DE2512606A1 - Verfahren zur herstellung eines cholesterin-oxidase-enzyms - Google Patents
Verfahren zur herstellung eines cholesterin-oxidase-enzymsInfo
- Publication number
- DE2512606A1 DE2512606A1 DE19752512606 DE2512606A DE2512606A1 DE 2512606 A1 DE2512606 A1 DE 2512606A1 DE 19752512606 DE19752512606 DE 19752512606 DE 2512606 A DE2512606 A DE 2512606A DE 2512606 A1 DE2512606 A1 DE 2512606A1
- Authority
- DE
- Germany
- Prior art keywords
- cholesterol
- medium
- enzyme
- cells
- carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 108010085346 steroid delta-isomerase Proteins 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/872—Nocardia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US454658A US3909359A (en) | 1974-03-25 | 1974-03-25 | Method for the preparation of cholesterol oxidase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2512606A1 true DE2512606A1 (de) | 1975-10-02 |
Family
ID=23805538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752512606 Pending DE2512606A1 (de) | 1974-03-25 | 1975-03-21 | Verfahren zur herstellung eines cholesterin-oxidase-enzyms |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US3909359A (cg-RX-API-DMAC7.html) |
| JP (1) | JPS5646829B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA1043723A (cg-RX-API-DMAC7.html) |
| DE (1) | DE2512606A1 (cg-RX-API-DMAC7.html) |
| FR (1) | FR2265858B1 (cg-RX-API-DMAC7.html) |
| GB (1) | GB1494030A (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2656063A1 (de) * | 1975-12-11 | 1977-07-07 | Eastman Kodak Co | Verfahren zur herstellung von cholesterin-oxidase |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2456586C3 (de) * | 1974-11-29 | 1979-09-27 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verwendung von bestimmten Mikroorganismenstämmen zur Gewinnung von Cholesterinoxidase, welche Cholesterin mit O2 zu Cholestenon + H2 O2 oxidiert |
| US4081363A (en) * | 1975-05-29 | 1978-03-28 | American Cyanamid Company | Mineral beneficiation by froth flotation: use of alcohol ethoxylate partial esters of polycarboxylic acids |
| JPS527484A (en) * | 1975-07-04 | 1977-01-20 | Kikkoman Corp | Process for preparing cholesterol oxidase |
| US4035237A (en) * | 1975-11-07 | 1977-07-12 | Eastman Kodak Company | Method for the preparation of cholesterol oxidase |
| US4052263A (en) * | 1975-12-11 | 1977-10-04 | Eastman Kodak Company | Production of cholesterol esterase using Nocardia cholesterolicum |
| US5238816A (en) * | 1989-07-24 | 1993-08-24 | Asahi Kasei Kogyo Kabushiki Kaisha | Omega carboxyalcohol oxidase enzyme |
| JP2786679B2 (ja) * | 1989-07-24 | 1998-08-13 | 旭化成工業株式会社 | 新規なω位カルボキシアルコール酸化酵素 |
| US5206148A (en) * | 1989-07-24 | 1993-04-27 | Asahi Kasei Kogyo Kabushiki Kaisha | Method for assaying aliphatic alcohol, aliphatic aldehyde or ω-carboxylic acid derivatives thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2047147A5 (cg-RX-API-DMAC7.html) * | 1969-05-06 | 1971-03-12 | Kyowa Hakko Kogyo Kk |
-
1974
- 1974-03-25 US US454658A patent/US3909359A/en not_active Expired - Lifetime
-
1975
- 1975-02-19 CA CA220,412A patent/CA1043723A/en not_active Expired
- 1975-03-21 DE DE19752512606 patent/DE2512606A1/de active Pending
- 1975-03-24 GB GB12203/75A patent/GB1494030A/en not_active Expired
- 1975-03-25 FR FR7509198A patent/FR2265858B1/fr not_active Expired
- 1975-03-25 JP JP3504375A patent/JPS5646829B2/ja not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2656063A1 (de) * | 1975-12-11 | 1977-07-07 | Eastman Kodak Co | Verfahren zur herstellung von cholesterin-oxidase |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2265858B1 (cg-RX-API-DMAC7.html) | 1977-11-25 |
| GB1494030A (en) | 1977-12-07 |
| CA1043723A (en) | 1978-12-05 |
| JPS5646829B2 (cg-RX-API-DMAC7.html) | 1981-11-05 |
| US3909359A (en) | 1975-09-30 |
| JPS50135278A (cg-RX-API-DMAC7.html) | 1975-10-27 |
| FR2265858A1 (cg-RX-API-DMAC7.html) | 1975-10-24 |
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