DE102010044013A1 - Tiefenauflösungsgesteigerte Mikroskopie - Google Patents
Tiefenauflösungsgesteigerte Mikroskopie Download PDFInfo
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- DE102010044013A1 DE102010044013A1 DE102010044013A DE102010044013A DE102010044013A1 DE 102010044013 A1 DE102010044013 A1 DE 102010044013A1 DE 102010044013 A DE102010044013 A DE 102010044013A DE 102010044013 A DE102010044013 A DE 102010044013A DE 102010044013 A1 DE102010044013 A1 DE 102010044013A1
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6447—Fluorescence; Phosphorescence by visual observation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102010044013A DE102010044013A1 (de) | 2010-11-16 | 2010-11-16 | Tiefenauflösungsgesteigerte Mikroskopie |
| US13/885,979 US9201011B2 (en) | 2010-11-16 | 2011-11-11 | Increased depth-resolution microscopy |
| PCT/EP2011/069918 WO2012065910A1 (de) | 2010-11-16 | 2011-11-11 | Tiefenauflösungsgesteigerte mikroskopie |
| JP2013539209A JP5894180B2 (ja) | 2010-11-16 | 2011-11-11 | 深さ分解能が向上した顕微鏡検査 |
| EP20110782136 EP2641078B1 (de) | 2010-11-16 | 2011-11-11 | Tiefenauflösungsgesteigerte mikroskopie |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102010044013A DE102010044013A1 (de) | 2010-11-16 | 2010-11-16 | Tiefenauflösungsgesteigerte Mikroskopie |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE102010044013A1 true DE102010044013A1 (de) | 2012-05-16 |
Family
ID=44947101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE102010044013A Withdrawn DE102010044013A1 (de) | 2010-11-16 | 2010-11-16 | Tiefenauflösungsgesteigerte Mikroskopie |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9201011B2 (enExample) |
| EP (1) | EP2641078B1 (enExample) |
| JP (1) | JP5894180B2 (enExample) |
| DE (1) | DE102010044013A1 (enExample) |
| WO (1) | WO2012065910A1 (enExample) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012201003A1 (de) * | 2012-01-24 | 2013-07-25 | Carl Zeiss Microscopy Gmbh | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
| EP2803978A1 (de) * | 2013-05-14 | 2014-11-19 | Carl Zeiss Microscopy GmbH | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| EP2803977A1 (de) | 2013-05-14 | 2014-11-19 | Carl Zeiss Microscopy GmbH | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| WO2014191382A1 (de) * | 2013-05-28 | 2014-12-04 | Carl Zeiss Microscopy Gmbh | Lumineszenzmikroskopie |
| DE102013106895A1 (de) * | 2013-07-01 | 2015-01-08 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| WO2015022146A1 (de) * | 2013-08-14 | 2015-02-19 | Carl Zeiss Microscopy Gmbh | Hochauflösende 3d-fluoreszenzmikroskopie |
| EP2943778A4 (en) * | 2013-01-09 | 2016-09-28 | Univ California | DEVICE AND METHOD FOR FLUORESCENT IMAGING WITH RADIO FREQUENCY MULTIPLEX ATTRACTION |
| US10006852B2 (en) | 2016-09-13 | 2018-06-26 | Becton, Dickinson And Company | Flow cytometer with optical equalization |
| US10036699B2 (en) | 2014-03-18 | 2018-07-31 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US10078045B2 (en) | 2015-10-13 | 2018-09-18 | Omega Biosystems Incorporated | Multi-modal fluorescence imaging flow cytometry system |
| US10324019B2 (en) | 2016-03-17 | 2019-06-18 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| DE102018220779A1 (de) * | 2018-12-03 | 2020-06-04 | Carl Zeiss Microscopy Gmbh | Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe |
| US10935485B2 (en) | 2016-05-12 | 2021-03-02 | Bd Biosciences | Fluorescence imaging flow cytometry with enhanced image resolution |
| US11513058B2 (en) | 2020-05-19 | 2022-11-29 | Becton, Dickinson And Company | Methods for modulating an intensity profile of a laser beam and systems for same |
| US11680889B2 (en) | 2020-06-26 | 2023-06-20 | Becton, Dickinson And Company | Dual excitation beams for irradiating a sample in a flow stream and methods for using same |
