IN2015DN01407A - - Google Patents

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Publication number
IN2015DN01407A
IN2015DN01407A IN1407DEN2015A IN2015DN01407A IN 2015DN01407 A IN2015DN01407 A IN 2015DN01407A IN 1407DEN2015 A IN1407DEN2015 A IN 1407DEN2015A IN 2015DN01407 A IN2015DN01407 A IN 2015DN01407A
Authority
IN
India
Prior art keywords
body fluid
epithelial tumour
cells
spheroids
subpopulation
Prior art date
Application number
Inventor
Ulrich Pachmann
Katharina Pachmann
Original Assignee
Ulrich Pachmann
Katharina Pachmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ulrich Pachmann, Katharina Pachmann filed Critical Ulrich Pachmann
Publication of IN2015DN01407A publication Critical patent/IN2015DN01407A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/30—Electrochemically active labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid of a human or animal suffering from an epithelial tumour wherein cells contained in the body fluid each containing at least one cell nucleus are separated from the body fluid and cultured over at least 24 hours in suspension with formation of spheroids.
IN1407DEN2015 2012-08-03 2013-08-02 IN2015DN01407A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012213838.2A DE102012213838A1 (en) 2012-08-03 2012-08-03 A method of culturing a subpopulation of circulating epithelial tumor cells from a body fluid
PCT/EP2013/066325 WO2014020169A1 (en) 2012-08-03 2013-08-02 Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid

Publications (1)

Publication Number Publication Date
IN2015DN01407A true IN2015DN01407A (en) 2015-07-03

Family

ID=48916061

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1407DEN2015 IN2015DN01407A (en) 2012-08-03 2013-08-02

Country Status (15)

Country Link
US (2) US11015172B2 (en)
EP (2) EP2880152B1 (en)
CN (1) CN104685051A (en)
AU (1) AU2013298450B2 (en)
DE (1) DE102012213838A1 (en)
DK (1) DK2880152T3 (en)
ES (1) ES2673874T3 (en)
HR (1) HRP20180905T1 (en)
HU (1) HUE037771T2 (en)
IN (1) IN2015DN01407A (en)
PL (1) PL2880152T3 (en)
PT (1) PT2880152T (en)
SI (1) SI2880152T1 (en)
TR (1) TR201808441T4 (en)
WO (1) WO2014020169A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3128325T3 (en) 2015-08-07 2019-04-15 Ulrich Pachmann PROCEDURE FOR DETERMINING A CONCENTRATION OF EPITHELIAL CELLS IN A BLOOD OR ASPIRATE SAMPLE
EP3473727B1 (en) * 2016-06-16 2022-01-19 Hitachi High-Tech Corporation Method for analyzing state of cells in spheroid
CN108277255A (en) * 2018-01-30 2018-07-13 陈晓凤 A kind of detection method of external activity epithelial tumor cell
JP6935883B2 (en) * 2018-07-13 2021-09-15 五稜化薬株式会社 Pretreatment method for fluorescence image diagnosis
US11713441B2 (en) 2020-12-31 2023-08-01 Academia Sinica Cell culture substrates, methods and uses thereof
AU2021411588B2 (en) * 2020-12-31 2023-03-02 Academia Sinica Cell culture substrates, methods and uses thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
CN1446583A (en) * 2002-11-29 2003-10-08 帕弗瑞生物技术(北京)有限公司 Composition, preparation and application scheme of tumor immunological therapy and preventative vaccine
EP1664340A1 (en) * 2003-08-08 2006-06-07 Canbas Co., Ltd. Sensitivity test to predict efficacy of anti-cancer therapies
US20070123448A1 (en) * 2005-11-23 2007-05-31 The Hospital For Sick Children Novel chemical entities affecting neuroblastoma tumor-initiating cells
FI20075417A0 (en) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Formulations and methods for culturing embryonic stem cells
EP2138571B1 (en) * 2008-06-26 2017-04-12 SpheroTec GmbH Process for the preparation of multicellular spheroids
DE102009047146B4 (en) * 2009-11-25 2012-07-19 Ulrich Pachmann A method for predicting the response of a tumor disease to a therapeutic intervention
US20130078667A1 (en) * 2011-09-22 2013-03-28 Oscar B. Goodman Methods for detecting and collecting circulating tumor cells
EP2785871A4 (en) * 2011-11-28 2015-08-26 Baylor College Medicine A ctc biomarker assay to combat breast cancer brain metastasis
CN108165603A (en) * 2013-01-25 2018-06-15 艾克斯赛尔生物科学公司 For the method for selective enrichment target cell, composition, kit and system

Also Published As

Publication number Publication date
US20150299663A1 (en) 2015-10-22
HUE037771T2 (en) 2018-09-28
SI2880152T1 (en) 2018-09-28
US20210238554A1 (en) 2021-08-05
DE102012213838A1 (en) 2014-02-06
HRP20180905T1 (en) 2018-08-24
US11920163B2 (en) 2024-03-05
EP2880152A1 (en) 2015-06-10
PT2880152T (en) 2018-06-20
US11015172B2 (en) 2021-05-25
EP3330373A1 (en) 2018-06-06
WO2014020169A1 (en) 2014-02-06
EP2880152B1 (en) 2018-03-14
TR201808441T4 (en) 2018-07-23
CN104685051A (en) 2015-06-03
ES2673874T3 (en) 2018-06-26
DK2880152T3 (en) 2018-06-25
AU2013298450A1 (en) 2015-03-12
AU2013298450B2 (en) 2019-01-17
PL2880152T3 (en) 2018-10-31

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