TR201808441T4 - Bir vücut sıvısından bir sirküle epitelyal tümör hücreleri alt popülasyonunun kültivasyonu için yöntem. - Google Patents
Bir vücut sıvısından bir sirküle epitelyal tümör hücreleri alt popülasyonunun kültivasyonu için yöntem. Download PDFInfo
- Publication number
- TR201808441T4 TR201808441T4 TR2018/08441T TR201808441T TR201808441T4 TR 201808441 T4 TR201808441 T4 TR 201808441T4 TR 2018/08441 T TR2018/08441 T TR 2018/08441T TR 201808441 T TR201808441 T TR 201808441T TR 201808441 T4 TR201808441 T4 TR 201808441T4
- Authority
- TR
- Turkey
- Prior art keywords
- cells
- tumor
- body fluid
- cultivation
- tumor cells
- Prior art date
Links
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 24
- 210000001124 body fluid Anatomy 0.000 title claims abstract description 22
- 239000010839 body fluid Substances 0.000 title claims abstract description 22
- 210000004027 cell Anatomy 0.000 claims abstract description 69
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 46
- 241000282414 Homo sapiens Species 0.000 claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims abstract description 14
- 239000000725 suspension Substances 0.000 claims abstract description 12
- 210000003855 cell nucleus Anatomy 0.000 claims abstract description 7
- 239000006143 cell culture medium Substances 0.000 claims description 17
- 238000004113 cell culture Methods 0.000 claims description 12
- 230000035755 proliferation Effects 0.000 claims description 9
- 210000003743 erythrocyte Anatomy 0.000 claims description 7
- 239000003102 growth factor Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 210000005259 peripheral blood Anatomy 0.000 claims description 5
- 239000011886 peripheral blood Substances 0.000 claims description 5
- 210000000448 cultured tumor cell Anatomy 0.000 claims description 2
- 210000002751 lymph Anatomy 0.000 claims description 2
- 238000001356 surgical procedure Methods 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 description 11
- 230000001225 therapeutic effect Effects 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 description 6
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 description 6
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- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 6
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 210000002919 epithelial cell Anatomy 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
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- 102000004877 Insulin Human genes 0.000 description 3
- 108090001061 Insulin Proteins 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 3
- 235000019270 ammonium chloride Nutrition 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
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- 239000012894 fetal calf serum Substances 0.000 description 3
- 229940125396 insulin Drugs 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
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- 239000012091 fetal bovine serum Substances 0.000 description 2
- 239000003324 growth hormone secretagogue Substances 0.000 description 2
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
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- 239000008188 pellet Substances 0.000 description 2
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 2
- 235000015497 potassium bicarbonate Nutrition 0.000 description 2
- 239000011736 potassium bicarbonate Substances 0.000 description 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 208000009956 adenocarcinoma Diseases 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 210000000069 breast epithelial cell Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000001794 hormone therapy Methods 0.000 description 1
- 229960000890 hydrocortisone Drugs 0.000 description 1