| US11978269B2 (en) | 2019-07-10 | 2024-05-07 | Becton, Dickinson And Company | Reconfigurable integrated circuits for adjusting cell sorting classification |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012200344A1 (de) | 2012-01-11 | 2013-07-11 | Carl Zeiss Microscopy Gmbh | Mikroskopsystem und Verfahren für die 3-D hochauflösende Mikroskopie |
| JP5900515B2 (ja) | 2012-01-18 | 2016-04-06 | 株式会社ニコン | 構造化照明装置、構造化照明顕微鏡装置、構造化照明方法 |
| DE102013102988A1 (de) * | 2013-03-22 | 2014-09-25 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| JP6223157B2 (ja) * | 2013-12-04 | 2017-11-01 | オリンパス株式会社 | 三次元画像撮像方法、三次元画像解析方法、及び三次元画像撮像システム |
| DE102013226277A1 (de) * | 2013-12-17 | 2015-06-18 | Leica Microsystems Cms Gmbh | Verfahren und Vorrichtung zum Untersuchen einer Probe mittels optischer Projektionstomografie |
| US20150198794A1 (en) | 2014-01-14 | 2015-07-16 | Applied Scientific Instrumentation, Inc. | Light sheet generator |
| EP2963672A1 (en) * | 2014-06-30 | 2016-01-06 | FEI Company | Computational scanning microscopy with improved resolution |
| JP6510239B2 (ja) * | 2015-01-08 | 2019-05-08 | オリンパス株式会社 | 蛍光顕微鏡 |
| DE102015209756A1 (de) * | 2015-05-28 | 2016-12-01 | Carl Zeiss Microscopy Gmbh | Anordnung und Verfahren zur Lichtblattmikroskopie |
| WO2017180680A1 (en) * | 2016-04-12 | 2017-10-19 | The Board Of Regents Of The University Of Texas System | LIGHT-SHEET MICROSCOPE WITH PARALLELIZED 3D lMAGE ACQUISITION |
| FR3055704B1 (fr) * | 2016-09-02 | 2018-10-05 | Centre National De La Recherche Scientifique | Procede de detection d'especes fluorescentes reversiblement photocommutables a haute frequence |
| US10690898B2 (en) * | 2016-09-15 | 2020-06-23 | Molecular Devices (Austria) GmbH | Light-field microscope with selective-plane illumination |
| JP6932036B2 (ja) * | 2017-07-31 | 2021-09-08 | シスメックス株式会社 | 細胞撮像方法、細胞撮像装置、粒子撮像方法および粒子撮像装置 |
| JP2019086529A (ja) * | 2017-11-01 | 2019-06-06 | 株式会社ニコン | 顕微鏡、照明装置、及び観察方法 |
| WO2020121007A2 (en) * | 2018-12-14 | 2020-06-18 | Eötvös Loránd Tudományegyetem | Assembly for switching optical path and optical microscope including the assembly |
| DE102020202821A1 (de) * | 2020-03-05 | 2021-09-09 | Karlsruher Institut für Technologie | Lokalisierung von optischen Koppelstellen |
| JP7568210B2 (ja) * | 2020-05-22 | 2024-10-16 | 国立大学法人山梨大学 | 分析システム及び分析方法 |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006127692A2 (en) | 2005-05-23 | 2006-11-30 | Hess Harald F | Optical microscopy with phototransformable optical labels |
| WO2007009812A1 (de) | 2005-07-22 | 2007-01-25 | Carl Zeiss Microimaging Gmbh | Auflösungsgesteigerte lumineszenz-mikroskopie |
| US20090134342A1 (en) * | 2006-05-06 | 2009-05-28 | Stefan Hell | High spatial resolution imaging of a structure of interest in a specimen |
| DE102008008216A1 (de) | 2008-02-08 | 2009-08-20 | Monika Meindl | Hygieneeinsatz für Steckbecken auf genannt Bettpfanne, Schieber, Steckbecken |
| DE102008024568A1 (de) * | 2008-05-21 | 2009-12-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum räumlich hochauflösenden Abbilden einer interessierenden Struktur einer Probe |
| US20100062460A1 (en) * | 2008-09-08 | 2010-03-11 | Praveen Pande | Novel fluorochromes for organelle tracing and multi-color imaging |
| US20100160613A1 (en) * | 2008-12-22 | 2010-06-24 | Leica Microsystems Cms Gmbh | Method for high spatial resolution stochastic examination of a sample structure labeled with a substance |
| US20100207037A1 (en) * | 2009-01-26 | 2010-08-19 | The General Hospital Corporation | System, method and computer-accessible medium for providing wide-field superresolution microscopy |
| US20100265318A1 (en) * | 2008-04-01 | 2010-10-21 | The Jackson Laboratory | 3D Biplane Microscopy |
