer solution of a phosphate, tris hydrochloride buffer solution, etc. at a pH of about neutrality, hving a concentration of 2/100W20/100M, ammonium sulfate is added to the eluted solution to make a saturation of 0.2W0.5, the solution is brought into contact with elastin, and UK is conventionally obtained from the passed solution. While elastin is eluted with a buffere solution at a pH of slight acidityW neutrality, having a concentration of 1/200W5/100M, the eluted solution is subjected to ion exchange chromatography using a weakly basic ion exchanger such as DEAE-Sephadex, DEAP-cellulose, etc., and subjected to gel filtration to give UP.
COPYRIGHT: (C)1982,JPO&Japio
JP56032547A 1981-03-09 1981-03-09 Separation and purification of enzyme in human urine Pending JPS57146580A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56032547A JPS57146580A (en) 1981-03-09 1981-03-09 Separation and purification of enzyme in human urine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56032547A JPS57146580A (en) 1981-03-09 1981-03-09 Separation and purification of enzyme in human urine

Publications (1)

Publication Number Publication Date
JPS57146580A true JPS57146580A (en) 1982-09-10

Family

ID=12361952

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56032547A Pending JPS57146580A (en) 1981-03-09 1981-03-09 Separation and purification of enzyme in human urine

Country Status (1)

Country Link
JP (1) JPS57146580A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61251700A (en) * 1985-02-12 1986-11-08 ジエネンテク，インコ−ポレイテツド Insulin receptor
JPS6342687A (en) * 1986-08-11 1988-02-23 Mitsui Toatsu Chem Inc Purification of crude tpa
JPH05163158A (en) * 1991-12-13 1993-06-29 Sanwa Kagaku Kenkyusho Co Ltd Agent for prevention and treatment of skin sore containing human urinary kininogenase as active component

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61251700A (en) * 1985-02-12 1986-11-08 ジエネンテク，インコ−ポレイテツド Insulin receptor
JPS6342687A (en) * 1986-08-11 1988-02-23 Mitsui Toatsu Chem Inc Purification of crude tpa
JPH05163158A (en) * 1991-12-13 1993-06-29 Sanwa Kagaku Kenkyusho Co Ltd Agent for prevention and treatment of skin sore containing human urinary kininogenase as active component

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