- 229940010129 hydrocortisone 100 mg Drugs 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
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- 230000001900 immune effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
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- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
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- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 229940086066 potassium hydrogencarbonate Drugs 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000012090 serum-supplement Substances 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
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- 239000007787 solid Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 210000005102 tumor initiating cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0695—Stem cells; Progenitor cells; Precursor cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2523/00—Culture process characterised by temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/30—Electrochemically active labels
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Oncology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Developmental Biology & Embryology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102012213838.2A DE102012213838A1 (de) | 2012-08-03 | 2012-08-03 | Verfahren zur Kultivierung einer Subpopulation zirkulierender epithelialer Tumorzellen aus einer Körperflüssigkeit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TR201808441T4 true TR201808441T4 (tr) | 2018-07-23 |
Family
ID=48916061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TR2018/08441T TR201808441T4 (tr) | 2012-08-03 | 2013-08-02 | Bir vücut sıvısından bir sirküle epitelyal tümör hücreleri alt popülasyonunun kültivasyonu için yöntem. |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US11015172B2 (enExample) |
| EP (2) | EP3330373A1 (enExample) |
| CN (1) | CN104685051A (enExample) |
| AU (1) | AU2013298450B2 (enExample) |
| DE (1) | DE102012213838A1 (enExample) |
| DK (1) | DK2880152T3 (enExample) |
| ES (1) | ES2673874T3 (enExample) |
| HR (1) | HRP20180905T1 (enExample) |
| HU (1) | HUE037771T2 (enExample) |
| IN (1) | IN2015DN01407A (enExample) |
| PL (1) | PL2880152T3 (enExample) |
| PT (1) | PT2880152T (enExample) |
| SI (1) | SI2880152T1 (enExample) |
| TR (1) | TR201808441T4 (enExample) |
| WO (1) | WO2014020169A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201707902PA (en) | 2014-03-31 | 2017-11-29 | Fisher & Paykel Healthcare Ltd | Headgear for respiratory interfaces |
| EP3128325B1 (de) | 2015-08-07 | 2019-01-02 | Ulrich Pachmann | Verfahren zur bestimmung einer konzentration epithelialer zellen in einer blutprobe oder aspiratsprobe |
| CN109196112B (zh) * | 2016-06-16 | 2022-10-04 | 株式会社日立高新技术 | 球状体内部细胞状态的分析方法 |
| CN108277255A (zh) * | 2018-01-30 | 2018-07-13 | 陈晓凤 | 一种体外活性上皮肿瘤细胞的检测方法 |
| JP6935883B2 (ja) * | 2018-07-13 | 2021-09-15 | 五稜化薬株式会社 | 蛍光画像診断の前処理方法 |
| KR102837377B1 (ko) * | 2020-12-31 | 2025-07-23 | 아카데미아 시니카 | 세포 배양 시스템, 이의 제조방법 및 용도 |
| US11713441B2 (en) | 2020-12-31 | 2023-08-01 | Academia Sinica | Cell culture substrates, methods and uses thereof |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5734998A (en) * | 1997-01-10 | 1998-08-03 | Life Technologies, Inc. | Embryonic stem cell serum replacement |
| CN1446583A (zh) * | 2002-11-29 | 2003-10-08 | 帕弗瑞生物技术(北京)有限公司 | 一种肿瘤免疫治疗及预防性疫苗的组成、制备、应用方案 |
| EP1664340A1 (en) * | 2003-08-08 | 2006-06-07 | Canbas Co., Ltd. | Sensitivity test to predict efficacy of anti-cancer therapies |
| CA2447400A1 (en) * | 2003-09-12 | 2005-03-12 | The Hospital For Sick Children | Brain tumor stem cells |
| CN102746336B (zh) * | 2005-06-29 | 2015-08-19 | 施瑞修德制药公司 | 氨基磷酸酯烷化剂前体药物 |