| US20100283835A1 (en) * | 2007-01-11 | 2010-11-11 | Joerg Bewersdorf | Microscopic imaging techniques |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10257423A1 (de) * | 2002-12-09 | 2004-06-24 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Mikroskop |
| DE102004034987A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop und Verwendung |
| JP5012071B2 (ja) * | 2007-02-14 | 2012-08-29 | 株式会社ニコン | スリット走査共焦点顕微鏡 |
| WO2009085218A1 (en) * | 2007-12-21 | 2009-07-09 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution in three dimensions |
| DE102008009216A1 (de) | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| DE102008018476B4 (de) * | 2008-04-11 | 2022-12-15 | Carl Zeiss Microscopy Gmbh | Mikroskopievorrichtung |
| DE102008021641A1 (de) * | 2008-04-30 | 2009-11-05 | Carl Zeiss Microlmaging Gmbh | Auflösungsgesteigerte Lumineszenzmikroskopie |
| JP5208825B2 (ja) * | 2008-09-12 | 2013-06-12 | オリンパス株式会社 | 光学顕微鏡 |
| US7675045B1 (en) * | 2008-10-09 | 2010-03-09 | Los Alamos National Security, Llc | 3-dimensional imaging at nanometer resolutions |
-
2010
- 2010-11-16 DE DE102010044013A patent/DE102010044013A1/de not_active Withdrawn
-
2011
- 2011-11-11 US US13/885,979 patent/US9201011B2/en active Active
- 2011-11-11 WO PCT/EP2011/069918 patent/WO2012065910A1/de not_active Ceased
- 2011-11-11 JP JP2013539209A patent/JP5894180B2/ja active Active
- 2011-11-11 EP EP20110782136 patent/EP2641078B1/de active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006127692A2 (en) | 2005-05-23 | 2006-11-30 | Hess Harald F | Optical microscopy with phototransformable optical labels |
| WO2007009812A1 (de) | 2005-07-22 | 2007-01-25 | Carl Zeiss Microimaging Gmbh | Auflösungsgesteigerte lumineszenz-mikroskopie |
| US20090134342A1 (en) * | 2006-05-06 | 2009-05-28 | Stefan Hell | High spatial resolution imaging of a structure of interest in a specimen |
| US20100283835A1 (en) * | 2007-01-11 | 2010-11-11 | Joerg Bewersdorf | Microscopic imaging techniques |
| DE102008008216A1 (de) | 2008-02-08 | 2009-08-20 | Monika Meindl | Hygieneeinsatz für Steckbecken auf genannt Bettpfanne, Schieber, Steckbecken |
| US20100265318A1 (en) * | 2008-04-01 | 2010-10-21 | The Jackson Laboratory | 3D Biplane Microscopy |
| DE102008024568A1 (de) * | 2008-05-21 | 2009-12-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum räumlich hochauflösenden Abbilden einer interessierenden Struktur einer Probe |
| US20100062460A1 (en) * | 2008-09-08 | 2010-03-11 | Praveen Pande | Novel fluorochromes for organelle tracing and multi-color imaging |
| US20100160613A1 (en) * | 2008-12-22 | 2010-06-24 | Leica Microsystems Cms Gmbh | Method for high spatial resolution stochastic examination of a sample structure labeled with a substance |
| US20100207037A1 (en) * | 2009-01-26 | 2010-08-19 | The General Hospital Corporation | System, method and computer-accessible medium for providing wide-field superresolution microscopy |
Non-Patent Citations (9)
| Title |
|---|
| Fahrbach, F. O., Rohrbach, A., "A line scanned light-sheet microscope with phase shaped self-reconstructing beams", Optics Express 24229, Vol. 18, Nr. 23, 2010 |
| Gurskaya et al., Nature Biotech., Band 24, S. 461-465, 2006 |
| Huan et al., Science 319, Seite 810, 2008 |
| HUANG,B.et.al.:Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy.In:SCIENCE,Feb.2008, Vol.319,S.810-813 ges.Dok. * |
| Juette et al., Nature Methods 5, Seite 527, 2008 |
| P. Keller und E. Stelzer, "Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence Microscopy", Current Opinion in Neurobiology, 2009, Vol. 19, S. 1-9 |
| Pavani et al., PNAS 106, Seite 2995, 2009 |
| Shtengel et al., PNAS 106, Seite 3125, 2009 |
| Toprak et al., Nanolet. 7, Seiten 3285-3290, 2007 |
