AU2013298450B2 - Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid - Google Patents

Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid Download PDF

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AU2013298450B2
AU2013298450B2 AU2013298450A AU2013298450A AU2013298450B2 AU 2013298450 B2 AU2013298450 B2 AU 2013298450B2 AU 2013298450 A AU2013298450 A AU 2013298450A AU 2013298450 A AU2013298450 A AU 2013298450A AU 2013298450 B2 AU2013298450 B2 AU 2013298450B2
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cells
tumor cells
spheroids
cell culture
tumor
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AU2013298450A1 (en
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Katharina Pachmann
Ulrich Pachmann
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/30—Electrochemically active labels

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2013298450A 2012-08-03 2013-08-02 Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid Active AU2013298450B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102012213838.2A DE102012213838A1 (de) 2012-08-03 2012-08-03 Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit
DE102012213838.2 2012-08-03
PCT/EP2013/066325 WO2014020169A1 (de) 2012-08-03 2013-08-02 Verfahren zur kultivierung einer subpopulation zirkulierender epithelialer tumorzellen aus einer körperflüssigkeit

Publications (2)

Publication Number Publication Date
AU2013298450A1 AU2013298450A1 (en) 2015-03-12
AU2013298450B2 true AU2013298450B2 (en) 2019-01-17

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AU2013298450A Active AU2013298450B2 (en) 2012-08-03 2013-08-02 Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid

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US (2) US11015172B2 (enExample)
EP (2) EP3330373A1 (enExample)
CN (1) CN104685051A (enExample)
AU (1) AU2013298450B2 (enExample)
DE (1) DE102012213838A1 (enExample)
DK (1) DK2880152T3 (enExample)
ES (1) ES2673874T3 (enExample)
HR (1) HRP20180905T1 (enExample)
HU (1) HUE037771T2 (enExample)
IN (1) IN2015DN01407A (enExample)
PL (1) PL2880152T3 (enExample)
PT (1) PT2880152T (enExample)
SI (1) SI2880152T1 (enExample)
TR (1) TR201808441T4 (enExample)
WO (1) WO2014020169A1 (enExample)

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SG10201707902PA (en) 2014-03-31 2017-11-29 Fisher & Paykel Healthcare Ltd Headgear for respiratory interfaces
EP3128325B1 (de) 2015-08-07 2019-01-02 Ulrich Pachmann Verfahren zur bestimmung einer konzentration epithelialer zellen in einer blutprobe oder aspiratsprobe
CN109196112B (zh) * 2016-06-16 2022-10-04 株式会社日立高新技术 球状体内部细胞状态的分析方法
CN108277255A (zh) * 2018-01-30 2018-07-13 陈晓凤 一种体外活性上皮肿瘤细胞的检测方法
JP6935883B2 (ja) * 2018-07-13 2021-09-15 五稜化薬株式会社 蛍光画像診断の前処理方法
KR102837377B1 (ko) * 2020-12-31 2025-07-23 아카데미아 시니카 세포 배양 시스템, 이의 제조방법 및 용도
US11713441B2 (en) 2020-12-31 2023-08-01 Academia Sinica Cell culture substrates, methods and uses thereof

Citations (1)

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WO2007079293A2 (en) * 2005-11-23 2007-07-12 Hospital For Sick Children Cancer stem cells and uses thereof

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AU5734998A (en) * 1997-01-10 1998-08-03 Life Technologies, Inc. Embryonic stem cell serum replacement
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EP1664340A1 (en) * 2003-08-08 2006-06-07 Canbas Co., Ltd. Sensitivity test to predict efficacy of anti-cancer therapies
CA2447400A1 (en) * 2003-09-12 2005-03-12 The Hospital For Sick Children Brain tumor stem cells
CN102746336B (zh) * 2005-06-29 2015-08-19 施瑞修德制药公司 氨基磷酸酯烷化剂前体药物
GB2442059A (en) * 2006-09-19 2008-03-26 Ist Superiore Sanita Test for cancer of the gastrointestinal tract
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
EP2138571B1 (en) * 2008-06-26 2017-04-12 SpheroTec GmbH Process for the preparation of multicellular spheroids
US20110081664A1 (en) * 2008-10-17 2011-04-07 University Of Massachusetts Multipurpose microfluidic device for mimicking a microenvironment within a tumor
EP2337561A4 (en) * 2008-10-23 2012-04-04 Davos Life Science Pte Ltd USE OF A TOCOTRIENOL COMPOSITION FOR THE PREVENTION OF CANCER
CN102021138B (zh) * 2009-09-15 2012-12-26 中国人民解放军第三军医大学第二附属医院 肿瘤干细胞的分离方法
DE102009047146B4 (de) * 2009-11-25 2012-07-19 Ulrich Pachmann Verfahren zum Vorhersagen des Ansprechens einer Tumorerkrankung auf eine therapeutische Maßnahme
US9439941B2 (en) * 2009-12-14 2016-09-13 The University Of Hong Kong Nano cancer barrier device (NCBD) to immobilize and inhibit the division of metastic cancer stem cells
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US20130078667A1 (en) * 2011-09-22 2013-03-28 Oscar B. Goodman Methods for detecting and collecting circulating tumor cells
EP2785871A4 (en) * 2011-11-28 2015-08-26 Baylor College Medicine CTC BIOMARKET TEST FOR COMBATING BRAIN METETAS IN BREAST CANCER
JP6509745B2 (ja) * 2013-01-25 2019-05-08 エックスセル・バイオサイエンシズ・インコーポレイテッド 標的細胞の選択的富化のための方法、組成物、キット、及びシステム

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Non-Patent Citations (2)

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WANG N et al., "Detection of circulating tumor cells and tumor stem cells in patients with breast cancer by using flow cytometry", TUMOR BIOLOGY, (2012), vol. 33, no. 2, doi:10.1007/s13277-011-0303-1, ISSN 1010-4283, pages 561-569. *

Also Published As

Publication number Publication date
AU2013298450A1 (en) 2015-03-12
WO2014020169A1 (de) 2014-02-06
DK2880152T3 (en) 2018-06-25
HRP20180905T1 (hr) 2018-08-24
EP2880152B1 (de) 2018-03-14
HUE037771T2 (hu) 2018-09-28
US11015172B2 (en) 2021-05-25
PL2880152T3 (pl) 2018-10-31
TR201808441T4 (tr) 2018-07-23
IN2015DN01407A (enExample) 2015-07-03
CN104685051A (zh) 2015-06-03
EP3330373A1 (de) 2018-06-06
DE102012213838A1 (de) 2014-02-06
EP2880152A1 (de) 2015-06-10
PT2880152T (pt) 2018-06-20
US20150299663A1 (en) 2015-10-22
ES2673874T3 (es) 2018-06-26
SI2880152T1 (sl) 2018-09-28
US20210238554A1 (en) 2021-08-05
US11920163B2 (en) 2024-03-05

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