| US20070123448A1 (en) * | 2005-11-23 | 2007-05-31 | The Hospital For Sick Children | Novel chemical entities affecting neuroblastoma tumor-initiating cells |
| GB2442059A (en) * | 2006-09-19 | 2008-03-26 | Ist Superiore Sanita | Test for cancer of the gastrointestinal tract |
| FI20075417A0 (fi) * | 2007-06-05 | 2007-06-05 | Marjo-Riitta Suuronen | Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen |
| EP2138571B1 (en) * | 2008-06-26 | 2017-04-12 | SpheroTec GmbH | Process for the preparation of multicellular spheroids |
| US20110081664A1 (en) * | 2008-10-17 | 2011-04-07 | University Of Massachusetts | Multipurpose microfluidic device for mimicking a microenvironment within a tumor |
| EP2337561A4 (en) * | 2008-10-23 | 2012-04-04 | Davos Life Science Pte Ltd | USE OF A TOCOTRIENOL COMPOSITION FOR THE PREVENTION OF CANCER |
| CN102021138B (zh) * | 2009-09-15 | 2012-12-26 | 中国人民解放军第三军医大学第二附属医院 | 肿瘤干细胞的分离方法 |
| DE102009047146B4 (de) * | 2009-11-25 | 2012-07-19 | Ulrich Pachmann | Verfahren zum Vorhersagen des Ansprechens einer Tumorerkrankung auf eine therapeutische Maßnahme |
| US9439941B2 (en) * | 2009-12-14 | 2016-09-13 | The University Of Hong Kong | Nano cancer barrier device (NCBD) to immobilize and inhibit the division of metastic cancer stem cells |
| US20110206735A1 (en) * | 2010-02-24 | 2011-08-25 | Yong Qian | Tumorigenic CancerStemCells, Methods of Isolating and Using the Same |
| US20130078667A1 (en) * | 2011-09-22 | 2013-03-28 | Oscar B. Goodman | Methods for detecting and collecting circulating tumor cells |
| EP2785871A4 (en) * | 2011-11-28 | 2015-08-26 | Baylor College Medicine | CTC BIOMARKET TEST FOR COMBATING BRAIN METETAS IN BREAST CANCER |
| JP6509745B2 (ja) * | 2013-01-25 | 2019-05-08 | エックスセル・バイオサイエンシズ・インコーポレイテッド | 標的細胞の選択的富化のための方法、組成物、キット、及びシステム |
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2012
- 2012-08-03 DE DE102012213838.2A patent/DE102012213838A1/de not_active Withdrawn
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2013
- 2013-08-02 PL PL13745085T patent/PL2880152T3/pl unknown
- 2013-08-02 EP EP18152803.5A patent/EP3330373A1/de active Pending
- 2013-08-02 AU AU2013298450A patent/AU2013298450B2/en active Active
- 2013-08-02 WO PCT/EP2013/066325 patent/WO2014020169A1/de not_active Ceased
- 2013-08-02 TR TR2018/08441T patent/TR201808441T4/tr unknown
- 2013-08-02 HR HRP20180905TT patent/HRP20180905T1/hr unknown
- 2013-08-02 ES ES13745085.4T patent/ES2673874T3/es active Active
- 2013-08-02 CN CN201380050740.XA patent/CN104685051A/zh active Pending
- 2013-08-02 PT PT137450854T patent/PT2880152T/pt unknown
- 2013-08-02 SI SI201331064T patent/SI2880152T1/sl unknown
- 2013-08-02 EP EP13745085.4A patent/EP2880152B1/de active Active
- 2013-08-02 HU HUE13745085A patent/HUE037771T2/hu unknown
- 2013-08-02 DK DK13745085.4T patent/DK2880152T3/en active
- 2013-08-02 US US14/419,149 patent/US11015172B2/en active Active
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2021
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Also Published As
| Publication number | Publication date |
|---|---|
| AU2013298450A1 (en) | 2015-03-12 |
| WO2014020169A1 (de) | 2014-02-06 |
| DK2880152T3 (en) | 2018-06-25 |
| HRP20180905T1 (hr) | 2018-08-24 |
| EP2880152B1 (de) | 2018-03-14 |
| HUE037771T2 (hu) | 2018-09-28 |
| US11015172B2 (en) | 2021-05-25 |
| PL2880152T3 (pl) | 2018-10-31 |
| IN2015DN01407A (enExample) | 2015-07-03 |
| CN104685051A (zh) | 2015-06-03 |
| EP3330373A1 (de) | 2018-06-06 |
| DE102012213838A1 (de) | 2014-02-06 |
| EP2880152A1 (de) | 2015-06-10 |
| PT2880152T (pt) | 2018-06-20 |
| US20150299663A1 (en) | 2015-10-22 |
| ES2673874T3 (es) | 2018-06-26 |
| SI2880152T1 (sl) | 2018-09-28 |
| US20210238554A1 (en) | 2021-08-05 |
| AU2013298450B2 (en) | 2019-01-17 |
| US11920163B2 (en) | 2024-03-05 |
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