Cited By (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012201003B4 (de) | 2012-01-24 | 2024-07-25 | Carl Zeiss Microscopy Gmbh | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
| US9885860B2 (en) | 2012-01-24 | 2018-02-06 | Carl Zeiss Microscopy Gmbh | Microscope and method for high-resolution 3D fluorescence microscopy |
| DE102012201003A1 (de) * | 2012-01-24 | 2013-07-25 | Carl Zeiss Microscopy Gmbh | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
| US10408758B2 (en) | 2013-01-09 | 2019-09-10 | The Regents Of The University Of California | Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation |
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| US11327016B2 (en) | 2013-01-09 | 2022-05-10 | The Regents Of The University Of California | Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation |
| US11371937B2 (en) | 2013-01-09 | 2022-06-28 | The Regents Of The University Of California | Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation |
| EP2943778A4 (en) * | 2013-01-09 | 2016-09-28 | Univ California | DEVICE AND METHOD FOR FLUORESCENT IMAGING WITH RADIO FREQUENCY MULTIPLEX ATTRACTION |
| US9983132B2 (en) | 2013-01-09 | 2018-05-29 | The Regents Of The University Of California | Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation |
| US12158422B2 (en) | 2013-01-09 | 2024-12-03 | The Regents Of The University Of California | Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation |
| JP2014224813A (ja) * | 2013-05-14 | 2014-12-04 | カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ | ３ｄ高解像度局在顕微鏡法のための方法 |
| JP2014224814A (ja) * | 2013-05-14 | 2014-12-04 | カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ | ３ｄ高解像度局在顕微鏡法のための方法 |
| DE102013208926A1 (de) | 2013-05-14 | 2014-11-20 | Carl Zeiss Microscopy Gmbh | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| EP2803977A1 (de) | 2013-05-14 | 2014-11-19 | Carl Zeiss Microscopy GmbH | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| US10024793B2 (en) | 2013-05-14 | 2018-07-17 | Carl Zeiss Microscopy Gmbh | Method for three-dimensional high resolution localization microscopy |
| EP2803978A1 (de) * | 2013-05-14 | 2014-11-19 | Carl Zeiss Microscopy GmbH | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| US9857581B2 (en) | 2013-05-28 | 2018-01-02 | Carl Zeiss Microscopy Gmbh | Luminescence microscopy |
| EP3373063A1 (de) * | 2013-05-28 | 2018-09-12 | Carl Zeiss Microscopy GmbH | Lumineszenzmikroskopie |
| WO2014191382A1 (de) * | 2013-05-28 | 2014-12-04 | Carl Zeiss Microscopy Gmbh | Lumineszenzmikroskopie |
| DE102013106895B4 (de) * | 2013-07-01 | 2015-09-17 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| US10234672B2 (en) | 2013-07-01 | 2019-03-19 | Leica Microsystems Cms Gmbh | Light-microscopic method of localization microscopy for localizing point objects |
| DE102013106895A1 (de) * | 2013-07-01 | 2015-01-08 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| CN105452931A (zh) * | 2013-08-14 | 2016-03-30 | 卡尔蔡司显微镜有限责任公司 | 高分辨率3d荧光显微术 |
| WO2015022146A1 (de) * | 2013-08-14 | 2015-02-19 | Carl Zeiss Microscopy Gmbh | Hochauflösende 3d-fluoreszenzmikroskopie |
| US10222316B2 (en) | 2014-03-18 | 2019-03-05 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US11946851B2 (en) | 2014-03-18 | 2024-04-02 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US12055476B2 (en) | 2014-03-18 | 2024-08-06 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US11630053B2 (en) | 2014-03-18 | 2023-04-18 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US10451538B2 (en) | 2014-03-18 | 2019-10-22 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US10036699B2 (en) | 2014-03-18 | 2018-07-31 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US10845295B2 (en) | 2014-03-18 | 2020-11-24 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US11280718B2 (en) | 2014-03-18 | 2022-03-22 | The Regents Of The University Of California | Parallel flow cytometer using radiofrequency multiplexing |
