DE102012213838A1 - Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit - Google Patents

Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit Download PDF

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Publication number
DE102012213838A1
DE102012213838A1 DE102012213838.2A DE102012213838A DE102012213838A1 DE 102012213838 A1 DE102012213838 A1 DE 102012213838A1 DE 102012213838 A DE102012213838 A DE 102012213838A DE 102012213838 A1 DE102012213838 A1 DE 102012213838A1
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cells
tumor cells
tumor
spheroids
body fluid
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English (en)
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Katharina Pachmann
Ulrich Pachmann
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Priority to DE102012213838.2A priority Critical patent/DE102012213838A1/de
Priority to IN1407DEN2015 priority patent/IN2015DN01407A/en
Priority to AU2013298450A priority patent/AU2013298450B2/en
Priority to SI201331064T priority patent/SI2880152T1/sl
Priority to CN201380050740.XA priority patent/CN104685051A/zh
Priority to HRP20180905TT priority patent/HRP20180905T1/hr
Priority to EP18152803.5A priority patent/EP3330373A1/de
Priority to TR2018/08441T priority patent/TR201808441T4/tr
Priority to PCT/EP2013/066325 priority patent/WO2014020169A1/de
Priority to PL13745085T priority patent/PL2880152T3/pl
Priority to ES13745085.4T priority patent/ES2673874T3/es
Priority to US14/419,149 priority patent/US11015172B2/en
Priority to DK13745085.4T priority patent/DK2880152T3/en
Priority to EP13745085.4A priority patent/EP2880152B1/de
Priority to PT137450854T priority patent/PT2880152T/pt
Priority to HUE13745085A priority patent/HUE037771T2/hu
Publication of DE102012213838A1 publication Critical patent/DE102012213838A1/de
Priority to US17/233,783 priority patent/US11920163B2/en
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • C12N5/0694Cells of blood, e.g. leukemia cells, myeloma cells
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • C12N5/0695Stem cells; Progenitor cells; Precursor cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/11Epidermal growth factor [EGF]
    • CCHEMISTRY; METALLURGY
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062General methods for three-dimensional culture
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/30Electrochemically active labels

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DE102012213838.2A 2012-08-03 2012-08-03 Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit Withdrawn DE102012213838A1 (de)

Priority Applications (17)

Application Number Priority Date Filing Date Title
DE102012213838.2A DE102012213838A1 (de) 2012-08-03 2012-08-03 Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit
PL13745085T PL2880152T3 (pl) 2012-08-03 2013-08-02 Sposób hodowli subpopulacji krążących nabłonkowych komórek nowotworowych z płynu ustrojowego
ES13745085.4T ES2673874T3 (es) 2012-08-03 2013-08-02 Procedimiento para el cultivo de una subpoblación de células tumorales epiteliales circulantes de un líquido corporal
SI201331064T SI2880152T1 (sl) 2012-08-03 2013-08-02 Postopek gojenja podpopulacije krožečih epitelijskih tumorskih celic iz telesne tekočine
CN201380050740.XA CN104685051A (zh) 2012-08-03 2013-08-02 用于对来自体液的循环上皮肿瘤细胞亚群体进行培养的方法
HRP20180905TT HRP20180905T1 (hr) 2012-08-03 2013-08-02 Postupak uzgoja subpopulacije cirkulirajućih epitelnih tumorskih stanica iz tjelesne tekućine
EP18152803.5A EP3330373A1 (de) 2012-08-03 2013-08-02 Verfahren zur kultivierung einer subpopulation zirkulierender epithelialer tumorzellen aus einer körperflüssigkeit
TR2018/08441T TR201808441T4 (tr) 2012-08-03 2013-08-02 Bir vücut sıvısından bir sirküle epitelyal tümör hücreleri alt popülasyonunun kültivasyonu için yöntem.
PCT/EP2013/066325 WO2014020169A1 (de) 2012-08-03 2013-08-02 Verfahren zur kultivierung einer subpopulation zirkulierender epithelialer tumorzellen aus einer körperflüssigkeit
IN1407DEN2015 IN2015DN01407A (enExample) 2012-08-03 2013-08-02
AU2013298450A AU2013298450B2 (en) 2012-08-03 2013-08-02 Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid
US14/419,149 US11015172B2 (en) 2012-08-03 2013-08-02 Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid
DK13745085.4T DK2880152T3 (en) 2012-08-03 2013-08-02 Method for Culturing a Subpopulation of Circulating Epithelial Tumor Cells from a Body Fluid
EP13745085.4A EP2880152B1 (de) 2012-08-03 2013-08-02 Verfahren zur kultivierung einer subpopulation zirkulierender epithelialer tumorzellen aus einer körperflüssigkeit
PT137450854T PT2880152T (pt) 2012-08-03 2013-08-02 Método para o cultivo de uma subpopulação de células tumorais epiteliais circulantes a partir de um fluido corporal
HUE13745085A HUE037771T2 (hu) 2012-08-03 2013-08-02 Eljárás testfolyadékból származó keringõ epitéliás tumor sejtek tenyésztésére
US17/233,783 US11920163B2 (en) 2012-08-03 2021-04-19 Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102012213838.2A DE102012213838A1 (de) 2012-08-03 2012-08-03 Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit

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DE102012213838.2A Withdrawn DE102012213838A1 (de) 2012-08-03 2012-08-03 Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit

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US (2) US11015172B2 (enExample)
EP (2) EP3330373A1 (enExample)
CN (1) CN104685051A (enExample)
AU (1) AU2013298450B2 (enExample)
DE (1) DE102012213838A1 (enExample)
DK (1) DK2880152T3 (enExample)
ES (1) ES2673874T3 (enExample)
HR (1) HRP20180905T1 (enExample)
HU (1) HUE037771T2 (enExample)
IN (1) IN2015DN01407A (enExample)
PL (1) PL2880152T3 (enExample)
PT (1) PT2880152T (enExample)
SI (1) SI2880152T1 (enExample)
TR (1) TR201808441T4 (enExample)
WO (1) WO2014020169A1 (enExample)

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SG10201707902PA (en) 2014-03-31 2017-11-29 Fisher & Paykel Healthcare Ltd Headgear for respiratory interfaces
EP3128325B1 (de) 2015-08-07 2019-01-02 Ulrich Pachmann Verfahren zur bestimmung einer konzentration epithelialer zellen in einer blutprobe oder aspiratsprobe
CN109196112B (zh) * 2016-06-16 2022-10-04 株式会社日立高新技术 球状体内部细胞状态的分析方法
CN108277255A (zh) * 2018-01-30 2018-07-13 陈晓凤 一种体外活性上皮肿瘤细胞的检测方法
JP6935883B2 (ja) * 2018-07-13 2021-09-15 五稜化薬株式会社 蛍光画像診断の前処理方法
KR102837377B1 (ko) * 2020-12-31 2025-07-23 아카데미아 시니카 세포 배양 시스템, 이의 제조방법 및 용도
US11713441B2 (en) 2020-12-31 2023-08-01 Academia Sinica Cell culture substrates, methods and uses thereof

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Publication number Publication date
AU2013298450A1 (en) 2015-03-12
WO2014020169A1 (de) 2014-02-06
DK2880152T3 (en) 2018-06-25
HRP20180905T1 (hr) 2018-08-24
EP2880152B1 (de) 2018-03-14
HUE037771T2 (hu) 2018-09-28
US11015172B2 (en) 2021-05-25
PL2880152T3 (pl) 2018-10-31
TR201808441T4 (tr) 2018-07-23
IN2015DN01407A (enExample) 2015-07-03
CN104685051A (zh) 2015-06-03
EP3330373A1 (de) 2018-06-06
EP2880152A1 (de) 2015-06-10
PT2880152T (pt) 2018-06-20
US20150299663A1 (en) 2015-10-22
ES2673874T3 (es) 2018-06-26
SI2880152T1 (sl) 2018-09-28
US20210238554A1 (en) 2021-08-05
AU2013298450B2 (en) 2019-01-17
US11920163B2 (en) 2024-03-05

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