RU2013151490A - METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1 - Google Patents

METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1 Download PDF

Info

Publication number
RU2013151490A
RU2013151490A RU2013151490/10A RU2013151490A RU2013151490A RU 2013151490 A RU2013151490 A RU 2013151490A RU 2013151490/10 A RU2013151490/10 A RU 2013151490/10A RU 2013151490 A RU2013151490 A RU 2013151490A RU 2013151490 A RU2013151490 A RU 2013151490A
Authority
RU
Russia
Prior art keywords
synchronizing
stage
cell cycle
fibroblasts
issued
Prior art date
Application number
RU2013151490/10A
Other languages
Russian (ru)
Inventor
Исрафиль Назбиевич Чаушев
Аминат Исрафиловна Чаушева
Original Assignee
Исрафиль Назбиевич Чаушев
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Исрафиль Назбиевич Чаушев filed Critical Исрафиль Назбиевич Чаушев
Priority to RU2013151490/10A priority Critical patent/RU2013151490A/en
Publication of RU2013151490A publication Critical patent/RU2013151490A/en

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Способ синхронизации клеточного цикла фибробластов, выделенных из кожи человека, на стадии G/G, заключающийся в том, что клетки культивируют в сыворотке плодов коров, отличающийся тем, что сыворотку плодов коров исключают из среды культивирования на момент образования 70% монослоя культивируемых клеток, с дальнейшим культивированием в течение 48 ч.The method of synchronizing the cell cycle of fibroblasts isolated from human skin, at the stage of G / G, namely, that the cells are cultured in the serum of cow fruits, characterized in that the serum of cow fruits is excluded from the culture medium at the time of formation of 70% of the monolayer of cultured cells, further cultivation for 48 hours

Claims (1)

Способ синхронизации клеточного цикла фибробластов, выделенных из кожи человека, на стадии G0/G1, заключающийся в том, что клетки культивируют в сыворотке плодов коров, отличающийся тем, что сыворотку плодов коров исключают из среды культивирования на момент образования 70% монослоя культивируемых клеток, с дальнейшим культивированием в течение 48 ч. The method of synchronizing the cell cycle of fibroblasts isolated from human skin, at the stage G 0 / G 1 , which consists in the fact that the cells are cultured in the serum of cow fruits, characterized in that the serum of cow fruits is excluded from the culture medium at the time of formation of 70% of the monolayer of cultured cells , with further cultivation for 48 hours
RU2013151490/10A 2013-11-19 2013-11-19 METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1 RU2013151490A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2013151490/10A RU2013151490A (en) 2013-11-19 2013-11-19 METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2013151490/10A RU2013151490A (en) 2013-11-19 2013-11-19 METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1

Publications (1)

Publication Number Publication Date
RU2013151490A true RU2013151490A (en) 2015-05-27

Family

ID=53284825

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2013151490/10A RU2013151490A (en) 2013-11-19 2013-11-19 METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1

Country Status (1)

Country Link
RU (1) RU2013151490A (en)

Similar Documents

Publication Publication Date Title
TW201643242A (en) Systems and methods for growing and harvesting cells
MX2017004890A (en) Methods and compositions for generating or maintaining pluripotent cells.
MX2016008251A (en) Media for cell culture.
TR201904270T4 (en) Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions.
MX2018014043A (en) Cell culture compositions with antioxidants and methods for polypeptide production.
MX2015012321A (en) Chlorophyll deficient algal cell with improved growth and production.
MX366112B (en) Cell culture media and methods of antibody production.
EP3279318A4 (en) Human serum albumin-containing culture medium for growth of neural stem cells
EA201490945A1 (en) SET, CONTAINING REPLACER OF SERUM AND LABOR FACTORS
SG11201806177QA (en) Transplant material for treatment of heart disease
MY180645A (en) Microbial production of fatty amines
MX2018003354A (en) Selection of pluripotent cells for production of fertile xy female mice.
MX2021001981A (en) Methods of preparing a poloxamer for use in cell culture medium.
WO2013177011A3 (en) In vitro tumor in dish kit and method
IN2015DN01407A (en)
MX2014003094A (en) Methods of promoting differentiation.
NZ765734A (en) Ph adjustment to improve thaw recovery of cell banks
SG10201811177SA (en) Cell culture methods and media comprising n-acetylcysteine
RU2013151490A (en) METHOD FOR SYNCHRONIZING A CELL CYCLE OF FIBROBLASTS ISSUED FROM HUMAN LEATHER AT STAGE G0 / G1
WO2018155952A3 (en) Method for isolating human brain tissue-derived neural stem cell at high efficiency
WO2015117033A3 (en) Methods of increasing protein production in mammalian cells
EP3800246A4 (en) Method for producing human induced pluripotent stem cells containing exogenous chromosome
MX2016013363A (en) Method for increasing biomass synthesis capacity of microorganisms and modified microorganisms therefrom.
EP3554487A4 (en) Xenobiotic-free culture system to expand human limbal stem cells
MX2018001076A (en) Cellular culture medium free from serum.

Legal Events

Date Code Title Description
HE9A Changing address for correspondence with an applicant
FA92 Acknowledgement of application withdrawn (lack of supplementary materials submitted)

Effective date: 20151103