| US10684211B2 (en) | 2015-10-13 | 2020-06-16 | Omega Biosystems Incorporated | Multi-modal fluorescence imaging flow cytometry system |
| US11940369B2 (en) | 2015-10-13 | 2024-03-26 | Becton, Dickinson And Company | Multi-modal fluorescence imaging flow cytometry system |
| US10935482B2 (en) | 2015-10-13 | 2021-03-02 | Becton, Dickinson And Company | Multi-modal fluorescence imaging flow cytometry system |
| US11692926B2 (en) | 2015-10-13 | 2023-07-04 | Becton, Dickinson And Company | Multi-modal fluorescence imaging flow cytometry system |
| US11366052B2 (en) | 2015-10-13 | 2022-06-21 | Becton, Dickinson And Company | Multi-modal fluorescence imaging flow cytometry system |
| US10078045B2 (en) | 2015-10-13 | 2018-09-18 | Omega Biosystems Incorporated | Multi-modal fluorescence imaging flow cytometry system |
| US12038372B2 (en) | 2015-10-13 | 2024-07-16 | Becton, Dickinson And Company | Multi-modal fluorescence imaging flow cytometry system |
| US10288546B2 (en) | 2015-10-13 | 2019-05-14 | Omega Biosystems Incorporated | Multi-modal fluorescence imaging flow cytometry system |
| US11774343B2 (en) | 2016-03-17 | 2023-10-03 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| US10620111B2 (en) | 2016-03-17 | 2020-04-14 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| US12313521B1 (en) | 2016-03-17 | 2025-05-27 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| US10324019B2 (en) | 2016-03-17 | 2019-06-18 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| US11105728B2 (en) | 2016-03-17 | 2021-08-31 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| US12078587B2 (en) | 2016-03-17 | 2024-09-03 | Becton, Dickinson And Company | Cell sorting using a high throughput fluorescence flow cytometer |
| US10935485B2 (en) | 2016-05-12 | 2021-03-02 | Bd Biosciences | Fluorescence imaging flow cytometry with enhanced image resolution |
| US10823658B2 (en) | 2016-09-13 | 2020-11-03 | Becton, Dickinson And Company | Flow cytometer with optical equalization |
| US10006852B2 (en) | 2016-09-13 | 2018-06-26 | Becton, Dickinson And Company | Flow cytometer with optical equalization |
| US11698334B2 (en) | 2016-09-13 | 2023-07-11 | Becton, Dickinson And Company | Flow cytometer with optical equalization |
| DE102018220779A1 (de) * | 2018-12-03 | 2020-06-04 | Carl Zeiss Microscopy Gmbh | Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe |
| US11978269B2 (en) | 2019-07-10 | 2024-05-07 | Becton, Dickinson And Company | Reconfigurable integrated circuits for adjusting cell sorting classification |
| US11513058B2 (en) | 2020-05-19 | 2022-11-29 | Becton, Dickinson And Company | Methods for modulating an intensity profile of a laser beam and systems for same |
| US12306089B2 (en) | 2020-05-19 | 2025-05-20 | Becton, Dickinson And Company | Methods for modulating an intensity profile of a laser beam and systems for same |
| US11971343B2 (en) | 2020-06-26 | 2024-04-30 | Becton, Dickinson And Company | Dual excitation beams for irradiating a sample in a flow stream and methods for using same |
| US11680889B2 (en) | 2020-06-26 | 2023-06-20 | Becton, Dickinson And Company | Dual excitation beams for irradiating a sample in a flow stream and methods for using same |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012065910A1 (de) | 2012-05-24 |
| JP5894180B2 (ja) | 2016-03-23 |
| US9201011B2 (en) | 2015-12-01 |
| EP2641078A1 (de) | 2013-09-25 |
| US20130302905A1 (en) | 2013-11-14 |
| JP2014501915A (ja) | 2014-01-23 |
| EP2641078B1 (de) | 2015-01-07 |
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